Team:Warsaw/Calendar-Main/6 July 2009

From 2009.igem.org

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==Informal meeting concerning important issues of the Project==
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<h3>Informal meeting concerning important issues of the Project</h3>
-
===Michał, Paweł===
+
<h4>Michał, Paweł</h4>
Today in the evening we met in the lab located on Pawinskiego street to, during some transformations, discuss problematic issues of the Project.
Today in the evening we met in the lab located on Pawinskiego street to, during some transformations, discuss problematic issues of the Project.
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<h3>Gradient PCR inv</h3>
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<h3>PCR inv</h3>
<h4>Kama</h4>
<h4>Kama</h4>
<br />
<br />
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<br />
<br />
<p>Methods:</p>
<p>Methods:</p>
-
<ul><li><p>PCR mixture's composition:</p> 1ul pfu buffer (Fermentas), 1ul MgSO4 (Fermentas), 0,5ul primers, 0,5ul dNTPs (10 mM), 0,25ul pfu turbo polymerase, 0,5ul template DNA from Listeria, optionally: 0,75ul DMSO, solution was topped up with H2O to 10ul.  
+
<ul><li><p>PCR mixture's composition:</p>  
 +
<pre>1&mu;l pfu buffer (Fermentas)
 +
1&mu;l MgSO<sub>4</sub> (Fermentas)  
 +
0,5&mu;l primers
 +
0,5&mu;l dNTPs (10 mM)
 +
0,25&mu;l pfu turbo polymerase
 +
0,5&mu;l template DNA from Listeria
 +
optionally: 0,75&mu;l DMSO</pre>
 +
solution was topped up with H<sub>2</sub>O to 10&mu;l.  
</li>
</li>
</ul>
</ul>
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<li>2-6 - samples (annealing temperature increases to from the left to the right)</li>
<li>2-6 - samples (annealing temperature increases to from the left to the right)</li>
<li>7-11 - samples with DMSO (annealing temperature increases to from the left to the right)
<li>7-11 - samples with DMSO (annealing temperature increases to from the left to the right)
-
<li>M - GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
+
<li>M - GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li></ol>
<br/>
<br/>
<p>Notes:</p>
<p>Notes:</p>
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<br />
<br />
 +
<h3>Making Llo fusion with secretion signal peptide</h3>
 +
<h4>Kuba</h4>
 +
<br />
 +
<p>Tasks:</p>
 +
<ul>
 +
<li>Amplification of llo(hly) for fusion with the secretion signal peptide</li>
 +
</ul>
 +
<br />
 +
<p>Methods:</p>
 +
<ul><li><p>PCR mixture's composition:</p>
 +
<pre>2,5ul pfu buffer (Fermentas)
 +
2,5ul MgSO<sub>4</sub> (Fermentas)
 +
1,5ul primers
 +
1,5ul dNTPs (10 mM)
 +
0,5ul pfu turbo polymerase
 +
1 ul of DMSO, 1ul template DNA from Listeria</pre>
 +
the solution was topped up with H<sub>2</sub>O to 25ul.
 +
</li>
 +
</ul>
 +
<ul>
 +
<li>PCR programs:</li>
 +
<p>hly</p><pre align="left">4min 95&deg;C <br/> (30s 95&deg;C, 40s 42-46&deg;C, 1min30s 72&deg;C)x3 <br/> (30s 95&deg;C, 40s 46-52&deg;C, 1min30s 72&deg;C)x28 <br/> 10min 72&deg;C <br/> ~ 7&deg;C</pre>
-
</html>
+
</ul>
 +
<ul>
 +
<li>Electrophoretic separation on 1% agarose gel</li>
 +
</ul>
 +
<br />
 +
<p>Results:</p>
 +
<img src="https://static.igem.org/mediawiki/2009/d/d0/Gradient3.JPG"/>
 +
<var>
 +
<ul>
 +
<li>Gel (from left)</li>
 +
</ul>
 +
<ol>
 +
<li>M - GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
 +
<li>1-7 - samples (annealing temperature increases to from the left to the right)</li>
 +
</ol>
 +
<br/>
 +
<p><b>an even smaller relative amount of specific product has been obtained - a low-temperature PCR (followed by gel extraction) or reamplification appear to be necessary</font></b></p>
 +
</var>
 +
<br />
 +
 +
 +
</html>
 +
<html>
 +
<h3>Cloning of p53 coding sequence</h3>
 +
<h4>Marcin</h4>
 +
<br/>
 +
<p>Tasks:</P>
 +
<ul>
 +
<li>Transformation of chemocompetent E. coli strain DH5alpha with ligated pKS plasmid containing p53 coding sequence.<br/><br/>Second trial</li>
 +
</ul>
 +
<br/>
 +
<p>Methods:</p><ul>
 +
<li>thaw bacteria on the ice  - 10 minuts</li>
 +
<li>add 10 ul of ligation mixture to the bacteria</li>
 +
<li>incubation on the ice - 20 minutes<li>
 +
<li>heat shock - 1 minut, 42&deg;C</li>
 +
<li>incubation on the ice - 2 minuts</li>
 +
<li>add 800 ul of SOB medium to the bacteria</li>
 +
<li>incubation in 37&deg;C - 1 h</li>
 +
<li>Plating on selective LB medium supplemented with ampicillin, X-Gal and IPTG</li></ul><br/>
 +
<b>Comment:</b>
 +
<p>Ligation was unsuccesful or there was something wrong with the plates</p>
 +
</html>
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{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 12:02, 18 September 2009


