Team:EPF-Lausanne/Future directions

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----
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<font size="6" color="#007CBC"><i>Possible Applications</i></font>  
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<font size="12" color="#007CBC">Future directions</font>  
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==Possible Applications==
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<br>If we focus on the applications in industry:
<br>If we focus on the applications in industry:
* '''Bioreactors''', used in biochemical engineering. Currently, one main issue is that molecules added in bioreactors to activate synthesis of a particular protein cannot be removed once in the medium, or only very tediously, involving long and expensive filtration procedures. A major advantage of our system is that it is easily reversible: just switch the light on or off! And something as simple as a light bulbe in the reactor could control that! No need to inocculate a chemical with the risk to contaminate your bioreactor.
* '''Bioreactors''', used in biochemical engineering. Currently, one main issue is that molecules added in bioreactors to activate synthesis of a particular protein cannot be removed once in the medium, or only very tediously, involving long and expensive filtration procedures. A major advantage of our system is that it is easily reversible: just switch the light on or off! And something as simple as a light bulbe in the reactor could control that! No need to inocculate a chemical with the risk to contaminate your bioreactor.
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* '''in academic research''' : If we look further into the future, the light switch could be applied to larger model organisms (not only single cells) for example to switch genes on or off in a particular area of a tissue (the "off" state would be analogous to the case where you knock-out the gene). It would be more efficient than the techniques currently used (example: the Cre-Lox system) because of the advantages listed above, namely it would allow a fine control over the target gene, a reversible action, and above all an immediate response.
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==Future Experiments==
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* Test the entire system in '''tryptophan repressor knockout E.coli strains''': this would eliminate the interferences with the tryptophan present in the medium.
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* Test the new '''mutated version of LovTap''': according to the [https://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Results/Mutations#ILE427_to_PHE Modeling results], this version should be much more efficient and stable than the original one.
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* Try to '''mutate the sequence of the Trp promoter''' in order to have a '''higher binding affinity''' of LovTap to the DNA.
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* '''in academic research''' : If we look further into the future, the light switch could be applied to larger model organisms (not only single cells) for example to switch genes on or off in a particular area of a tissue (the "off" state would be analogous to the case where you knock-out the gene). It would be more efficient than the techniques currently used (example: the Cre-Lox system) because of the advantages listed above, namely it would allow a fine control over the target gene, a reversible action, and above all an immediate response.
 
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Latest revision as of 16:55, 21 October 2009



Contents







                                               


Future directions



Possible Applications

Our system could be very useful for industry as well as for academic research, as a new tool for regulating gene expression.
If we focus on the applications in industry:

  • Bioreactors, used in biochemical engineering. Currently, one main issue is that molecules added in bioreactors to activate synthesis of a particular protein cannot be removed once in the medium, or only very tediously, involving long and expensive filtration procedures. A major advantage of our system is that it is easily reversible: just switch the light on or off! And something as simple as a light bulbe in the reactor could control that! No need to inocculate a chemical with the risk to contaminate your bioreactor.
  • in academic research : If we look further into the future, the light switch could be applied to larger model organisms (not only single cells) for example to switch genes on or off in a particular area of a tissue (the "off" state would be analogous to the case where you knock-out the gene). It would be more efficient than the techniques currently used (example: the Cre-Lox system) because of the advantages listed above, namely it would allow a fine control over the target gene, a reversible action, and above all an immediate response.

Future Experiments

  • Test the entire system in tryptophan repressor knockout E.coli strains: this would eliminate the interferences with the tryptophan present in the medium.
  • Test the new mutated version of LovTap: according to the Modeling results, this version should be much more efficient and stable than the original one.
  • Try to mutate the sequence of the Trp promoter in order to have a higher binding affinity of LovTap to the DNA.





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