Team:Osaka/RESULTS

From 2009.igem.org

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     <h2>Sensors</h2></div>  
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<p>PCR Sequencing</p>
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<h3>COLOR</h3>
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<p>&spades; Assembled 10 fluorescence protein parts for germ painting.</P>
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<p>&spades; Devised color gradation systems and developed simulation for it.</P>
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<p>>>Detail for <a href="/Team:Osaka/COLOR">COLOR</a></p>
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<br>
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<h3>SIGNAL</h3>
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<p>&clubs; Assembled 4 sender and 4 receiver parts for cell-cell communication.</p>
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<p>&clubs; Characterization of 4&times;4 combinations of sender-receiver parts.</p>
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<p>&clubs; Modeling the cell motility and development of simulation of triangle (paper-rock-scissors) model.</p>
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<p>>>Detail for <a href="/Team:Osaka/SIGNAL">SIGNAL</a></p>
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<br>
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<h3>MOTILITY</h3>
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<p>&hearts; Construction of new parts, FliH(<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K204500">K204500</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K204502">K2045002</a>, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K204503">K2045003</a>).</p>
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<p>&hearts; Confirmation of expected effect of our parts on swarming motility and protein secretion of cell.</p>
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<p>>>Detail for <a href="/Team:Osaka/MOTILITY">MOTILITY</a></p>
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<br>
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<h3>WORKS & ETHICS</h3>
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<p style="text-indent: -1em">&diams; Created a lot of Bio Art works that has implication to biotechnology, bioethics and biomedia.</p>
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<p style="text-indent: -1em">&diams; Discussed Bio Art with respect to biomedia and bioethics in the context of synthetic biology. And proposed newfangled ethical implication between science and art.</p>
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<p style="text-indent: -1em">>>Detail for <a href="/Team:Osaka/WORKS">WORKS</a> and <a href="/Team:Osaka/ETHICS">ETHICS</a></p>
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<p>Sensor Experiment 1: Receivers vs AHL<br>
 
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Procedure:<br>
 
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1. Pre-culture cells transformed with parts to be tested in solution medium overnight.<br>
 
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2. Inoculate fresh medium with pre-cultured cells and culture till full growth.<br>
 
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3. Replace culture medium by ultracentrifuging and resuspending cells in fresh medium.<br>
 
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4. Add AHL to culture.<br>
 
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5. Measure OD and fluorescence at regular intervals.<br>
 
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[link to protocol]</p>
 
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<p>Sensor Experiment 2: Senders vs Receivers<br>
 
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Procedure:<br>
 
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1. Pre-culture cells transformed with parts to be tested in solution medium overnight.<br>
 
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2. Pipette fresh medium into microcentrifuge tubes then inoculate with cells from overnight culture.<br>
 
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3. Add AHL for the Receivers-only samples.<br>
 
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4. Culture overnight at 37 degrees Celsius in incubator.<br>
 
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5. Measure fluorescence.<br>
 
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[link to protocol]</p>
 
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    <p>Under construction</p><br>
 
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     <h2>Entertainment</h2></div>  
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    <p>Under construction</p><br>  
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<h3>iCEM</h3>
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<p>S. Nakamura & K. Ikeda suggested and planed social event <a href="https://2009.igem.org/Jamboree/Social_Event">iCEM</a> this year. Collaborating with other committee members, we will entertain your night life in Boston. Why don't you join us?</p><br>
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    <p>Under construction</p> <br>
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<h3>Synthetic Biology</h3>
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<p>We don't know whether we will participate in iGEM 2010 or not. However if you are interested in synthetic or systems biology or social event, please contact us with high motivation, intelligence, special skill, will power, meddling, and fluent communication or nominication skill.</p>
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<br>
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<h3>Bio Art</h3>
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<p>We plan to continue our bio Art work if we afford it. If you interested in Bio Art, please join us and make it.</p>
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Latest revision as of 20:23, 21 October 2009

Home of iGEMOSAKA wiki

RESULTS

Achievement

COLOR

♠ Assembled 10 fluorescence protein parts for germ painting.

♠ Devised color gradation systems and developed simulation for it.

>>Detail for COLOR


SIGNAL

♣ Assembled 4 sender and 4 receiver parts for cell-cell communication.

♣ Characterization of 4×4 combinations of sender-receiver parts.

♣ Modeling the cell motility and development of simulation of triangle (paper-rock-scissors) model.

>>Detail for SIGNAL


MOTILITY

♥ Construction of new parts, FliH(K204500, K2045002, K2045003).

♥ Confirmation of expected effect of our parts on swarming motility and protein secretion of cell.

>>Detail for MOTILITY


WORKS & ETHICS

♦ Created a lot of Bio Art works that has implication to biotechnology, bioethics and biomedia.

♦ Discussed Bio Art with respect to biomedia and bioethics in the context of synthetic biology. And proposed newfangled ethical implication between science and art.

>>Detail for WORKS and ETHICS


Entertainment

iCEM

S. Nakamura & K. Ikeda suggested and planed social event iCEM this year. Collaborating with other committee members, we will entertain your night life in Boston. Why don't you join us?


Future work

Synthetic Biology

We don't know whether we will participate in iGEM 2010 or not. However if you are interested in synthetic or systems biology or social event, please contact us with high motivation, intelligence, special skill, will power, meddling, and fluent communication or nominication skill.


Bio Art

We plan to continue our bio Art work if we afford it. If you interested in Bio Art, please join us and make it.