Team:Nevada/Parts

From 2009.igem.org

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<div style="padding: 10px;">
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<div style="text-align: center;"><big><big><b
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style="color: rgb(0, 0, 0); text-decoration: underline;">Parts</b></big></big><b
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style="color: rgb(0, 0, 0);">&nbsp;&nbsp;&nbsp; <br>
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Registry Parts Used&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;New Parts Added to Registry<br>
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</b>
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<table
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style="text-align: left; width: 100%; background-color: rgb(0, 0, 100);"
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border="2" cellpadding="3" cellspacing="3">
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<tbody>
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<tr>
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<td style="vertical-align: top;"><b><font color="white">Promoters:</b></font><br>
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</td>
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<td style="vertical-align: top;"><b><font color="white">Ribosome Binding Site:</font></b><br>
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</td>
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<td style="vertical-align: top;"><b><font color="white">Terminators:</font></b><br>
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</td>
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<td style="vertical-align: top;">&nbsp;&nbsp;&nbsp;<b><font color="white">Intermediates:</font></b><br>
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</td>
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<tr>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#LacI">LacI</a></li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#RBS">RBS (Elowitz 1999)</a></li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#TERM">Bidirectional Double Terminator</a></li>
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</ul>
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</td>
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<td style="vertical-align: top;">
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<ul>
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<li><a href="#PROMRBS">LacI + RBS<br>
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</a></li>
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<li><a href="#CCRDT">Cinnamoyl-CoA reductase 2 Gene + Double Terminator<br>
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</a></li>
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</ul>
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</td>
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</tr>
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</tbody>
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</table>
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<BR><BR><BR>
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<p class="MsoNormal"
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style="line-height: normal; font-weight: bold; text-align: center; text-decoration: underline;"><big><big>New
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parts:</big></big></p>
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<BR><BR>
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<p class="MsoNormal" style="line-height: normal;"><a name="LacI"></a><b><span
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style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">LacI:
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</span></b><span
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style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">
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We used this part from the part registry.<br>
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<table style="text-align: left; width: 100%; background-color: #0E2451;border:18px solid #0E2451;" cellpadding="3" cellspacing="3">
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For more information go to: <a
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href="http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011"><span
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    <td colspan="3">
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style="color: blue;">BBa_R0011</span></a> </i><o:p></o:p><br>
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      <b style="color: #fff;text-transform:uppercase;font-size:1.2em;letter-spacing:0.1em;line-height: 2em;">Registry Parts Used</b>
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</span></p>
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    </td>
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    <td>
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      <b style="color: #fff;text-transform:uppercase;font-size:1.2em;letter-spacing:0.1em;line-height: 2em;">New Parts Added to Registry</b>
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    </td>
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  </tr>
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  <tr>
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    <td style="vertical-align: top;color:#BEB68E;">
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      <strong>Promoters:</strong>
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    </td>
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    <td style="vertical-align: top;color:#BEB68E;">
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      <strong>Ribosome Binding Site:</strong>
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    </td>
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    <td style="vertical-align: top;color:#BEB68E;">
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      <strong>Terminators:</strong>
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    </td>
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    <td style="vertical-align: top;color:#BEB68E;">
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      <strong>Intermediates:</strong>
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    </td>
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  </tr>
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  <tr>
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    <td style="vertical-align: top;">
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      <ul>
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        <li>
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          <a href="#LacI">LacI</a>
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        </li>
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      </ul>
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    </td>
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    <td style="vertical-align: top;">
