Team:HKU-HKBU/Assembly

From 2009.igem.org

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(Assembly of BactoMotor)
 
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{{Team:HKU-HKBU/header}}
{{Team:HKU-HKBU/header}}
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=Assembly of BactoMotor=
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='''Assembly of BactoMotor'''=
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===Introduction===
 
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Our BactoMotor is comprised of three parts – the Micro-Motor; the Directional Controller and the Speed Controller.
 
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===Prodecures===
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==Introduction==
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Our BactoMotor comprises of three devices –[[Team:HKU-HKBU/Motor_Overview|Micro-Motor]], [[Team:HKU-HKBU/Polar_Expression_Design|Direction Controller]] and [[Team:HKU-HKBU/Speed_Control_Design |Speed Controller]].
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As their names indicate, the [[Team:HKU-HKBU/Motor_Overview|Micro-Motor]] is the core part of the BactoMotor, and it is small (micrometer scale). The [[Team:HKU-HKBU/Polar_Expression_Design|Direction Controller]] ensures that the propelling force generated by the bacteria translates into a coordinated and directional rotation of the motor. The [[Team:HKU-HKBU/Speed_Control_Design |Speed Controller]] is used to control the speed of the Bacto-motor
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# The Micro-Motor will be installed into a microchip container (Fig.1a, 1b) with separated chambers. Only one side of the arms of the motor is coated with Biotin (for the Directional Controller Module).[[Image:HKU-HKBU ass f1a s.PNG|frame|center|600px|'''Fig.1a:''' Photos of the microchips for micro-motor installation; A: A photo showing the chambers and the valve systems; B: Valve 3 closed; C: Valves 4, 5, 6 and 7 are closed while water is injected into the channel; L: Valve 1 and 7 closed, enclosing the crystal violet solution in the lower 4 chambers; P: The photo of the chamber system]][[Image:HKU-HKBU ass f1b.PNG|frame|center|'''Fig.1b:''' A diagram showing the Micro-Motor Module installed into a chamber inside the microchip.]]
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Each device is designed, engineered and tested separately, and functional on its own. After testing and fine-tuning, the devices are '''assembled''' to form the final System BactoMotor. The end-results is a micrometer-scale BactoMotor with unidirectional rotation, its speed controllable.
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# The Bacteria with both Directional Controller and Speed Controller Modules implemented will be introduced to the Micro-Motor (Fig.2). [[Image:HKU-HKBU ass f2.PNG|frame|center|'''Fig.2:''' Introduction of Bacteria into the Micro-Motor]]
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# Under the effect of the Directional Controller, the Bacteria will bind onto the Micro-Motor directionally. (Fig.3a, 3b)[[Image:HKU-HKBU ass f3a.PNG|frame|center|'''Fig.3a:''' The bacteria bounded to the Micro-Motor through action of the Directional Controller Module (Plan View)]][[Image:HKU-HKBU ass f3b.PNG|frame|center|'''Fig.3b:''' A zoomed view of the Directional Controller Module at work.]]
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# As the Bacteria swims, they will push the motor into rotation. (clockwise as demonstrated in Fig. 4a, 4b)[[Image:HKU-HKBU ass f4a.PNG|frame|center|'''Fig.4a:''' The Directional Rotation of the Micro-Motor achieved by the Directional Controller Module]][[Image:HKU-HKBU ass f4b.gif|frame|center|'''Fig.4b:''' An animation showing the BactoMotor in action.]]
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# To utilize the Speed Controller Module, an inducer is added to the Microchip containing the Micro-Motor. Increase in concentration of the inducer will lead to an overall increase in rotational speed of the Bacto-Motor. (Fig. 5a, 5b)[[Image:HKU-HKBU ass f5a.gif|frame|center|'''Fig.5a:''' On additional of 0.5 effective dose of inducer]][[Image:HKU-HKBU ass f5b.gif|frame|center|'''Fig.5b:''' On additional of 1.0 effective dose of inducer]]
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==Prodecures==
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==='''Step 1. Installation of Micro-Motor'''===
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The Micro-Motor will be installed into a microchip container (Fig.1a, 1b) with separated chambers. Only one side of the arms of the motor is coated with Biotin ([[Team:HKU-HKBU/Polar_Expression_Design|see the Directional Controller]]).[[Image:HKU-HKBU_ass_f1a_s2.png|frame|center|400px|'''Figure 1a:''' Photos of the microchips for micro-motor installation; '''A:''' A photo showing the chambers and the valve systems; '''B:''' Valve 3 closed; '''C:''' Valves 4, 5, 6 and 7 are closed while water is injected into the channel; '''D:''' Valve 1 and 7 closed, enclosing the crystal violet solution in the lower 4 chambers; '''E:''' The photo of the chamber system]][[Image:HKU-HKBU ass f1b.PNG|thumb|200px|center|'''Figure 1b:''' A diagram showing the Micro-Motor Module installed into a chamber inside the microchip.]]
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==='''Step 2. Introduction of Bacteria'''===
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The Bacteria with both Directional Controller and Speed Controller Modules implemented will be introduced to the Micro-Motor (Figure 2). [[Image:HKU-HKBU ass f2.PNG|thumb|200px|center|'''Figure 2:''' Introduction of Bacteria into the Micro-Motor]]
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==='''Step 3. Action of Directional Controller'''===
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Under the effect of the Direction Controller, all bacteria will only bind onto the sides coated with biotin on each blade of the Micro-Motor.(Fig.3a, 3b)[[Image:HKU-HKBU ass f3a.PNG|thumb|200px|center|'''Fig.3a:''' The bacteria bounded to the Micro-Motor through action of the Direction Controller Module (Plan View)]][[Image:HKU-HKBU ass f3b.PNG|thumb|200px|center|'''Fig.3b:''' A microscopic view of the Direction Controller Module at work.]]
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==='''Step 4. Removing Excessive Bacteria'''===
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The extra bacterial cells will be removed by medium flush. (Fig. 4)[[Image:HKU-HKBU ass f3s.PNG|thumb|200px|center|'''Fig.4:''' Bacteria not binding to the Micro-Motor will be flushed away by medium flush.]]
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==='''Step 5. BactoMotor in Action'''===
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As the Bacteria swims, they will push the motor into rotation. (clockwise as demonstrated in Fig. 5a, 5b)[[Image:HKU-HKBU ass f4a.PNG|thumb|300px|center|'''Fig.5a:''' The Directional Rotation of the Micro-Motor achieved by the Directional Controller Module]][[Image:HKU-HKBU ass f4b.gif|thumb|200px|center|'''Fig.5b:''' An animation showing the BactoMotor in action.]]
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==='''Step 6. Using the Speed Controller'''===
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To utilize the Speed Controller Module, inducer aTc is added to the Microchip containing the Micro-Motor. Increase in concentration of the inducer aTc will lead to an overall increase in rotational speed of the Bacto-Motor. (Fig. 6a, 6b)[[Image:HKU-HKBU ass f5a.gif|thumb|200px|center|'''Fig.6a:''' On additional of 0.5 effective dose of inducer aTc]][[Image:HKU-HKBU ass f5b.gif|thumb|200px|center|'''Fig.6b:''' On additional of 1.0 effective dose of inducer]]
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On the other hand, dilution/chelation of the inducer will decrease the speed of the motor.
{{Team:HKU-HKBU/footer}}
{{Team:HKU-HKBU/footer}}

