Team:Virginia/Idea

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=Idea=
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[[Image:Idea.png|200px]] [[Image:Spongeman.PNG]]
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=Biosequestration=
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The use of microbial species for environmental remediation of heavy metals has been extensively studied. They can be used to sequester, precipitate or modify heavy metals to detoxify them. Two major inorganic cations of arsenic are both toxic to varying degrees. (See  [[Team:Virginia/Problem#Toxicity|''Toxicity'']]).
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(Intro about biosequestration)
 
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Surveying evolved strategies for arsenic detoxification in bacteria shows that the most common mechanisms involve the efflux of arsenic ions. If we're trying to biosequester arsenic then efflux mechanisms will work counter to our goal. However, storage of free arsenic ions in a cell will be toxic. Therefore a successful arsenic sponge needs to incorporate both a mechanism to prevent efflux as well as binding arsenic in a non-toxic form.
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=Storage and binding=
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Surveying evolved strategies for arsenic detoxification in bacteria shows that the most common mechanisms involve the efflux of arsenic ions. Our goal is to sequester arsenic, therefore to use a microbial chassis we must inhibit its efflux mechanism. However, storage of free arsenic ions in a cell will be toxic. A successful biosequestration strategy needs to also incorporate a binding mechanism that allows the cell to accumulate significant amounts of arsenic.  
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''Escherichia coli'' uses the [http://ecoliwiki.net/colipedia/index.php/arsB ArsB] ion transporter
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=Arsenic Sponge=
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This engineered strain of ''E. coli'' will absorb arsenic and sequester it, safely removing it from the surrounding environment. This will be accomplished by making it both deficient for the arsenic efflux pump gene and also incorporating an arsenic binding mechanism using metallothioneins. This class of cysteine rich peptides can bind arsenic, as well as other heavy metals, preventing it from interfering with cellular function.

Latest revision as of 02:43, 22 October 2009

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Idea.png Spongeman.PNG

Biosequestration

The use of microbial species for environmental remediation of heavy metals has been extensively studied. They can be used to sequester, precipitate or modify heavy metals to detoxify them. Two major inorganic cations of arsenic are both toxic to varying degrees. (See Toxicity).


Storage and binding

Surveying evolved strategies for arsenic detoxification in bacteria shows that the most common mechanisms involve the efflux of arsenic ions. Our goal is to sequester arsenic, therefore to use a microbial chassis we must inhibit its efflux mechanism. However, storage of free arsenic ions in a cell will be toxic. A successful biosequestration strategy needs to also incorporate a binding mechanism that allows the cell to accumulate significant amounts of arsenic.


Arsenic Sponge

This engineered strain of E. coli will absorb arsenic and sequester it, safely removing it from the surrounding environment. This will be accomplished by making it both deficient for the arsenic efflux pump gene and also incorporating an arsenic binding mechanism using metallothioneins. This class of cysteine rich peptides can bind arsenic, as well as other heavy metals, preventing it from interfering with cellular function.