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      <ul>
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        <li>
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          <a href="#RBS">RBS (Elowitz 1999)</a>
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        </li>
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      </ul>
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    </td>
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    <td style="vertical-align: top;">
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      <ul>
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        <li>
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          <a href="#TERM">Bidirectional Double Terminator</a>
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        </li>
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      </ul>
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    </td>
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    <td style="vertical-align: top;">
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      <ul>
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        <li>
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          <a href="#PROMRBS">LacI + RBS</a>
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        </li>
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        <li>
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          <a href="#CCRDT">Cinnamoyl-CoA reductase 2 Gene + Double Terminator</a>
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        </li>
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      </ul>
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    </td>
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  </tr>
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</table><br />
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<h2>Registry Parts Used</h2><br />
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<p>
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  <a name="LacI"></a>
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  <b style="font-size: 1.5em; font-family: Times New Roman, sans-serif;">LacI:</b>
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  <span style="font-size: 1.2em; font-family: Times New Roman, sans-serif;">We used this strong IPTG-inducible promoter from the part registry.</span>
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  <i>For more information go to: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011">BBa_R0011</a></i>
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<p><a name="RBS"></a>
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  <b style="font-size: 1.5em; font-family: Times New Roman, sans-serif;">RBS:</b>
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  <span style="font-size: 1.2em; font-family: Times New Roman, sans-serif;">We used this ribosome binding site (based on Elowitz repressilator) from the part registry. It has a relative efficiency of 1.</span>
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  <i>For more information go to: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</a></i>
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</p><br />
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<div style="padding: 10px;">
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<p><a name="TERM"></a>
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  <b style="font-size: 1.5em; font-family: Times New Roman, sans-serif;">Bidirectional Double Terminator:</b>
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  <span style="font-size: 1.2em; font-family: Times New Roman, sans-serif;">We used this Bidirectional Double Terminator from the part registry. Allows for the cessation of translation and the development of a protein expression system.</span>
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  <i>For more information go to: <a href="http://partsregistry.org/Part:BBa_B0014">BBa_B0014</a></i>
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</p><br />
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===Note===
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<h2>New Parts Added to Registry</h2><br />
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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<p><a name="PROMRBS"></a>
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  <b style="font-size: 1.5em; font-family: Times New Roman, sans-serif;">LacI/RBS:</b>
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  <span style="font-size: 1.2em; font-family: Times New Roman, sans-serif;">We created an IPTG-Inducible promoter and efficient RBS intermediate from existing basic parts within the registry. Allows for the development of an inducible protein expression system.</span>
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  <i>For more information go to: <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K262000">BBa_K262000</a></i>
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</p><br />
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<p><a name="CCRDT"></a>
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  <b style="font-size: 1.5em; font-family: Times New Roman, sans-serif;">Cinnamoyl-CoA reductase 2 Gene + Double Terminator:</b>
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  <span style="font-size: 1.2em; font-family: Times New Roman, sans-serif;">We created a new intermediate part for the registry. This intermediate will allow for the development of a Cinnamoyl-CoA reductase 2 enzyme expression system.</span>
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  <i>For more information go to: <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K262002">BBa_K262002</a></i>
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</p>
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Latest revision as of 06:43, 20 October 2009


Registry Parts Used New Parts Added to Registry
Promoters: Ribosome Binding Site: Terminators: Intermediates:

Registry Parts Used


LacI: We used this strong IPTG-inducible promoter from the part registry. For more information go to: BBa_R0011


RBS: We used this ribosome binding site (based on Elowitz repressilator) from the part registry. It has a relative efficiency of 1. For more information go to: BBa_B0034


Bidirectional Double Terminator: We used this Bidirectional Double Terminator from the part registry. Allows for the cessation of translation and the development of a protein expression system. For more information go to: BBa_B0014


New Parts Added to Registry


LacI/RBS: We created an IPTG-Inducible promoter and efficient RBS intermediate from existing basic parts within the registry. Allows for the development of an inducible protein expression system. For more information go to: BBa_K262000


Cinnamoyl-CoA reductase 2 Gene + Double Terminator: We created a new intermediate part for the registry. This intermediate will allow for the development of a Cinnamoyl-CoA reductase 2 enzyme expression system. For more information go to: BBa_K262002


Retrieved from "http://2009.igem.org/Team:Nevada/Parts"