Template:Imperial/09/Overview

From 2009.igem.org

(Difference between revisions)
(New page: <html> <script> function haxbackground(){ document.getElementById('cartoonmap').style.backgroundImage='url(https://static.igem.org/mediawiki/2009/4/4d/II09_MainOverview_Desaturated.png)'; }...)
 
(31 intermediate revisions not shown)
Line 22: Line 22:
}
}
-
/* Common stuff between links */
 
dl#cartoonmap dd a{
dl#cartoonmap dd a{
position:absolute;
position:absolute;
Line 33: Line 32:
/* link positions (x,y,w,h) */
/* link positions (x,y,w,h) */
-
dl#cartoonmap dd#ph a{ left:15px; bottom:165px; width:125px; height:220px; z-index:200;}
+
dl#cartoonmap dd#ph a{left:15px; bottom:165px; width:125px; height:220px; z-index:200;}
-
dl#cartoonmap dd#at a{ left:145px; bottom:165px; width:130px; height:220px; z-index:200;}
+
dl#cartoonmap dd#at a{left:145px; bottom:165px; width:130px; height:220px; z-index:200;}
-
dl#cartoonmap dd#u a{ left:283px; bottom:165px; width:130px; height:220px; z-index:200;}
+
dl#cartoonmap dd#u a{left:283px; bottom:165px; width:130px; height:220px; z-index:200;}
-
dl#cartoonmap dd#g a{ left:420px; bottom:165px; width:135px; height:220px; z-index:200;}
+
dl#cartoonmap dd#g a{left:420px; bottom:165px; width:135px; height:220px; z-index:200;}
-
dl#cartoonmap dd#p a{ left:563px; bottom:165px; width:133px; height:220px; z-index:200;}
+
dl#cartoonmap dd#p a{left:563px; bottom:165px; width:133px; height:220px; z-index:200;}
-
dl#cartoonmap dd#time a{ left:75px; bottom:18px; width:145px; height:115px; z-index:200;}
+
dl#cartoonmap dd#time a{left:75px; bottom:18px; width:145px; height:115px; z-index:200;}
-
dl#cartoonmap dd#ci a{ left:230px; bottom:18px; width:120px; height:115px; z-index:200;}
+
dl#cartoonmap dd#ci a{left:230px; bottom:18px; width:120px; height:115px; z-index:200;}
-
dl#cartoonmap dd#t5 a{ left:365px; bottom:18px; width:145px; height:115px; z-index:200;}
+
dl#cartoonmap dd#t5 a{left:365px; bottom:18px; width:145px; height:115px; z-index:200;}
/* link hover doings */
/* link hover doings */
Line 68: Line 67:
dl#cartoonmap dd#t5 a:hover span div.first {background:pink; color:#333;}
dl#cartoonmap dd#t5 a:hover span div.first {background:pink; color:#333;}
-
dl#cartoonmap dd#ph a:hover{ background-position:-16px -33px; }
+
dl#cartoonmap dd#ph a:hover{background-position:-16px -33px;}
-
dl#cartoonmap dd#at a:hover{ background-position:-146px -33px; }
+
dl#cartoonmap dd#at a:hover{background-position:-146px -33px;}
-
dl#cartoonmap dd#u a:hover{ background-position:-284px -33px; }
+
dl#cartoonmap dd#u a:hover{background-position:-284px -33px;}
-
dl#cartoonmap dd#g a:hover{ background-position:-419px -33px; }
+
dl#cartoonmap dd#g a:hover{background-position:-419px -33px;}
-
dl#cartoonmap dd#p a:hover{ background-position:-564px -33px; }
+
dl#cartoonmap dd#p a:hover{background-position:-564px -33px;}
-
dl#cartoonmap dd#time a:hover{ background-position:-78px -283px; }
+
dl#cartoonmap dd#time a:hover{background-position:-78px -283px;}
-
dl#cartoonmap dd#ci a:hover{ background-position:-233px -283px; }
+
dl#cartoonmap dd#ci a:hover{background-position:-233px -283px;}
-
dl#cartoonmap dd#t5 a:hover{ background-position:-366px -283px; }
+
dl#cartoonmap dd#t5 a:hover{background-position:-366px -283px;}
dl#cartoonmap dd a:hover span{
dl#cartoonmap dd a:hover span{
Line 97: Line 96:
font-size:12pt;
font-size:12pt;
font-face:Arial;
font-face:Arial;
-
         padding:20px;
+
         padding:15px;
}
}
dl#cartoonmap dd#ph a:hover span{
dl#cartoonmap dd#ph a:hover span{
-
left:250px;
+
left:50px;
-
top:-250px;
+
top:100px;
}
}
dl#cartoonmap dd#at a:hover span{
dl#cartoonmap dd#at a:hover span{
-
left:120px;
+
left:50px;
-
top:-250px;
+
top:100px;
}
}
dl#cartoonmap dd#u a:hover span{
dl#cartoonmap dd#u a:hover span{
-
left:-18px;
+
left:50px;
-
top:-250px;
+
top:100px;
}
}
dl#cartoonmap dd#g a:hover span{
dl#cartoonmap dd#g a:hover span{
-
left:-155px;
+
left:-100px;
-
top:-250px;
+
top:100px;
}
}
dl#cartoonmap dd#p a:hover span{
dl#cartoonmap dd#p a:hover span{
-
left:-298px;
+
left:-250px;
-
top:-250px;
+
top:100px;
}
}
dl#cartoonmap dd#time a:hover span{
dl#cartoonmap dd#time a:hover span{
-
left:190px;
+
left:50px;
-
top:-490px;
+
top:-250px;
}
}
dl#cartoonmap dd#ci a:hover span{
dl#cartoonmap dd#ci a:hover span{
-
left:35px;
+
left:50px;
-
top:-490px;
+
top:-250px;
}
}
dl#cartoonmap dd#t5 a:hover span{
dl#cartoonmap dd#t5 a:hover span{
-
left:-100px;
+
left:50px;
-
top:-490px;
+
top:-250px;
}
}
Line 138: Line 137:
<dd id="ph"><a href="https://2009.igem.org/Team:Imperial_College_London/Chemoinduction" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
<dd id="ph"><a href="https://2009.igem.org/Team:Imperial_College_London/Chemoinduction" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
<span><div class="first">Growth</div>
<span><div class="first">Growth</div>
-
<div class="rest">We are growing the cells to a desired OD of 0.7 in order to ensure that there are enough cells to produce a wanted amount of proteins</div></span>
+
<div class="rest">The cells are grown to a critical cell density, before the system is started. It allows the culture to reach a sufficient cell number before the the cells are triggered to begin protein production. This is because protein production can slow cell growth. </div></span>
</a></dd>
</a></dd>
Line 144: Line 143:
<dd id="at"><a href="https://2009.igem.org/Team:Imperial_College_London/M1" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
<dd id="at"><a href="https://2009.igem.org/Team:Imperial_College_London/M1" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
<span><div class="first">Module 1: Protein Production</div>
<span><div class="first">Module 1: Protein Production</div>
-
