Team:Chiba/Project/medium

From 2009.igem.org

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まず、次の４種類の培地を作成した。
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First, we made 4 styles of medium.
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1)agar 1% and 薄い(約1.5 mm)
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2)agar 5% and 厚い(約7 mm)
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3)agar 1% and 薄い(約1.5 mm)
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4)agar 5% and 厚い(約7 mm)
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この４種類の培地上にNitro Cellulose filterをのせ、その上でそれぞれ13種のMutantsを3 coloniesずつ培養した。
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培養後、Nitro Cellulose filterごとそれぞれの培地をカッターで切り出して同じ厚さの2000 nMのAHLを含む培地にのせて目視で蛍光の観察を始めた。
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</tr>
</tr>
<tr>
<tr>
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<td>培地の厚さ [mm]</td>
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<td>Thickness [mm]</td>
<td></td>
<td></td>
<td>1.5</td>
<td>1.5</td>
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We put Nitro Cellulose filter on these 4 styles of medium and cultured 13 of mutants LuxR each 3 colonies.
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After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.
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*Results
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この実験をした結果、各mutant LuxRが光りだす時刻の差は、(4)のagar 5% and 厚い 培地で最も大きい差が見られた。
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<gallery>
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Image:Chiba_10mL1%25mts-GFPuv1625.jpg|1
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Image:Chiba_40mL1%25mts-GFPuv1625.jpg|2
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Image:Chiba_10mL5%25mts-GFPuv1625.jpg|3
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Image:Chiba_40mL5%25mts-GFPuv1625.jpg|4
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</gallery>
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つまり厚さが薄かったりagar が少なかったりした培地上でのmutant LuxRが光りだす時刻のばらつき具合よりも、この(4)の培地上でのばらつき具合の方がはっきりしていた。
 
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The No.4 medium afforded the most delayed time among the LuxR mutants.
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だから私たちはこの培地条件を使ってdemoをすることにした。
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Namely, the thin medium of agar was better for our animation than the thick medium.

Latest revision as of 00:38, 22 October 2009

First, we made 4 styles of medium.


No. 1 2 3 4
agar [%] 1 1 5 5
Thickness [mm] 1.5 7.0 1.5 7.0


We put Nitro Cellulose filter on these 4 styles of medium and cultured 13 of mutants LuxR each 3 colonies.


After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.


  • Results

1

2

3

4


The No.4 medium afforded the most delayed time among the LuxR mutants.

Namely, the thin medium of agar was better for our animation than the thick medium.

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