Team:UNICAMP-Brazil/Notebooks/August 19

(Difference between revisions)

Latest revision as of 01:10, 22 October 2009

In the LB-AMP plates we managed to get isolated colonies, but the LB-KAN plates were the same as the first we had. We think that the antibiotic is not working on these LB-KAN plates, since the supposed antibiotic for the terminator vector is ampicilin.

We selected colonies from the LB-AMP plates and inoculated in LB liquid media for the miniprep tomorrow.

Taís

Today, we performed two enzymatic digestions of two RBS, recovered in order to prove the transformations. We got it!!!

Luige

@@ Line 8: / Line 8: @@
 ====Recovering the biobricks====
-In the LB-AMP plates we manage to get isolated colonies, but the LB-KAN plates were the same as the first we had. We think that the antibiotic is not working in these LB-KAN plates, since the supposed antibiotic for the terminator vector is ampicilin.
+*<p style=”text-align:justify;”>In the LB-AMP plates we managed to get isolated colonies, but the LB-KAN plates were the same as the first we had. We think that the antibiotic is not working on these LB-KAN plates, since the supposed antibiotic for the terminator vector is ampicilin.</p>
-We selected colonies from the LB-AMP plates and inoculated in LB liquid media for the miniprep tomorrow.
-We also inoculated colonies from the RBS plate for the miniprep.
+*<p style=”text-align:justify;”>We selected colonies from the LB-AMP plates and inoculated in LB liquid media for the miniprep tomorrow.</p>
+*<p style=”text-align:justify;”>We also inoculated colonies from the RBS plate for the miniprep.</p>
 ''Taís''
@@ Line 17: / Line 18: @@
 ==''' ColiGuard '''==
-====Enzime restriction====
+====Enzyme restriction====
-Today, we performed two enzyme restrictions of two RBSs recovered in order to prove the transformations. We got it!!!
+*<p style=”text-align:justify;”>Today, we performed two enzymatic digestions of two RBS, recovered in order to prove the transformations. We got it!!!
 ''Luige''