"
Team:Kyoto/CiC/Parts
From 2009.igem.org
(Difference between revisions)
(→Part design) |
|||
| (5 intermediate revisions not shown) | |||
| Line 21: | Line 21: | ||
#[[Team:Kyoto/CiC|Cells in Cells]] | #[[Team:Kyoto/CiC|Cells in Cells]] | ||
#Parts | #Parts | ||
| + | {{:Team:Kyoto/topicpath}} | ||
</div><!-- /#topicpath --> | </div><!-- /#topicpath --> | ||
{{:Team:Kyoto/Leftnavi}} | {{:Team:Kyoto/Leftnavi}} | ||
| Line 28: | Line 29: | ||
==Parts== | ==Parts== | ||
===Part design=== | ===Part design=== | ||
| - | In this project, we designed two plasmids for RTS; 1. HIV-TAT::(LALAAAA)3 expressing vector, 2.Signal for TIM23 complex::GFP expressing vector, | + | In this project, we designed two plasmids for RTS(Rapid Translation System, Roache); 1. HIV-TAT::(LALAAAA)3 expressing vector, 2.Signal for TIM23 complex::GFP expressing vector, and one plasmid for HeLa cells; Signal for TIM23 complex::EGFP expressing vector for HeLa cell. Both 1 and 3 have T7 promoter, terminator and their CDS between them. They can express each protein by RTS. Both parts are inserted in high copy number plasmid pSB1A2. 3. is made by inserting Signal for TIM23 complex into pEGFP-N3. |
===HIV-TAT-(LALAAAA)3-generator=== | ===HIV-TAT-(LALAAAA)3-generator=== | ||
| Line 34: | Line 35: | ||
===TIM23complex signal::EGFP generator=== | ===TIM23complex signal::EGFP generator=== | ||
| - | [[Image: | + | [[Image:Figure(TIM23complex signal--EGFP generator).png|400px|center]] |
===Signal for TIM23 complex::EGFP expressing vector for HeLa cells=== | ===Signal for TIM23 complex::EGFP expressing vector for HeLa cells=== | ||
Latest revision as of 02:58, 22 October 2009
Parts
Part design
In this project, we designed two plasmids for RTS(Rapid Translation System, Roache); 1. HIV-TAT::(LALAAAA)3 expressing vector, 2.Signal for TIM23 complex::GFP expressing vector, and one plasmid for HeLa cells; Signal for TIM23 complex::EGFP expressing vector for HeLa cell. Both 1 and 3 have T7 promoter, terminator and their CDS between them. They can express each protein by RTS. Both parts are inserted in high copy number plasmid pSB1A2. 3. is made by inserting Signal for TIM23 complex into pEGFP-N3.
HIV-TAT-(LALAAAA)3-generator
TIM23complex signal::EGFP generator
.png)
Signal for TIM23 complex::EGFP expressing vector for HeLa cells
2.png)
