Team:BIOTEC Dresden/Results Vesicles

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=== Lipid Vesicles ===
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=== Vesicle formation in Microfluidic Chamber ===
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'''First approach: Microjetting'''
 
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Our initial idea was to make GUVs shooting aqueous material through a lipid bilayer using a microjetting setup (principle similar to blowing soap bubbles).  
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The video shows the formation of vesicles in a V-chamber. The surfactant Span 80 at a concentration (V/V) of 1% was dissolved in the oil phase. The flow rates used were different for every chamber used and are hence not transferable to other systems. Vesicle size varied with applied flow rates: increased flow rates of the aqueous phase yielded in larger vesicles. Above a critical flow rate the aqueous phase would displace the oil phase.
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[[Image:Our_1st_GUVs.jpg|300px]]
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Vesicle formation in V-chamber. Surfactant: 1% Span 80
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Unfortunately, this method turned to be not technically reliable and the GUVs produced were not stable time wise and had broad size distribution.
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The vesicles have been observed in the monitoring section of the microfluidic chamber. Figure 1 shows a typical vesicle moving through a channel.
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{|
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|  [[Image:vesicle_4.jpg|thumb|400|Fig. 1: Vesicles moving through microfluidic channel]]
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'''Second approach: Mixing in Microfluidic Chamber'''
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The typical diameter of the vesicles was 15 µm, the volume under the reasonable assumtion of spherical shape was around 12,5 pl. Figure 2 illustrates the narrow size distribution of the vesicles.
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A microfluidic chamber that created an intersection between aqueous and oil phases produced vesicles that were uniform in size and stable for up to 5 hours. The vesicles are stabilized by a surfactant, Span 80.
 
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{|
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|  [[Image:vesicle_2.jpg|thumb|400|Fig. 2: Multiple vesicles of near equal size]]
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In the figure below vesicles are led into a grid by funnel shaped structures in the flow chamber (top).
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The stability of the vesicles mostly exceeded 2 hours. The vesicles would fuse over night.
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[[Image:vesicle_1.jpg|500px|vesicles created in microfluid chamber]]
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The video shows first see the part of the chamber where aqueous and oil phases meet. Oil flows from left to right in the channel that appears colorless. Two channels that carry material in the aqueous phase meet (this area is not visible in the image) and lead downwards towards the oil channel. The vesicles created are lead towards a network of channels that break the vesicles into smaller vesicles and eventually into a grid that stores them.
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Latest revision as of 02:57, 22 October 2009

Vesicle formation in Microfluidic Chamber

The video shows the formation of vesicles in a V-chamber. The surfactant Span 80 at a concentration (V/V) of 1% was dissolved in the oil phase. The flow rates used were different for every chamber used and are hence not transferable to other systems. Vesicle size varied with applied flow rates: increased flow rates of the aqueous phase yielded in larger vesicles. Above a critical flow rate the aqueous phase would displace the oil phase.

Vesicle formation in V-chamber. Surfactant: 1% Span 80

The vesicles have been observed in the monitoring section of the microfluidic chamber. Figure 1 shows a typical vesicle moving through a channel.

Fig. 1: Vesicles moving through microfluidic channel

The typical diameter of the vesicles was 15 µm, the volume under the reasonable assumtion of spherical shape was around 12,5 pl. Figure 2 illustrates the narrow size distribution of the vesicles.


Fig. 2: Multiple vesicles of near equal size

The stability of the vesicles mostly exceeded 2 hours. The vesicles would fuse over night.

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