"
Team:uOttawa/Biobricks
From 2009.igem.org
| (4 intermediate revisions not shown) | |||
| Line 29: | Line 29: | ||
</div> | </div> | ||
<h1 id="header"> | <h1 id="header"> | ||
| - | uOttawa | + | uOttawa iGEM2009</h1> |
<div id="wrap"> | <div id="wrap"> | ||
<div id="page"> | <div id="page"> | ||
| - | + | <h2>Biobricks</h2> | |
| - | + | <p> | |
| - | <h2> | + | <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=uOttawa&Done=1">Take a look at our parts</a> |
| + | </p> | ||
| + | <p> | ||
| + | <a href="http://partsregistry.org/Part:BBa_K234095">BBa_K234095 Characterization</a> | ||
| + | </p> | ||
| + | <h2>Overview</h2> | ||
<p> | <p> | ||
We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells | We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells | ||
| Line 50: | Line 55: | ||
<a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li> | <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li> | ||
<li> | <li> | ||
| + | <a href="http://www.ipm-int.org/boxmode/pdf/Safety.pdf">Project Safety</a></li> | ||
| + | <li> | ||
<a href="http://www.ipm-int.org/boxmode/pdf/business.pdf">uOttawa iGEM integrating business and | <a href="http://www.ipm-int.org/boxmode/pdf/business.pdf">uOttawa iGEM integrating business and | ||
science</a></li> | science</a></li> | ||
| Line 57: | Line 64: | ||
<li> | <li> | ||
<a href="http://www.ipm-int.org/boxmode/pdf/Glucose.pdf">Glucose and cellulose digestion</a></li> | <a href="http://www.ipm-int.org/boxmode/pdf/Glucose.pdf">Glucose and cellulose digestion</a></li> | ||
| - | + | ||
<li> <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent">Probiotics</a></li> | <li> <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent">Probiotics</a></li> | ||
<li> <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</a></li> | <li> <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</a></li> | ||
| Line 87: | Line 94: | ||
<h3>Latest Events</h3> | <h3>Latest Events</h3> | ||
<ul id="bits"> | <ul id="bits"> | ||
| + | <li>team bonding at the sushi restaurant</li> | ||
| + | <li>world congress on industrial biotechnology, Montreal</li> | ||
<li>the waterloo getogether with other ontario teams</li> | <li>the waterloo getogether with other ontario teams</li> | ||
<li>tech museum on the 5th of May</li> | <li>tech museum on the 5th of May</li> | ||
Latest revision as of 02:44, 22 October 2009
uOttawa iGEM2009
Biobricks
Overview
We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells