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Informal meeting concerning important issues of the Project

Michał, Paweł

Today in the evening we met in the lab located on Pawinskiego street to, during some transformations, discuss problematic issues of the Project. Here are some problems we have to solve:

  • how to locate whole system on 1-2 plasmids (I mean - how to prevent promotor leakage due to parallel arangement of operons?
    • new standard for cloning inverted parts?
    • or should we make just inverted parts - with exchanged prefix and suffix - it will be compatible with standard but in the opposite way :)


  • Where to put the secretion system genes?
    • and should it be inducible of constitutively expressed?


  • Secretion signal is a real problem if we want to direct our proteins to mitochondria. Both secretion and mito- signals are located in the N-terminus of protein
    • with p53 another problem is that we do not know if even with mito signal it will be located in mitochondria. Romek from ZGUW is a great source of knowledge :) :) :) and he said that proteins containing zinc fingers need to have nuclar export signal (NES) to be located outside nucleus


  • as it can be see from above point - p53 isn't ideal for induction of apoptosis
    • of course we can do some 'real research' and check what conditions are needed for p53 to function properly in that manner, but is it worth our precious holiday time?
      • we don't know, or do we? if p53, just produced by bacteria can work as the inducer of apoptosis - eukaryotic proteins could be modified in multiple ways, and p53 isn't the exception - it can be modified by phosporylation, ubiquitination, sumoilation and some other, with each modification changing its function
    • interesting alternative of p53 is Bax, which is also mitochondiral protein
      • it can be easily accesible from ZGUW (Romek again! :) )
      • we are sure that bax is inducing apoptosis and that it is a wonderful killer of eukaryotic cells
      • but we don't know if bax protein will be toxic for bacterial cells - it has an ability to kill Saccharomyces cerevisiae if overexpressed so in bacteria it can also occur


  • where to put LLO? in the invasion operon or in the endosome operon?
    • in my opinion (Paweł) it should be expressed in endosome - so the second operon
    • but Michał says that LLO need to have time for expression and escape from the endosome should be quick. Endosome operon is important especially for switching off the invasion operon

What do we have to do?? - solve these problems :) some literature search is needed


What's more - we have also planned some clonings

PCR inv

Kama


Tasks:

  • Amplification of inv

Methods:

  • PCR mixture's composition:

    1μl pfu buffer (Fermentas)
    1μl MgSO4 (Fermentas) 
    0,5μl primers
    0,5μl dNTPs (10 mM)
    0,25μl pfu turbo polymerase
    0,5μl template DNA from Listeria
    optionally: 0,75μl DMSO
    solution was topped up with H2O to 10μl.
  • PCR programs:
  • inv

    4min 95°C 
    (30s 95°C, 1min 45-55°C, 4min 72°C)x3
    (30s 95°C, 1min 55-60°C, 4min 72°C)x28
    10min 72°C
    ~ 7°C
  • Electrophoretic separation on 1% agarose gel

Results:

  • Gel (from left)
  1. control -
  2. 2-6 - samples (annealing temperature increases to from the left to the right)
  3. 7-11 - samples with DMSO (annealing temperature increases to from the left to the right)
  4. M - GeneRuler DNA Ladder Mix #SM0333 (Fermentas)

Notes:

  • many unspecific products were obtained

Making Llo fusion with secretion signal peptide

Kuba


Tasks:

  • Amplification of llo(hly) for fusion with the secretion signal peptide

Methods:

  • PCR mixture's composition:

    2,5ul pfu buffer (Fermentas) 
    2,5ul MgSO4 (Fermentas)
    1,5ul primers
    1,5ul dNTPs (10 mM)
    0,5ul pfu turbo polymerase
    1 ul of DMSO, 1ul template DNA from Listeria
    the solution was topped up with H2O to 25ul.
  • PCR programs:
  • hly

    4min 95°C 
    (30s 95°C, 40s 42-46°C, 1min30s 72°C)x3
    (30s 95°C, 40s 46-52°C, 1min30s 72°C)x28
    10min 72°C
    ~ 7°C
  • Electrophoretic separation on 1% agarose gel

Results:

  • Gel (from left)
  1. M - GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
  2. 1-7 - samples (annealing temperature increases to from the left to the right)

an even smaller relative amount of specific product has been obtained - a low-temperature PCR (followed by gel extraction) or reamplification appear to be necessary


Cloning of p53 coding sequence

Marcin


Tasks:

  • Transformation of chemocompetent E. coli strain DH5alpha with ligated pKS plasmid containing p53 coding sequence.

    Second trial

Methods:

  • thaw bacteria on the ice - 10 minuts
  • add 10 ul of ligation mixture to the bacteria
  • incubation on the ice - 20 minutes
  • heat shock - 1 minut, 42°C
  • incubation on the ice - 2 minuts
  • add 800 ul of SOB medium to the bacteria
  • incubation in 37°C - 1 h
  • Plating on selective LB medium supplemented with ampicillin, X-Gal and IPTG

Comment:

Ligation was unsuccesful or there was something wrong with the plates


April
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_April_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_April_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_April_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_April_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_April_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_April_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_April_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_April_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_April_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_April_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_April_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_April_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_April_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_April_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_April_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_April_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_April_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_April_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_April_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_April_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_April_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_April_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_April_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_April_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_April_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_April_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_April_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_April_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_April_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_April_2009 30]
May
MTWTFSS
        [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_May_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_May_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_May_2009 3]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/4_May_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_May_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_May_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_May_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_May_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_May_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_May_2009 10]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/11_May_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_May_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_May_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_May_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_May_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_May_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_May_2009 17]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/18_May_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_May_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_May_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_May_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_May_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_May_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_May_2009 24]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/25_May_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_May_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_May_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_May_2009 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_June_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_June_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_June_2009 28]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/29_June_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_July_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_July_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_July_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_July_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 5]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/6_July_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_July_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_July_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_July_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_July_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_July_2009 12]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_July_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_July_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_July_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_July_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_July_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_July_2009 19]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/20_July_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_July_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_July_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_July_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_July_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_July_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_July_2009 26]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/27_July_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_July_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_July_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_July_2009 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_July_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_August_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_August_2009 2]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/3_August_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_August_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_August_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_August_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_August_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_August_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_August_2009 9]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/10_August_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_August_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_August_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_August_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_August_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_August_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_August_2009 16]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/17_August_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_August_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_August_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_August_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_August_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_August_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_August_2009 23]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/24_August_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_August_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_August_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_August_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_August_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_August_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_August_2009 30]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_September_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_September_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_September_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_September_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_September_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_September_2009 6]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/7_September_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_September_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_September_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_September_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_September_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_September_2009 13]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/14_September_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_September_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_September_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_September_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_September_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_September_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_September_2009 20]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/21_September_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_September_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_September_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_September_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_September_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_September_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_September_2009 27]
[http://2009.igem.org/Team:Warsaw/Calendar-Main/28_September_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_September_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_October_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_October_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_October_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_October_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_October_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/28_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]