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Team:Purdue/Parts
From 2009.igem.org
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| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px; margin-left:15px;">[[Team:Purdue|<span style="color:green;">Home</span>]]</span> |
| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px;">[[Team:Purdue/Team|<span style="color:green;">The Team</span>]]</span> |
| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px;">[[Team:Purdue/Project|<span style="color:green;">The Project</span>]]</span> |
| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px;">[[Team:Purdue/Parts|Parts Submitted to the Registry]]</span> |
| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px;">[[Team:Purdue/Modeling|<span style="color:green;">Modeling</span>]]</span> |
| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px;">[[Team:Purdue/Notebook|<span style="color:green;">Notebook</span>]]</span> |
| - | <span style="font-size: | + | <span style="font-size:16px; font-family:georgia; border:2px solid black; padding: 6px; margin: 6px;">[[Team:Purdue/Safety|<span style="color:green;">Safety</span>]]</span> |
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| - | [http://partsregistry.org/Part:BBa_K217000 | + | [[IMage:PUigem.JPG|400px|right]] |
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| + | '''''PLEASE NOTE: We have encountered a few uncontrollable circumstances with respect to our part's production. There has been a short delay in its shipment to us. We will send our part's DNA to the Registry by the end of the week. Sorry, and thanks for understanding!''''' | ||
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| + | This year, our submitted part (BBa_K217000) is a mammalian construct that constitutively produces the tat-GFP fusion protein (for details, see the [[Team:Purdue/Project|Project Page]]). The promoter of the part is a constitutive CMV (cytomegalovirus) promoter, so any mammalian cell with this plasmid will constantly produce tat-GFP. The tat-GFP will then diffuse out of the cell (due to tat's membrane-crossing characteristics). In essence, the cell will shoot out little green "bullets". | ||
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| + | Because tat has infectious properties (it can cross cellular membranes with ease), tat-GFP automatically causes this part to be at a Biosafety Level 2, and so should be handled with care. For more details see the [[Team:Purdue/Safety|Safety Page]]. | ||
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| + | Click here to see our part page in the Registry of Standard Biological Parts: [http://partsregistry.org/Part:BBa_K217000 Part BBa_K217000.] | ||
Latest revision as of 02:05, 22 October 2009
Home The Team The Project Parts Submitted to the Registry Modeling Notebook Safety
PLEASE NOTE: We have encountered a few uncontrollable circumstances with respect to our part's production. There has been a short delay in its shipment to us. We will send our part's DNA to the Registry by the end of the week. Sorry, and thanks for understanding!
This year, our submitted part (BBa_K217000) is a mammalian construct that constitutively produces the tat-GFP fusion protein (for details, see the Project Page). The promoter of the part is a constitutive CMV (cytomegalovirus) promoter, so any mammalian cell with this plasmid will constantly produce tat-GFP. The tat-GFP will then diffuse out of the cell (due to tat's membrane-crossing characteristics). In essence, the cell will shoot out little green "bullets".
Because tat has infectious properties (it can cross cellular membranes with ease), tat-GFP automatically causes this part to be at a Biosafety Level 2, and so should be handled with care. For more details see the Safety Page.
Click here to see our part page in the Registry of Standard Biological Parts: [http://partsregistry.org/Part:BBa_K217000 Part BBa_K217000.]
