Team:EPF-Lausanne/Notebook

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{{EPF-Lausanne09}}
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=Notebook=
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===06.07.09===
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<font size="12" color="#007CBC">Notebook</font>
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[[Team:EPF-Lausanne/Notebook/Wet Lab|'''Wet Lab''']]
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<p align="center"><big>'''{{CURRENTDAY}}.{{CURRENTMONTH}}.{{CURRENTYEAR}} | {{CURRENTTIME}}'''</big></p></FONT>
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[[Team:EPF-Lausanne/Notebook/Cloning Strategy|'''Cloning Strategy''']]
 
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;People in the lab: Tu, Heidi, Rafael, Basile, Nath
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==Calendar==
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===07.07.09===
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<center><table valign=top>
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<td valign=top>{{ #calendar: title=EPF-Lausanne |year=2009 | month=06 }}
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=07 }}
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=08 }}
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=09 }}
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=10 }}
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;Remark for the notebook:
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First, it's great you already started to use the wiki and customised the menu!<br> Then I think we should add the name of the peoples who worked on each part of a process (or at least present the same day). It would allow easy team transitions.<br>
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<iframe src="http://www.google.com/calendar/embed?title=EPFL Planning &amp;height=600&amp;wkst=1&amp;bgcolor=%23FFFFFF&amp;src=igem.epfl.09%40gmail.com&amp;color=%23A32929&amp;ctz=Europe%2FRome" style=" border-width:0 " width="800" height="600" frameborder="0" scrolling="no"></iframe>
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For the wiki in general, as you did in this page, it is much better not to use html tags.<br>
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We will have a meeting for the modeling on tuesday, I will come to the lab before.<br>
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;Cloning strategy:
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To design plasmids : software Vector NTI
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;Wet lab:
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We have to grow the 3 strains generously sent by [mailto:j.beatty@ubc.ca Tom Beatty]
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The three strains are :
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:*''R.Palustris'' CEA001 (wild type) ; should be grown on LB medium only
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:*''R.Palustris'' BPHP1+ ; should be grown on LB with gentamycin (100 micrograms/ml)
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:*''E.Coli'' DH10B (pBPH/hmu0) ; should be grown on LB with gentamycin (20 micorgrams/ml)
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The transformed LOVTAP and TrpR worked well (N.B. the plasmid of TrpR is pUC19 so the antibiotic resistance is Amp -> see below)
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[[Image: RTEmagicC_puc19_2.gif.gif|500px|thumb|center|pUC19 plasmid]]
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We did the glycerol stock, located in the -80 fridge, first floor of the iGEM compartement.
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Then, a miniprep was done with both cultures.
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A LOVTAP plasmid aliquot was done, a TrpR plasmid aliquot was done, located in the -20 fridge, 2nd floor.
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'''People in the lab'''
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:Tu, Rafael, Nath, Heidi, Basile
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===08.07.09===
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{| style="color:#1b2c8a;background-color:#0c9;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:EPF-Lausanne|Home]]
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!align="center"|[[Team:EPF-Lausanne/Team|The Team]]
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!align="center"|[[Team:EPF-Lausanne/Project|The Project]]
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!align="center"|[[Team:EPF-Lausanne/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:EPF-Lausanne/Modeling|Modeling]]
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!align="center"|[[Team:EPF-Lausanne/Notebook|Notebook]]
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!align="center"|[[Team:EPF-Lausanne/Lectures|Lectures]]
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!align="center"|[[Team:EPF-Lausanne/Team Management|Team Management]]
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!align="center"|[[Team:EPF-Lausanne/References|References]]
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Latest revision as of 18:24, 20 October 2009



