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Team:Chiba/Project/reporter
From 2009.igem.org
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| - | === | + | ===Time delay depending on the reporters=== |
| + | In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters. | ||
| + | |||
| + | <table width="502" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr> | ||
| + | <td width="250">Reporter</td> | ||
| + | <td width="2"></td> | ||
| + | <td width="50">GFPuv</td> | ||
| + | <td width="50">mRFP</td> | ||
| + | <td width="50">sfGFP</td> | ||
| + | <td width="50">mCherry</td> | ||
| + | <td width="50">mOrange</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Time of Recognizing the fastest LuxR [h]</td> | ||
| + | <td></td> | ||
| + | <td>2</td> | ||
| + | <td>3.5</td> | ||
| + | <td>4</td> | ||
| + | <td>5</td> | ||
| + | <td>11</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Time of Recognizing the last LuxR [h]</td> | ||
| + | <td></td> | ||
| + | <td>3</td> | ||
| + | <td>24.5</td> | ||
| + | <td>50</td> | ||
| + | <td>25.5</td> | ||
| + | <td>71</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | *Condition | ||
| + | Medium ：agar 5%, Thickness 7.0 mm | ||
| + | |||
| + | Temperature : r.t. (ca. 25 degree) | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | *The plate shown in each photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation (from upper left to bottom right). | ||
| + | |||
| + | |||
| + | <gallery> | ||
| + | Image:Chiba_GFPuv2.png|GFPuv | ||
| + | Image:Chiba_MRFP2.png|mRFP | ||
| + | Image:Chiba_SfGFP2.png|sfGFP | ||
| + | Image:Chiba_MCherry2.png|mCherry | ||
| + | Image:Chiba_MOrange2.png|mOrange | ||
| + | </gallery> | ||
Latest revision as of 00:12, 22 October 2009
Time delay depending on the reporters
In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.
| Reporter | GFPuv | mRFP | sfGFP | mCherry | mOrange | |
| Time of Recognizing the fastest LuxR [h] | 2 | 3.5 | 4 | 5 | 11 | |
| Time of Recognizing the last LuxR [h] | 3 | 24.5 | 50 | 25.5 | 71 |
- Condition
Medium ：agar 5%, Thickness 7.0 mm
Temperature : r.t. (ca. 25 degree)
- The plate shown in each photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation (from upper left to bottom right).
 GFPuv |
 mRFP |
 sfGFP |
 mCherry |
 mOrange |
