Team:IPN-UNAM-Mexico/Notebook

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As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into ''E. coli''. For this reason we had to delay lab work and request the 2009 catalog.
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<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Protocols|Protocols]]</h1>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/July|July]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/August|August]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/September|September]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/October|October]]</h2>
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<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Safety|Safety]]</h1>
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Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is  described below:
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{| border="2"
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!Number
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!Biobrick
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!Restriction
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|-
 +
|2
 +
|BBa_R0079
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|S & P
 +
|-
 +
|3
 +
|BBa_F1610
 +
|X & P
 +
|-
 +
|4
 +
|BBa_K091146
 +
|S & P
 +
|-
 +
|5
 +
|BBa_K093005
 +
|X &
 +
|-
 +
|6
 +
|BBa_K081016
 +
|X & P
 +
|-
 +
|7
 +
|BBa_EC840
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|X & P
 +
|-
 +
|8
 +
|BBa_K081009
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|X & P
 +
|-
 +
|9
 +
|BBa_R0051
 +
|S &P
 +
|-
 +
|10
 +
|BBa_J06800
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|X & P
 +
|-
 +
|11
 +
|BBa_Q04121
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|X & P
 +
|-
 +
|12
 +
|BBa_B0034
 +
|S & P
 +
|-
 +
|13
 +
|BBa_C0079
 +
|E & S
 +
|-
 +
|14
 +
|BBa_C0179
 +
|E & S
 +
|-
 +
|15
 +
|BBa_B0015
 +
|E & X
 +
|-
 +
|16
 +
|BBa_K081018
 +
|E & S
 +
|-
 +
|17
 +
|BBa_K116640
 +
|X & P
 +
|-
 +
|23
 +
|BBa_S03154
 +
|X & P
 +
|-
 +
|24
 +
|BBa_k145201
 +
|E & S
 +
|}
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</center>
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{| border="2"
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! Ligation
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! Nickname
 +
! Biobrick
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|-
 +
|4-23
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|Ciencias I
 +
|BBa_K226005
 +
|-
 +
|13-15
 +
|Ciencias II
 +
|BBa_K266002
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|-
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|Ciencias I-7
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|Ciencias III
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|BBa_K266006
 +
|-
 +
|24-17
 +
|Ciencias IV
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|BBa_K266001
 +
|-
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|2-3
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|Ciencias V
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|BBa_K266000
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|-
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|J231000-11
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|Ciencias VI
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|BBa_K266008
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|-
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|Amplicon - Ciencias II
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|Ciencias VII
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|BBa_K266003
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|-
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|Ciencias VI - 12
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|Ciencias VIII
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|BBa_K266009
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|-
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|Ciencias III - 19
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|Ciencias IX
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|BBa_K266006
 +
|-
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|J23100 – Ciencias VII
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|Ciencias X
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|BBa_K266004
 +
|-
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|Ciencias III - Ciencias V
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|Ciencias XI
 +
|BBa_K266007
 +
|-
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|Ciencias X - Ciencias IV
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|Ciencias XII
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|BBa_K266010
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|}</center>
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Latest revision as of 01:29, 22 October 2009


BannerUNAM.jpg

NOTEBOOK

As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into E. coli. For this reason we had to delay lab work and request the 2009 catalog.

Month-icon.pngProtocols

Month-icon.pngJuly

Month-icon.pngAugust

Month-icon.pngSeptember

Month-icon.pngOctober

Month-icon.pngSafety

Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:


Number Biobrick Restriction
2 BBa_R0079 S & P
3 BBa_F1610 X & P
4 BBa_K091146 S & P
5 BBa_K093005 X &
6 BBa_K081016 X & P
7 BBa_EC840 X & P
8 BBa_K081009 X & P
9 BBa_R0051 S &P
10 BBa_J06800 X & P
11 BBa_Q04121 X & P
12 BBa_B0034 S & P
13 BBa_C0079 E & S
14 BBa_C0179 E & S
15 BBa_B0015 E & X
16 BBa_K081018 E & S
17 BBa_K116640 X & P
23 BBa_S03154 X & P
24 BBa_k145201 E & S


Ligation Nickname Biobrick
4-23 Ciencias I BBa_K226005
13-15 Ciencias II BBa_K266002
Ciencias I-7 Ciencias III BBa_K266006
24-17 Ciencias IV BBa_K266001
2-3 Ciencias V BBa_K266000
J231000-11 Ciencias VI BBa_K266008
Amplicon - Ciencias II Ciencias VII BBa_K266003
Ciencias VI - 12 Ciencias VIII BBa_K266009
Ciencias III - 19 Ciencias IX BBa_K266006
J23100 – Ciencias VII Ciencias X BBa_K266004
Ciencias III - Ciencias V Ciencias XI BBa_K266007
Ciencias X - Ciencias IV Ciencias XII BBa_K266010

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