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Team:Wash U/Calendar-Home/7 July 2009
From 2009.igem.org
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== Tuesday July 7, 2009 == | == Tuesday July 7, 2009 == | ||
| + | - Ran [https://static.igem.org/mediawiki/2009/f/f0/7-7_ligation_products.jpg Gel] of digestion and ligation products from Biobrick Assembly <br> | ||
| - | - Made glycerol stock solutions: <br> | + | - Performed colony PCR on colonies transformed with plasmid from ligation reaction <br> |
| + | Results: [https://static.igem.org/mediawiki/2009/b/b6/Colony_PCR_7-8.jpg Gel] [https://static.igem.org/mediawiki/2009/9/9f/8_colonies_from_colony_PCR.jpg Colonies] <br> | ||
| + | Red colonies indicate incorrect ligation <br> | ||
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| + | - Made [https://2009.igem.org/Team:Wash_U/Protocol#Glycerol_Stock_Solution glycerol stock solutions]: <br> | ||
1. pSB1AC3 <br> | 1. pSB1AC3 <br> | ||
2. puc19 E. coli <br> | 2. puc19 E. coli <br> | ||
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14. pSB1K3 <br> | 14. pSB1K3 <br> | ||
15. R. sphaeroides 2.4.1 <br> | 15. R. sphaeroides 2.4.1 <br> | ||
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| + | -Purified genomic DNA of E. coli and R. sphaeroides for PCR <br> | ||
| + | DNA Quantities: <br> | ||
| + | 1. E.coli (pellet from 1.5 mL of culture): 54.5 ng/uL <br> | ||
| + | 2. E.coli (pellet from 3.0 mL of culture): 70.0 ng/uL <br> | ||
| + | 3. R.sphaeroides (from 3.0 mL of culture): 62.5 ng/uL <br> | ||
| + | 4. R.sphaeroides (from 1.5 mL of culture): 40.5 ng/uL <br> | ||
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Latest revision as of 16:45, 9 July 2009
