Team:Bologna/Parts

From 2009.igem.org

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(→Submitted Parts)
 
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<br><br>
<br><br>
-
=Added Parts=
+
=Submitted Parts=
-
 
+
These are the new parts added and whose DNAs were sent to the Registry.
{|
{|
-
|- style="background: #1560BD; text-align: center;"
+
|- style="background: #1560BD; color:white; text-align: center;"
|width=15| '''N°'''
|width=15| '''N°'''
|width=85| '''NAME'''
|width=85| '''NAME'''
|width=85| '''TYPE'''
|width=85| '''TYPE'''
-
|width=980| '''FRAGMENT'''
+
|width=980| '''DESCRIPTION'''
|width=95| '''PLASMID'''
|width=95| '''PLASMID'''
|width=50| '''LENGTH (bp)'''
|width=50| '''LENGTH (bp)'''
Line 18: Line 18:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201000 BBa_K201000]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201000 BBa_K201000]   
| Generator
| Generator
-
| LacI repressed GFP generator with BBa_K201007.
+
| LacI repressed GFP generator with BBa_K201007
-
| BBa_PSB1A2
+
-
| 986
+
-
| -
+
-
|}
+
-
<br>
+
-
 
+
-
=Submitted Parts=
+
-
 
+
-
{|
+
-
|- style="background: #1560BD; text-align: center;"
+
-
|width=15| '''N°'''
+
-
|width=85| '''IGEM CODE'''
+
-
|width=160| '''NAME'''
+
-
|width=85| '''TYPE'''
+
-
|width=980| '''FRAGMENT'''
+
-
|width=95| '''PLASMID'''
+
-
|width=50| '''LENGTH (bp)'''
+
-
|width=1| '''SEQUENCED'''
+
-
|- style="background:#99BADD; color:black; text-align: center"
+
-
| 1
+
-
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201000 BBa_K201000] 
+
-
| 1429 O2 GFP
+
-
| Generator
+
-
| GFP protein under the control of BBa_J23118 promoter, regulated by the O2 Lac natural operator.
+
| BBa_PSB1A2
| BBa_PSB1A2
| 986
| 986
Line 48: Line 24:
|- style="background:#99BADD; color:black; text-align: center"
|- style="background:#99BADD; color:black; text-align: center"
| 2
| 2
-
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201001 BBa_K201001]   
+
| [http://partsregistry.org/Part:BBa_K201001:Experience BBa_K201001]   
-
| 2547 O2 GFP
+
| Generator
| Generator
-
| GFP protein under the control of BBa_J23100 promoter, regulated by the O2 Lac natural operator.
+
| LacI repressed GFP generator with BBa_K201008
| BBa_PSB1A2
| BBa_PSB1A2
| 986
| 986
Line 58: Line 33:
| 3
| 3
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201002 BBa_K201002]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201002 BBa_K201002]   
-
| 1429 RBS LacI T
 
| Generator
| Generator
-
| LacI repressor under the control of BBa_J23118 promoter.
+
| LacI repressor constitutive supplier
| BBa_PSB1A2
| BBa_PSB1A2
| 1351
| 1351
Line 67: Line 41:
| 4
| 4
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201002 BBa_K201002]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201002 BBa_K201002]   
-
| 1429 RBS LacI T
 
| Generator
| Generator
-
| LacI repressor under the control of BBa_J23118 promoter.
+
| LacI repressor constitutive supplier
| BBa_PSB3K3
| BBa_PSB3K3
| 1351
| 1351
Line 76: Line 49:
| 5
| 5
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201003 BBa_K201003]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201003 BBa_K201003]   
-
| 1429 I13504
 
| Generator
| Generator
-
| Wild type GFP protein under the control of BBa_J23118 promoter.
+
| GFP generator
| BBa_PSB1A2
| BBa_PSB1A2
| 918
| 918
Line 85: Line 57:
| 6
| 6
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201003 BBa_K201003]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201003 BBa_K201003]   
-
| 1429 I13504
 
