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Team:Chiba/Notebook/Calendar/30 September 2009
From 2009.igem.org
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We eluted it 50 uL of dH<sub>2</sub>O. | We eluted it 50 uL of dH<sub>2</sub>O. | ||
| - | === Gel | + | === Gel electrophoresis === |
17:32 | 17:32 | ||
| - | + | Electrophoresis | |
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Took a picture | Took a picture | ||
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=== Ligation === | === Ligation === | ||
Latest revision as of 23:26, 21 October 2009
(29_September_2009 <|>1_October_2009)
Contents |
Transformation
Yesterday's operation is here.
- Today's operation
10:30-
Culture at 37 degrees Celsius
20:00-
Mini Prep.
20:40-
Digestion Test
20:56-
At 37 degrees Celsius
21:45-
Gel electro...(135 V, 25 min)
PCR
Yesterday's operation is here.
11:15-
Gel electro...(135 V, 27 min)
12:10
Took a picture
Cloning
Digestion
Yesterday's operation is here.
12:37-
Gel electro...(100 V, 40 min)
13:20
into ethyl bromide
13:35
Took a picture and cut the gel.
Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.
DNA Clean & Concentrator
We eluted it 50 uL of dH2O.
SAP treatment
DNA Clean & Concentrator
We eluted it 50 uL of dH2O.
Gel electrophoresis
17:32
Electrophoresis
18:15
Took a picture
Ligation
18:30
@Room Temperture
21:30
Transformation
21:50
@37 degrees Celsius
23:10
Plating
