Team:Chiba/Project/for painting

From 2009.igem.org

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[[Team:Chiba/Project|back]]
=== To draw more better picture ===
=== To draw more better picture ===
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*we screened the "agar media condition" (the thickness & the agar conc.) to make difference in the delay of the LuxR mutant more bigger(appearent?).
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*we screened the "agar media condition" (the thickness & the agar conc.) to enlarge difference in the delay of the LuxR mutant.
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*ちょっと思ったのだがこのAHL濃度とか培地とかふりまくってるのを載せると，LuxRの時間差が見えた理由が「LuxR変異体のおかげ」というよりは「培地のおかげ」っぽくなるので，この培地スクリーニングは実は載せない（というかちっちゃくのせとくのは良いけどVIP待遇はしない）方がまだ恰好がつくのでは...^w^;--[[User:Maiko|Maiko]] 14:05, 20 October 2009 (UTC)
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*”より差をみせるため”の実験なのでここでは軽く説明し、あとはリンクつけます。--[[User:Yoshimi|Yoshimi]] 14:15, 20 October 2009 (UTC)
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*そもそも「培地ふった実験」は項目なくても良いかも：デモらへんの実験条件にリンクつけるとか。レポーターは載せてもいいのでは（RFPはビミョーに差でたよね確か）--[[User:Maiko|Maiko]] 14:34, 20 October 2009 (UTC)
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*培地のよりよい条件をみつける実験
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(実験方法と結果。)
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ここに結果の写真だけのせる。--[[User:Yoshimi|Yoshimi]] 14:17, 20 October 2009 (UTC)
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*Finding more useful condition of medium to demonstrate
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<gallery>
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Image:Chiba_10mL1%25mts-GFPuv1625.jpg
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Image:Chiba_40mL1%25mts-GFPuv1625.jpg
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Image:Chiba_10mL5%25mts-GFPuv1625.jpg
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Image:Chiba_40mL5%25mts-GFPuv1625.jpg
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</gallery>
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私たちはデモをよりはっきり見せるために必要な培地の条件を調べた。
 
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[[Team:Chiba/Project/medium|Best condition of medium]]
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We researched the best condition of medium to display our demonstration more clearly.
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The protocol of this experiment is [[Team:Chiba/Project/medium|here]].
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*Reporter毎の応答の差についての実験と結果。（ＧＦＰuvがＤｅｌａｙを見やすい）
 
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私たちは、ReporterとしてGFPuv, sfGFP, mRFP, mCherry, mOrangeを使って上に挙げたものと同じ実験をした。
 
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*About the difference of responce of each reporters
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First, we did noted above experiment using GFPuv as reporter.
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Also we did similar experiment using sfGFP, mRFP, mCherry, and mOrange to judge our mutant LuxR.
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その結果、GFPuvをReporterとして使った場合が最もmutant LuxR間の光り始める時刻の差をはっきりと確認できた。
 
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As a result, we found that GFPuv is better reporter for our demonstration.
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*Reporter毎の応答についての考察。
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The protocol of this experiment is
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最もmutant LuxR間の光り始める時刻の差を確認しやすかった条件は、以下の通りであった。
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Reporter : GFPuv
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medium : agar 5%
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thickness : 7 mm
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この条件でデモをやれば、各mutant LuxRの個性（遅れ方の特徴）を最大限に活かすことができると考えた。
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そうするとメリハリのあるきれいなアニメーションを描くことが出来るのではないか。
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また、使ったReporterによって光り始める時刻に差があったのでこちらにまとめます。
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[[Team:Chiba/Project/reporter|Distinction of fluorescence protein expression between reporters]]
[[Team:Chiba/Project/reporter|Distinction of fluorescence protein expression between reporters]]

Latest revision as of 00:32, 22 October 2009

back

To draw more better picture

  • we screened the "agar media condition" (the thickness & the agar conc.) to enlarge difference in the delay of the LuxR mutant.


  • Finding more useful condition of medium to demonstrate
Chiba 10mL1%mts-GFPuv1625.jpg
Chiba 40mL1%mts-GFPuv1625.jpg
Chiba 10mL5%mts-GFPuv1625.jpg
Chiba 40mL5%mts-GFPuv1625.jpg


We researched the best condition of medium to display our demonstration more clearly.

The protocol of this experiment is here.


  • About the difference of responce of each reporters

First, we did noted above experiment using GFPuv as reporter. Also we did similar experiment using sfGFP, mRFP, mCherry, and mOrange to judge our mutant LuxR.


As a result, we found that GFPuv is better reporter for our demonstration.


The protocol of this experiment is Distinction of fluorescence protein expression between reporters

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