"
Team:ArtScienceBangalore/Parts
From 2009.igem.org
(Difference between revisions)
| (17 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | |||
<html> | <html> | ||
<link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /> | <link rel="stylesheet" href="http://hackteria.org/as.css" type="text/css" /> | ||
<div id="asMain"> | <div id="asMain"> | ||
<div id="asTitle"> | <div id="asTitle"> | ||
| - | <h1> | + | <h1>Parts</h1> |
</div> | </div> | ||
| Line 10: | Line 9: | ||
<ul> | <ul> | ||
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore">Home</a></li> | ||
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Team">People</a></li> | ||
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Aproach">Approach</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Aproach">Approach</a></li> | ||
| - | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project"> | + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Project">Project</a></li> |
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Notebook">Notebook</a></li> | ||
<li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li> | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Outreach">Outreach</a></li> | ||
| - | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/ | + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Experiments">Experiments</a></li> |
| - | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li> | + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Links">References</a></li> |
| - | + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Gallery">Gallery</a></li> | |
| + | <li><a href="https://2009.igem.org/Team:ArtScienceBangalore/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 22: | Line 23: | ||
<div id="asContent"> | <div id="asContent"> | ||
| - | |||
| - | |||
| - | Here is the part that we submitted to the | + | <strong>Here is the part that we submitted to the Standard Parts Registry- <br> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000"><strong>BBa_K221000</strong></a></strong><br><br> |
| - | This helps our construct | + | <strong><i>This helps our construct synthesize geosmin, the enzyme that smells like rain.</i></strong><br> |
| + | |||
| + | This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3. Expression of this domain gives a fully functional <i>germacradienol synthase</i> with steady-state catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.<br><br> | ||
| + | |||
| + | |||
| + | <strong> <b>Amino acid sequence:</b> </strong><br> | ||
| + | |||
| + | MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK<br> | ||
| + | YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM<br> | ||
| + | RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRLH<br> | ||
| + | QFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT | ||
| + | |||
| + | </div> | ||
| + | |||
| + | </div> | ||
| + | </html> | ||
Latest revision as of 21:31, 21 October 2009
Parts
Here is the part that we submitted to the Standard Parts Registry-
BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3. Expression of this domain gives a fully functional germacradienol synthase with steady-state catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence:
MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK
YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM
RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRLH
QFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3. Expression of this domain gives a fully functional germacradienol synthase with steady-state catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence:
MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLEK
YKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIEM
RRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSRLH
QFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