<div class="rest">The first module is induced with IPTG and triggers the production of the protein of interest. As part of this project we have looked into two proteins and a peptide of interest. </div></span>
+
<div class="rest">The first module is induced with IPTG, which triggers the production of the protein of interest. As part of this project we have looked into two proteins and a peptide of interest. </div></span>
</a></dd>
</a></dd>
-
<dt>Module 2: </dt>
+
<dt>Module 2: Encapsulation</dt>
<dd id="u"><a href="https://2009.igem.org/Team:Imperial_College_London/M2" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
<dd id="u"><a href="https://2009.igem.org/Team:Imperial_College_London/M2" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
-
<span><div class="first">Clearance of Urea</div>
+
<span><div class="first">Module 2: Encapsulation</div>
-
<div class="rest">A Urea clearance device.</div></span>
+
<div class="rest">The second module is where the cell, after having produced the peptide of interest, produces colanic acid. This creates a protecting layer around teh bacterium to shelter it from the acidity of the stomach.</div></span>
</a></dd>
</a></dd>
-
<dt>Guanidine</dt>
+
<dt>Module 3: Genome deletion</dt>
<dd id="g"><a href="https://2009.igem.org/Team:Imperial_College_London/M3" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
<dd id="g"><a href="https://2009.igem.org/Team:Imperial_College_London/M3" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
-
<span><div class="first">Clearance of Guanidine</div>
+
<span><div class="first">Module 3: Genome deletion</div>
-
<div class="rest">The device managing the clearance of guanidino compounds.</div></span>
+
<div class="rest">Module 3 occurs after encapsulation of the cell containing the produced peptide of interest. This module makes the bacterium non-viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The cell is thus unable to produce any proteins and therefore unable to survive.</div></span>
</a></dd>
</a></dd>
-
<dt>Phosphate</dt>
+
<dt>Secondary Encapsulation</dt>
-
<dd id="p"><a href="./Team:NYMU-Taipei/Project/Phosphate" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
+
<dd id="p"><a href="https://2009.igem.org/Team:Imperial_College_London/Manufacturing_Considerations" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
-
<span><div class="first">Clearance of Phosphate</div>
+
<span><div class="first">Secondary Encapsulation</div>
-
<div class="rest">A Phosphate clearance and balance device.</div></span>
+
<div class="rest"> Several manufacturing considerations regarding the post-processing of the culture have been investigated. Post-processing of the culture allows the polypeptide filled cells to be converted into a pharmaceutical tablet, that can be taken orally. </div></span>
</a></dd>
</a></dd>
-
<dt>Time Regulation</dt>
+
<dt>Chemoinduction</dt>
-
<dd id="time"><a href="./Team:NYMU-Taipei/Project/Time_Regulation" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
+
<dd id="time"><a href="https://2009.igem.org/Team:Imperial_College_London/Chemoinduction" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
-
<span><div class="first">Time Regulation</div>
+
<span><div class="first">Module Integration: Chemoinduction</div>
-
<div class="rest">Using oscillators to measure time, it enables detachment from the small intestine after a certain amount of time.</div></span>
+
<div class="rest"> <b>Module 1</b> is induced by the addition of a compound, IPTG. This allows the user to 'kickstart' the system once the culture has reached a sufficiently high cell density.</div></span>
</a></dd>
</a></dd>
         <dt>Autoinduction</dt>
         <dt>Autoinduction</dt>
-
<dd id="ci"><a href="./Team:NYMU-Taipei/Project/Time_Regulation" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
+
<dd id="ci"><a href="https://2009.igem.org/Team:Imperial_College_London/Autoinduction" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
-
<span><div class="first">Time Regulation</div>
+
<span style="height:235px"><div class="first">Module Integration: Autoinduction</div>
-
<div class="rest">Using oscillators to measure time, it enables detachment from the small intestine after a certain amount of time.</div></span>
+
<div class="rest"><b>Module 2</b> is triggered by a switch from glucose consumption to a secondary carbon source consumption. When the initial preferential carbon source (glucose) is exhausted, the system will metabolise the secondary carbon source that is available. This switch triggers the promoter that controls the start of <b>Module 2</b>. By knowing the initial concentrations of each carbon source, this acts as a programmable time delay system for the activation of encapsulation.</div></span>
</a></dd>
</a></dd>
-
         <dt>Another</dt>
+
         <dt>Thermoinduction</dt>
-
<dd id="t5"><a href="./Team:NYMU-Taipei/Project/Time_Regulation" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
+
<dd id="t5"><a href="https://2009.igem.org/Team:Imperial_College_London/Thermoinduction" onmouseover="haxbackground();" onmouseout="unhaxbackground();">
-
<span><div class="first">Time Regulation</div>
+
<span><div class="first">Module Integration: Thermoinduction</div>
-
<div class="rest">Using oscillators to measure time, it enables detachment from the small intestine after a certain amount of time.</div></span>
+
<div class="rest"><b>Module 3</b> is initiated upon an increase in temperature. The system is initially grown at 28°C, at which point <b>Module 3</b> is repressed. When the temperature is raised to 42°C, this repression is blocked, triggering the start of <b>Module 3</b>. This temperature sensitive system was chosen as after encapsulation, chemical induction may be less effective due to limited diffusion.</div></span>
</a></dd>
</a></dd>
</dl>
</dl>
</html>
</html>

