Team:LCG-UNAM-Mexico/Wet Lab/Objectives
From 2009.igem.org
(Difference between revisions)
(→Laura Gomez) |
|||
(6 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
+ | __FORCETOC__ | ||
{{Template:LCG_top_Netscape}}<!--Do not remove the first and last lines in this page!--> | {{Template:LCG_top_Netscape}}<!--Do not remove the first and last lines in this page!--> | ||
Line 9: | Line 10: | ||
=='''General objectives'''== | =='''General objectives'''== | ||
- | + | <br>The final aim is to get and test the following device. | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | The final aim is to get and test the following device. | + | |
[[Image:P4_genome.jpg]] | [[Image:P4_genome.jpg]] | ||
+ | [[Image:Delivery_sistem.png|470px]] | ||
==='''I. Biobrick Assembly of the Kamikaze system'''=== | ==='''I. Biobrick Assembly of the Kamikaze system'''=== | ||
<br> | <br> | ||
- | ''Many cuts, pastes and clones for biobrick organization into the suicide system!!<br> In the hands of: | + | ''Many cuts, pastes and clones for biobrick organization into the suicide system!!<br> In the hands of: [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Abraham Avelar|Abraham]] and [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Enrique Paz|Paz]]'' |
<br> | <br> | ||
==='''II. Construction of the standardized P4 vector'''=== | ==='''II. Construction of the standardized P4 vector'''=== | ||
<br> | <br> | ||
- | ''An incredible and challenging fight against PCR and logical thinking!!In charge: | + | ''An incredible and challenging fight against PCR and logical thinking!!<br>In charge: [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Fernando Montaño|Nando]] and [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Enrique Paz|Paz]]'' |
<br> | <br> | ||
==='''III. Construction of the P4-producing strain'''=== | ==='''III. Construction of the P4-producing strain'''=== | ||
<br> | <br> | ||
- | ''A passionate struggle with natural selection and recombination!!<br> | + | ''A passionate struggle with natural selection and recombination!!<br>Leading: |
[[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Uriel Urquiza|Uriel]], [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Laura Gómez|Laura]], and Miguel'' | [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Uriel Urquiza|Uriel]], [[Team:LCG-UNAM-Mexico/Wet_Lab/Objectives#Laura Gómez|Laura]], and Miguel'' | ||
- | <br> | + | <br > |
==='''IV. System testing and parameter obtention'''=== | ==='''IV. System testing and parameter obtention'''=== | ||
<br> | <br> | ||
Line 66: | Line 62: | ||
- | [[Team:LCG-UNAM-Mexico/LauraJournal Journal]] | + | [[Team:LCG-UNAM-Mexico/LauraJournal|Journal]] |
** To obtain all the relevant experimental information about T7 and T3. | ** To obtain all the relevant experimental information about T7 and T3. | ||
Line 75: | Line 71: | ||
** Generation of data to feedback the infection model. | ** Generation of data to feedback the infection model. | ||
- | Our [[Team:LCG-UNAM-Mexico:Modelling | Model]] Simulates both Molecular and Population Dynamics for the Defense System. Important parameters must be estimated experimentally . | + | Our [[Team:LCG-UNAM-Mexico:Modelling | Model]] Simulates both Molecular and Population Dynamics for the Defense System. Important parameters must be estimated |
- | + | experimentally. The most important parameter in the defense system is the[[Team:LCG-UNAM-Mexico:BSD| Burst Size]]. Experimental measures | |
- | + | of the Burst Size are of vital importance. The [[Team:LCG-UNAM-Mexico:Molecular model| Molecular Model]] will simulate the intracellular | |
- | + | dynamics and will generate a Burst Size Distribution. With my experimental results we will validate and improve the model. | |
- | + | At the population scale the [[Team:LCG-UNAM-Mexico:CA|Cellular Automaton]] will simulate the interaction between populations of | |
- | The [[Team:LCG-UNAM-Mexico:Molecular model| Molecular Model]] will simulate the intracellular dynamics and will generate a Burst Size Distribution. With my experimental results we will validate and improve the model. | + | bacteria and phages, T7 infection experiments will provide valuable information to the Population model. |
- | + | ||
- | + | ||
====Abraham Avelar==== | ====Abraham Avelar==== |
Latest revision as of 03:51, 22 October 2009