Latest revision as of 02:57, 22 October 2009

Contents

Assembly of BactoMotor

Introduction

Our BactoMotor comprises of three devices –Micro-Motor, Direction Controller and Speed Controller.

As their names indicate, the Micro-Motor is the core part of the BactoMotor, and it is small (micrometer scale). The Direction Controller ensures that the propelling force generated by the bacteria translates into a coordinated and directional rotation of the motor. The Speed Controller is used to control the speed of the Bacto-motor

Each device is designed, engineered and tested separately, and functional on its own. After testing and fine-tuning, the devices are assembled to form the final System BactoMotor. The end-results is a micrometer-scale BactoMotor with unidirectional rotation, its speed controllable.

Prodecures

Step 1. Installation of Micro-Motor

The Micro-Motor will be installed into a microchip container (Fig.1a, 1b) with separated chambers. Only one side of the arms of the motor is coated with Biotin (see the Directional Controller).
Figure 1a: Photos of the microchips for micro-motor installation; A: A photo showing the chambers and the valve systems; B: Valve 3 closed; C: Valves 4, 5, 6 and 7 are closed while water is injected into the channel; D: Valve 1 and 7 closed, enclosing the crystal violet solution in the lower 4 chambers; E: The photo of the chamber system
Figure 1b: A diagram showing the Micro-Motor Module installed into a chamber inside the microchip.

Step 2. Introduction of Bacteria

The Bacteria with both Directional Controller and Speed Controller Modules implemented will be introduced to the Micro-Motor (Figure 2).
Figure 2: Introduction of Bacteria into the Micro-Motor

Step 3. Action of Directional Controller

Under the effect of the Direction Controller, all bacteria will only bind onto the sides coated with biotin on each blade of the Micro-Motor.(Fig.3a, 3b)
Fig.3a: The bacteria bounded to the Micro-Motor through action of the Direction Controller Module (Plan View)
Fig.3b: A microscopic view of the Direction Controller Module at work.

Step 4. Removing Excessive Bacteria

The extra bacterial cells will be removed by medium flush. (Fig. 4)
Fig.4: Bacteria not binding to the Micro-Motor will be flushed away by medium flush.

Step 5. BactoMotor in Action

As the Bacteria swims, they will push the motor into rotation. (clockwise as demonstrated in Fig. 5a, 5b)
Fig.5a: The Directional Rotation of the Micro-Motor achieved by the Directional Controller Module
Fig.5b: An animation showing the BactoMotor in action.

Step 6. Using the Speed Controller

To utilize the Speed Controller Module, inducer aTc is added to the Microchip containing the Micro-Motor. Increase in concentration of the inducer aTc will lead to an overall increase in rotational speed of the Bacto-Motor. (Fig. 6a, 6b)
Fig.6a: On additional of 0.5 effective dose of inducer aTc
Fig.6b: On additional of 1.0 effective dose of inducer


On the other hand, dilution/chelation of the inducer will decrease the speed of the motor.

Sponsors

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