Latest revision as of 02:49, 22 October 2009

Growth
Growth
The cells are grown to a critical cell density, before the system is started. It allows the culture to reach a sufficient cell number before the the cells are triggered to begin protein production. This is because protein production can slow cell growth.
Module 1: Protein Production
Module 1: Protein Production
The first module is induced with IPTG, which triggers the production of the protein of interest. As part of this project we have looked into two proteins and a peptide of interest.
Module 2: Encapsulation
Module 2: Encapsulation
The second module is where the cell, after having produced the peptide of interest, produces colanic acid. This creates a protecting layer around teh bacterium to shelter it from the acidity of the stomach.
Module 3: Genome deletion
Module 3: Genome deletion
Module 3 occurs after encapsulation of the cell containing the produced peptide of interest. This module makes the bacterium non-viable. It does so by over-expressing restriction enzymes which subsequently cleave the genomic DNA into small fragments. The cell is thus unable to produce any proteins and therefore unable to survive.
Secondary Encapsulation
Secondary Encapsulation
Several manufacturing considerations regarding the post-processing of the culture have been investigated. Post-processing of the culture allows the polypeptide filled cells to be converted into a pharmaceutical tablet, that can be taken orally.
Chemoinduction
Module Integration: Chemoinduction
Module 1 is induced by the addition of a compound, IPTG. This allows the user to 'kickstart' the system once the culture has reached a sufficiently high cell density.
Autoinduction
Module Integration: Autoinduction
Module 2 is triggered by a switch from glucose consumption to a secondary carbon source consumption. When the initial preferential carbon source (glucose) is exhausted, the system will metabolise the secondary carbon source that is available. This switch triggers the promoter that controls the start of Module 2. By knowing the initial concentrations of each carbon source, this acts as a programmable time delay system for the activation of encapsulation.
Thermoinduction
Module Integration: Thermoinduction
Module 3 is initiated upon an increase in temperature. The system is initially grown at 28°C, at which point Module 3 is repressed. When the temperature is raised to 42°C, this repression is blocked, triggering the start of Module 3. This temperature sensitive system was chosen as after encapsulation, chemical induction may be less effective due to limited diffusion.

Retrieved from "http://2009.igem.org/Template:Imperial/09/Overview"