Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

(Difference between revisions)
(→Wednesday, 16 September)
 
(13 intermediate revisions not shown)
Line 29: Line 29:
</html>
</html>
{{:Team:Kyoto/CiC/Notebook_tpl}}
{{:Team:Kyoto/CiC/Notebook_tpl}}
-
==0914-0918 : what to do. what to do. what to do. what to do. ==
+
==0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
{{:Team:Kyoto/Pad_start_dates}}
{{:Team:Kyoto/Pad_start_dates}}
===Monday, 21 September===
===Monday, 21 September===
Line 36: Line 36:
**Colony PCR
**Colony PCR
====Results====
====Results====
-
*消えた
+
*Binds of expected size were observed.
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
Line 43: Line 43:
*HIV-TAT-(LALAAAA)3-generator
*HIV-TAT-(LALAAAA)3-generator
**miniprep
**miniprep
-
*TIM23complex signal::EGFP generator
+
*sig-EGFP generator(HeLa)
-
**PEGFP-N3 VECTOR (EGFP generator)
+
**Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
-
====Results====
+
**hosphorylation signal sequence primer
-
*消えた
+
**annering signal sequence primer
 +
 
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
===Wednesday, 23 September===
===Wednesday, 23 September===
====To Do====
====To Do====
 +
*sig-EGFP generator
**ligation
**ligation
**transformation
**transformation
Line 58: Line 60:
{| class="table"
{| class="table"
|+conc.
|+conc.
-
!NO.!!Sample name!!conc./(ng/ul)
+
!NO.!!Sample name!!conc./(ng/ul)!!conc.(ul)
 +
|-
 +
|1||pEGFP-N3(EcoR1,Xho1)||224.3||1
|-
|-
-
|1||11(stu1)||103.2
+
|2||signal sequence||0.3||11
|}
|}
=====Transformation=====
=====Transformation=====
-
No colony was observed (090917 10:00)　
+
No colony was observed. (090917 10:00)　
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}

Latest revision as of 02:06, 22 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)

Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.

Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • sig-EGFP generator(HeLa)
    • Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
    • hosphorylation signal sequence primer
    • annering signal sequence primer

Wednesday, 23 September

To Do

  • sig-EGFP generator
    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)conc.(ul)
1pEGFP-N3(EcoR1,Xho1)224.31
2signal sequence0.311
Transformation

No colony was observed. (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0921-0925"