| Generator
| Generator
-
| Wild type GFP protein under the control of BBa_J23118 promoter.
+
| GFP generator
| BBa_PSB3K3
| BBa_PSB3K3
| 918
| 918
Line 94: Line 65:
| 7
| 7
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201004 BBa_K201004]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201004 BBa_K201004]   
-
| O2 RFP
 
| Regulatory
| Regulatory
-
| Lac natural operator O2 with RFP.
+
| Lac natural operator O2 with RFP
| BBa_PSB1A2
| BBa_PSB1A2
| 733
| 733
Line 103: Line 73:
| 8
| 8
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201005 BBa_K201005]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201005 BBa_K201005]   
-
| O1 RFP
 
| Regulatory
| Regulatory
| Lac natural operator O1 with RFP.
| Lac natural operator O1 with RFP.
Line 112: Line 81:
| 9
| 9
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201006 BBa_K201006]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201006 BBa_K201006]   
-
| Oid RFP
 
| Regulatory
| Regulatory
-
| Lac natural operator Oid with RFP.
+
| Lac natural operator Osym with RFP.
| BBa_PSB1A2
| BBa_PSB1A2
| 732
| 732
Line 121: Line 89:
| 10
| 10
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201007 BBa_K201007]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201007 BBa_K201007]   
-
| 1429 O2 RFP
 
| Regulatory
| Regulatory
-
| Constitutive promoter BBa_J23118 regulated by LacI.
+
| LacI repressed promoter BBa_J23118
| BBa_PSB1A2
| BBa_PSB1A2
| 776
| 776
Line 130: Line 97:
| 11
| 11
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201008 BBa_K201008]   
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201008 BBa_K201008]   
-
| 2547 O2 RFP
 
| Regulatory
| Regulatory
-
| Constitutive promoter BBa_J23100 regulated by LacI .
+
| LacI repressed promoter BBa_J23100
| BBa_PSB1A2
| BBa_PSB1A2
| 776
| 776
Line 141: Line 107:
=Improved Parts=
=Improved Parts=
-
 
+
We added new information about parts already present in the Registry. More details in the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K079031 wet-lab section].
{|
{|
-
|- style="background: #1560BD; text-align: center;"
+
|- style="background: #1560BD; color:white; text-align: center;"
|width=15| '''N°'''
|width=15| '''N°'''
-
|width=85| '''IGEM CODE'''
+
|width=85| '''NAME'''
-
|width=160| '''NAME'''
+
|width=85| '''TYPE'''
|width=85| '''TYPE'''
-
|width=980| '''FRAGMENT'''
+
|width=980| '''DESCRIPTION'''
-
|width=95| '''PLASMID'''
+
|width=50| '''LENGTH (bp)'''
|width=50| '''LENGTH (bp)'''
-
|width=1| '''SEQUENCED'''
 
