Team:HKUST/AttractantProduction

From 2009.igem.org

(Difference between revisions)
(New page: <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /> <link href="http://igem2009hkust.fileave.com/wiki/template/120...)
 
(One intermediate revision not shown)
Line 56: Line 56:
<p>
<p>
-
  In the 20th century, a large number of organic molecules have been identified as attractant towards drosophila, and many are now being produced for industrial use. Such compounds include 2-phenylethanol, eugenol, methyleugenol, isoeugenol, methylisoeugenol, ethanol, acetic acid and glucose, etc. This broad category of attractant molecules exhibit different levels of attraction for the insect.  
+
  In the 20th century, a large number of organic molecules have been identified as attractant towards drosophila, and many are now being produced for industrial use. Such compounds include 2-phenylethanol, eugenol, methyleugenol, isoeugenol, methylisoeugenol, ethanol, acetic acid and glucose, etc. This broad category of attractant molecules exhibit different levels of attraction for the insect. <br><br>
-
   In this sub-project, we are trying to use the yeast endogenous metabolic enzyme to produce a volatile insect attractant. Attractant production would be achieved by induced over-expression of an aromatic amino-transferase, the endogenous enzyme that can catalyze a reaction to yield 2-phenylethanol. This pathway will be triggered by the downstream signal from the GPCR, when FUS1 promoter is activated.
+
   In this sub-project, we are trying to use the <em>S. cerevisiae</em> endogenous metabolic enzyme to produce a volatile insect attractant. Attractant production would be achieved by induced over-expression of an aromatic amino-transferase, the endogenous enzyme in yeast that can catalyze a reaction to yield 2-phenylethanol. This pathway will be triggered by the downstream signal from the GPCR, which activates the FUS1 promoter.
<br>
<br>

Latest revision as of 20:31, 21 October 2009

Salt and Soap template

a

In the 20th century, a large number of organic molecules have been identified as attractant towards drosophila, and many are now being produced for industrial use. Such compounds include 2-phenylethanol, eugenol, methyleugenol, isoeugenol, methylisoeugenol, ethanol, acetic acid and glucose, etc. This broad category of attractant molecules exhibit different levels of attraction for the insect.

In this sub-project, we are trying to use the S. cerevisiae endogenous metabolic enzyme to produce a volatile insect attractant. Attractant production would be achieved by induced over-expression of an aromatic amino-transferase, the endogenous enzyme in yeast that can catalyze a reaction to yield 2-phenylethanol. This pathway will be triggered by the downstream signal from the GPCR, which activates the FUS1 promoter.

  • Background
  • Experimental Design
  • Parts Design
  • Experimental Results
  • Future Work
  • References
  • HKUST