Team:Chiba/Project/reporter

From 2009.igem.org

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===Reporterに依る光り始める時刻の差===
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===Time delay depending on the reporters===
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今回使用したReporterが光り始める時刻には、以下のような差がみられた。
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In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.
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<table width="502" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr>
<table width="502" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"><tr>
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</tr>
</tr>
<tr>
<tr>
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<td>一番早いLuxRが光り始めた時刻 [h]</td>
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<td>Time of Recognizing the fastest LuxR [h]</td>
<td></td>
<td></td>
<td>2</td>
<td>2</td>
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</tr>
</tr>
<tr>
<tr>
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<td>一番遅いLuxRが光り始めた時刻 [h]</td>
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<td>Time of Recognizing the last LuxR [h]</td>
<td></td>
<td></td>
<td>3</td>
<td>3</td>
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</tr>
</tr>
</table>
</table>
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*Condition
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Medium ：agar 5%, Thickness 7.0 mm
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Temperature : r.t. (ca. 25 degree)
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よって、Reporterを変えることで少なくとも4種のdelayを得ることができたと言える。
 
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*参考
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*The plate shown in each photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation (from upper left to bottom right).
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上段左から0 h, 2 h, 3.5 h, 下段にいって4 h, 5 h, 十分時間が経ったあとの蛍光の様子、です。
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<gallery>
<gallery>
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Image:Chiba_GFPuv.png|GFPuv
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Image:Chiba_GFPuv2.png|GFPuv
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Image:Chiba_MRFP.png|mRFP
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Image:Chiba_MRFP2.png|mRFP
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Image:Chiba_SfGFP.png|sfGFP
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Image:Chiba_SfGFP2.png|sfGFP
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Image:Chiba_MCherry.png|mCherry
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Image:Chiba_MCherry2.png|mCherry
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Image:Chiba_MOrange.png|mOrange
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Image:Chiba_MOrange2.png|mOrange
</gallery>
</gallery>

Latest revision as of 00:12, 22 October 2009

Time delay depending on the reporters

In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.

Reporter GFPuv mRFP sfGFP mCherry mOrange
Time of Recognizing the fastest LuxR [h] 2 3.5 4 5 11
Time of Recognizing the last LuxR [h] 3 24.5 50 25.5 71
  • Condition

Medium ：agar 5%, Thickness 7.0 mm

Temperature : r.t. (ca. 25 degree)



  • The plate shown in each photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation (from upper left to bottom right).


GFPuv

mRFP

sfGFP

mCherry

mOrange

Retrieved from "http://2009.igem.org/Team:Chiba/Project/reporter"