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Duke University/4 June 2009
From 2009.igem.org
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| - | + | [[Team:Duke | Return ]] | |
| - | + | ||
| + | ==June 4, 2009== | ||
| + | |||
| + | <pre> | ||
| + | E: Gel to check Bioplastics #1-5 | ||
| + | R: All correct sized bands | ||
| + | C: Use one as template for PCR | ||
| + | |||
| + | E: PCR with Bioplastics #5 as template | ||
| + | |||
| + | 5x HF buffer 5 ul | ||
| + | dNTPs 2 ul | ||
| + | forward primer 1.25 ul | ||
| + | reverse primer 1.25 ul | ||
| + | DNA template (plasmid) 0.5 ul | ||
| + | Phusion DNA polymerase 0.3 ul | ||
| + | H2O 14.7 ul | ||
| + | 25 ul | ||
| + | |||
| + | 98°C 30s 1x | ||
| + | 98°C 15s | ||
| + | 55°C 30s | ||
| + | 72°C 1m 10s 35x | ||
| + | 72°C 5m | ||
| + | 4°C infinity 1x | ||
| + | |||
| + | Fragment # Name Size/Length | ||
| + | 1 pASKphaA 4.2 kb | ||
| + | 2 phaB 900 bp | ||
| + | 3 Termcat2 1.7 kb | ||
| + | 4 phaC 1.9 kb | ||
| + | |||
| + | </pre> | ||
Latest revision as of 16:29, 21 October 2009
June 4, 2009
E: Gel to check Bioplastics #1-5 R: All correct sized bands C: Use one as template for PCR E: PCR with Bioplastics #5 as template 5x HF buffer 5 ul dNTPs 2 ul forward primer 1.25 ul reverse primer 1.25 ul DNA template (plasmid) 0.5 ul Phusion DNA polymerase 0.3 ul H2O 14.7 ul 25 ul 98°C 30s 1x 98°C 15s 55°C 30s 72°C 1m 10s 35x 72°C 5m 4°C infinity 1x Fragment # Name Size/Length 1 pASKphaA 4.2 kb 2 phaB 900 bp 3 Termcat2 1.7 kb 4 phaC 1.9 kb
