"
Duke University/4 June 2009
From 2009.igem.org
(Difference between revisions)
(→June 4, 2009) |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | |||
| - | |||
[[Team:Duke | Return ]] | [[Team:Duke | Return ]] | ||
==June 4, 2009== | ==June 4, 2009== | ||
| + | <pre> | ||
E: Gel to check Bioplastics #1-5 | E: Gel to check Bioplastics #1-5 | ||
R: All correct sized bands | R: All correct sized bands | ||
| Line 32: | Line 31: | ||
3 Termcat2 1.7 kb | 3 Termcat2 1.7 kb | ||
4 phaC 1.9 kb | 4 phaC 1.9 kb | ||
| + | |||
| + | </pre> | ||
Latest revision as of 16:29, 21 October 2009
June 4, 2009
E: Gel to check Bioplastics #1-5 R: All correct sized bands C: Use one as template for PCR E: PCR with Bioplastics #5 as template 5x HF buffer 5 ul dNTPs 2 ul forward primer 1.25 ul reverse primer 1.25 ul DNA template (plasmid) 0.5 ul Phusion DNA polymerase 0.3 ul H2O 14.7 ul 25 ul 98°C 30s 1x 98°C 15s 55°C 30s 72°C 1m 10s 35x 72°C 5m 4°C infinity 1x Fragment # Name Size/Length 1 pASKphaA 4.2 kb 2 phaB 900 bp 3 Termcat2 1.7 kb 4 phaC 1.9 kb