|- style="background:#99BADD; color:black; text-align: center"
|- style="background:#99BADD; color:black; text-align: center"
| 1
| 1
-
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201000 BBa_K201000]   
+
| [http://partsregistry.org/Part:BBa_K079031:Experience BBa_K079031]   
-
| 1429 O2 GFP
+
| Reporter
-
| Generator
+
| GFP reporter protein under the control of the BBa_J23118 promoter
-
| GFP protein under the control of BBa_J23118 promoter, regulated by the O2 Lac natural operator.
+
| 957
-
| BBa_PSB1A2
+
|- style="background:#99BADD; color:black; text-align: center"
-
| 986
+
| 2
-
| -
+
| [http://partsregistry.org/Part:BBa_K079032:Experience BBa_K079032] 
 +
| Reporter
 +
| GFP reporter protein under the control of the BBa_J23100 constitutive promoter
 +
| 957
|}
|}
 +
<br>
 +
=Added Parts=
 +
These parts should have been use in T-REX device, but we didn't succed in cloning them on standard plasimids.
 +
{|
 +
|- style="background: #1560BD; color:white; text-align: center;"
 +
|width=15| '''N°'''
 +
|width=85| '''NAME'''
 +
|width=85| '''TYPE'''
 +
|width=960| '''DESCRIPTION'''
 +
|width=80| '''LENGTH (bp)'''
 +
|- style="background:#99BADD; color:black; text-align: center"
 +
| 1
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201010 BBa_K201010] 
 +
| DNA
 +
| Cis-Repressing sequence, to be used for T-REX device
 +
| 105
 +
|- style="background:#99BADD; color:black; text-align: center"
 +
| 2
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201011 BBa_K201011] 
 +
| DNA
 +
| Trans-Repressor (4) sequence, to be used for T-REX device
 +
| 103
 +
|- style="background:#99BADD; color:black; text-align: center"
 +
| 3
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201012 BBa_K201012] 
 +
| DNA
 +
| Trans-Repressor (7) sequence, to be used for T-REX device
 +
| 100
 +
|}
 +
 +
<br>
[https://2009.igem.org/Team:Bologna/Parts ''Up'']
[https://2009.igem.org/Team:Bologna/Parts ''Up'']

Latest revision as of 01:21, 22 October 2009

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA



Submitted Parts

These are the new parts added and whose DNAs were sent to the Registry.

N° NAME TYPE DESCRIPTION PLASMID LENGTH (bp) SEQUENCED
1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201000 BBa_K201000] Generator LacI repressed GFP generator with BBa_K201007 BBa_PSB1A2 986 -
2 [http://partsregistry.org/Part:BBa_K201001:Experience BBa_K201001] Generator LacI repressed GFP generator with BBa_K201008 BBa_PSB1A2 986 Yes
3 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201002 BBa_K201002] Generator LacI repressor constitutive supplier BBa_PSB1A2 1351 Yes
4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201002 BBa_K201002] Generator LacI repressor constitutive supplier BBa_PSB3K3 1351 -
5 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201003 BBa_K201003] Generator GFP generator BBa_PSB1A2 918 Yes
6 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201003 BBa_K201003] Generator GFP generator BBa_PSB3K3 918 -
7 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201004 BBa_K201004] Regulatory Lac natural operator O2 with RFP BBa_PSB1A2 733 -
8 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201005 BBa_K201005] Regulatory Lac natural operator O1 with RFP. BBa_PSB1A2 733 -
9 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201006 BBa_K201006] Regulatory Lac natural operator Osym with RFP. BBa_PSB1A2 732 Yes
10 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201007 BBa_K201007] Regulatory LacI repressed promoter BBa_J23118 BBa_PSB1A2 776 -
11 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201008 BBa_K201008] Regulatory LacI repressed promoter BBa_J23100 BBa_PSB1A2 776 -


Improved Parts

We added new information about parts already present in the Registry. More details in the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K079031 wet-lab section].

N° NAME TYPE DESCRIPTION LENGTH (bp)
1 [http://partsregistry.org/Part:BBa_K079031:Experience BBa_K079031] Reporter GFP reporter protein under the control of the BBa_J23118 promoter 957
2 [http://partsregistry.org/Part:BBa_K079032:Experience BBa_K079032] Reporter GFP reporter protein under the control of the BBa_J23100 constitutive promoter 957


Added Parts

These parts should have been use in T-REX device, but we didn't succed in cloning them on standard plasimids.

N° NAME TYPE DESCRIPTION LENGTH (bp)
1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201010 BBa_K201010] DNA Cis-Repressing sequence, to be used for T-REX device 105
2 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201011 BBa_K201011] DNA Trans-Repressor (4) sequence, to be used for T-REX device 103
3 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K201012 BBa_K201012] DNA Trans-Repressor (7) sequence, to be used for T-REX device 100



Up

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