Team:IPN-UNAM-Mexico/Notebook

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As summer proyect our experimental work began at 07 - july - 2009 using 2008 bioparts catalog, unfurtunly it dosen’t work properly, we had to much troubles to take out DNA and transform, E. Coli for this we had to delay and request 2009 catalog.
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=NOTEBOOK=
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Our proyect uses 29 bioparts, 12 ligations and diferent strategys to make it functional as follow:
 
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<h1> [[Image:Month-icon.png | 50px]] June</h1>
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As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into ''E. coli''. For this reason we had to delay lab work and request the 2009 catalog.
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<h1>[[Image:Month-icon.png | 50px]] July </h1>
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<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Protocols|Protocols]]</h1>
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07-July-2009
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/July|July]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/August|August]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/September|September]]</h2>
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<h2>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Notebook/October|October]]</h2>
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We digest the follow biobriks with ECORI (E), SPEI (S), and XBAI (X).
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<h1>[[Image:Month-icon.png | 50px]][[Team:IPN-UNAM-Mexico/Safety|Safety]]</h1>
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Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is  described below:
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{| border="0.5"
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! Biobrick  
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<center>
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! Enzime restriction
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{| border="2"
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!Number
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!Biobrick
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!Restriction
|-
|-
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|R0079
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|2
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|E, S
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|BBa_R0079
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|S & P
|-
|-
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|F1610
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|3
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|E, X
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|BBa_F1610
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|X & P
|-
|-
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|B0034
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|4
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|E, S
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|BBa_K091146
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|S & P
|-
|-
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|C0078
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|5
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|E, X
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|BBa_K093005
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|X &
|-
|-
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|C0079
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|6
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|E, X
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|BBa_K081016
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|X & P
|-
|-
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|B0015
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|7
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|E, X
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|BBa_EC840
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|}
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|X & P
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-
 
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We propose this restrictions for standar assembly for the digest we use a 17 hours incubation at 37º camera.
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Digest Mix:
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{| border="0.5"
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! ERCO RI - SPEI
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! Per reaction
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|-
|-
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|Plasmidic DNA
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|8
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|align="right" | 3μl
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|BBa_K081009
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|X & P
|-
|-
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|Enzima ECORI
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|9
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|align="right" | 2μl
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|BBa_R0051
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|S &P
|-
|-
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|Enzima SPEI
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|10
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|align="right" | 2μl
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|BBa_J06800
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|X & P
|-
|-
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|Buffer NBE
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|11
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|align="right" | 2μl
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|BBa_Q04121
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|X & P
|-
|-
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|BSA
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|12
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|align="right" | 0.5μl
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|BBa_B0034
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|S & P
|-
|-
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|H2O
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|13
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|align="right" | 10.5μl
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|BBa_C0079
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|E & S
|-
|-
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!Total
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|14
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|align="right" | 20μl
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|BBa_C0179
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|E & S
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|-
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|15
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|BBa_B0015
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|E & X
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|-
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|16
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|BBa_K081018
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|E & S
 +
|-
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|17
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|BBa_K116640
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|X & P
 +
|-
 +
|23
 +
|BBa_S03154
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|X & P
 +
|-
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|24
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|BBa_k145201
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|E & S
|}
|}
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</center>
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{| border="0.5"
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<center>
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! ECORI - XBAI
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{| border="2"
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!Per reaction
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! Ligation
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! Nickname
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! Biobrick
|-
|-
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|Plasmidic DNA
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|4-23
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|align="right" | 3μl
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|Ciencias I
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|BBa_K226005
|-
|-
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|Enzima ECORI
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|13-15
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|align="right" | 2μl
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|Ciencias II
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|BBa_K266002
|-
|-
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|Enzima XBAI
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|Ciencias I-7
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|align="right" | 2μl
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|Ciencias III
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|BBa_K266006
|-
|-
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|Buffer NBE
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|24-17
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|align="right" | 2μl
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|Ciencias IV
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|BBa_K266001
|-
|-
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|BSA
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|2-3
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|align="right" | 0.5μl
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|Ciencias V
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|BBa_K266000
|-
|-
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|H20
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|J231000-11
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|align="right" | 10.5μl
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|Ciencias VI
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|BBa_K266008
|-
|-
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!Total
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|Amplicon - Ciencias II
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|align="right" | 20μl
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|Ciencias VII
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|}
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|BBa_K266003
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|-
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|Ciencias VI - 12
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<h1>[[Image:Month-icon.png | 50px]] August </h1>
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|Ciencias VIII
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|BBa_K266009
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<h1>[[Image:Month-icon.png | 50px]] September </h1>
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|-
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|Ciencias III - 19
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<h1>[[Image:Month-icon.png | 50px]] October </h1>
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|Ciencias IX
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|BBa_K266006
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|-
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|J23100 – Ciencias VII
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|Ciencias X
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|BBa_K266004
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|-
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|Ciencias III - Ciencias V
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|Ciencias XI
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|BBa_K266007
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|-
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|Ciencias X - Ciencias IV
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|Ciencias XII
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|BBa_K266010
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|}</center>
{{Template:IPN-UNAM-Mexico-footer}}
{{Template:IPN-UNAM-Mexico-footer}}

Latest revision as of 01:29, 22 October 2009


BannerUNAM.jpg

NOTEBOOK

As summer proyect our experimental work began at july the 7th 2009 using 2008 bioparts catalog, unfortunately it didn't work properly, so we had a lot of trouble to take out DNA and transform into E. coli. For this reason we had to delay lab work and request the 2009 catalog.

Month-icon.pngProtocols

Month-icon.pngJuly

Month-icon.pngAugust

Month-icon.pngSeptember

Month-icon.pngOctober

Month-icon.pngSafety

Our synthetic construction consists of 29 bioparts, which needed 12 ligations and different strategies to make it functional as is described below:


Number Biobrick Restriction
2 BBa_R0079 S & P
3 BBa_F1610 X & P
4 BBa_K091146 S & P
5 BBa_K093005 X &
6 BBa_K081016 X & P
7 BBa_EC840 X & P
8 BBa_K081009 X & P
9 BBa_R0051 S &P
10 BBa_J06800 X & P
11 BBa_Q04121 X & P
12 BBa_B0034 S & P
13 BBa_C0079 E & S
14 BBa_C0179 E & S
15 BBa_B0015 E & X
16 BBa_K081018 E & S
17 BBa_K116640 X & P
23 BBa_S03154 X & P
24 BBa_k145201 E & S


Ligation Nickname Biobrick
4-23 Ciencias I BBa_K226005
13-15 Ciencias II BBa_K266002
Ciencias I-7 Ciencias III BBa_K266006
24-17 Ciencias IV BBa_K266001
2-3 Ciencias V BBa_K266000
J231000-11 Ciencias VI BBa_K266008
Amplicon - Ciencias II Ciencias VII BBa_K266003
Ciencias VI - 12 Ciencias VIII BBa_K266009
Ciencias III - 19 Ciencias IX BBa_K266006
J23100 – Ciencias VII Ciencias X BBa_K266004
Ciencias III - Ciencias V Ciencias XI BBa_K266007
Ciencias X - Ciencias IV Ciencias XII BBa_K266010

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