Contents








Notebook


14.12.2024 | 23:50


Calendar

           
June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/29_June_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/30_June_2009&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/2_July_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/5_July_2009&action=edit 5]
[http://2009.igem.org/EPF-Lausanne/6_July_2009 6] [http://2009.igem.org/EPF-Lausanne/7_July_2009 7] [http://2009.igem.org/EPF-Lausanne/8_July_2009 8] [http://2009.igem.org/EPF-Lausanne/9_July_2009 9] [http://2009.igem.org/EPF-Lausanne/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/12_July_2009&action=edit 12]
[http://2009.igem.org/EPF-Lausanne/13_July_2009 13] [http://2009.igem.org/EPF-Lausanne/14_July_2009 14] [http://2009.igem.org/EPF-Lausanne/15_July_2009 15] [http://2009.igem.org/EPF-Lausanne/16_July_2009 16] [http://2009.igem.org/EPF-Lausanne/17_July_2009 17] [http://2009.igem.org/EPF-Lausanne/18_July_2009 18] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/19_July_2009&action=edit 19]
[http://2009.igem.org/EPF-Lausanne/20_July_2009 20] [http://2009.igem.org/EPF-Lausanne/21_July_2009 21] [http://2009.igem.org/EPF-Lausanne/22_July_2009 22] [http://2009.igem.org/EPF-Lausanne/23_July_2009 23] [http://2009.igem.org/EPF-Lausanne/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/26_July_2009&action=edit 26]
[http://2009.igem.org/EPF-Lausanne/27_July_2009 27] [http://2009.igem.org/EPF-Lausanne/28_July_2009 28] [http://2009.igem.org/EPF-Lausanne/29_July_2009 29] [http://2009.igem.org/EPF-Lausanne/30_July_2009 30] [http://2009.igem.org/EPF-Lausanne/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/EPF-Lausanne/1_August_2009 1] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/2_August_2009&action=edit 2]
[http://2009.igem.org/EPF-Lausanne/3_August_2009 3] [http://2009.igem.org/EPF-Lausanne/4_August_2009 4] [http://2009.igem.org/EPF-Lausanne/5_August_2009 5] [http://2009.igem.org/EPF-Lausanne/6_August_2009 6] [http://2009.igem.org/EPF-Lausanne/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/9_August_2009&action=edit 9]
[http://2009.igem.org/EPF-Lausanne/10_August_2009 10] [http://2009.igem.org/EPF-Lausanne/11_August_2009 11] [http://2009.igem.org/EPF-Lausanne/12_August_2009 12] [http://2009.igem.org/EPF-Lausanne/13_August_2009 13] [http://2009.igem.org/EPF-Lausanne/14_August_2009 14] [http://2009.igem.org/EPF-Lausanne/15_August_2009 15] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/16_August_2009&action=edit 16]
[http://2009.igem.org/EPF-Lausanne/17_August_2009 17] [http://2009.igem.org/EPF-Lausanne/18_August_2009 18] [http://2009.igem.org/EPF-Lausanne/19_August_2009 19] [http://2009.igem.org/EPF-Lausanne/20_August_2009 20] [http://2009.igem.org/EPF-Lausanne/21_August_2009 21] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/23_August_2009&action=edit 23]
[http://2009.igem.org/EPF-Lausanne/24_August_2009 24] [http://2009.igem.org/EPF-Lausanne/25_August_2009 25] [http://2009.igem.org/EPF-Lausanne/26_August_2009 26] [http://2009.igem.org/EPF-Lausanne/27_August_2009 27] [http://2009.igem.org/EPF-Lausanne/28_August_2009 28] [http://2009.igem.org/EPF-Lausanne/29_August_2009 29] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/30_August_2009&action=edit 30]
[http://2009.igem.org/EPF-Lausanne/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/EPF-Lausanne/1_September_2009 1] [http://2009.igem.org/EPF-Lausanne/2_September_2009 2] [http://2009.igem.org/EPF-Lausanne/3_September_2009 3] [http://2009.igem.org/EPF-Lausanne/4_September_2009 4] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/6_September_2009&action=edit 6]
[http://2009.igem.org/EPF-Lausanne/7_September_2009 7] [http://2009.igem.org/EPF-Lausanne/8_September_2009 8] [http://2009.igem.org/EPF-Lausanne/9_September_2009 9] [http://2009.igem.org/EPF-Lausanne/10_September_2009 10] [http://2009.igem.org/EPF-Lausanne/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/13_September_2009&action=edit 13]
[http://2009.igem.org/EPF-Lausanne/14_September_2009 14] [http://2009.igem.org/EPF-Lausanne/15_September_2009 15] [http://2009.igem.org/EPF-Lausanne/16_September_2009 16] [http://2009.igem.org/EPF-Lausanne/17_September_2009 17] [http://2009.igem.org/EPF-Lausanne/18_September_2009 18] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/19_September_2009&action=edit 19] [http://2009.igem.org/EPF-Lausanne/20_September_2009 20]
[http://2009.igem.org/EPF-Lausanne/21_September_2009 21] [http://2009.igem.org/EPF-Lausanne/22_September_2009 22] [http://2009.igem.org/EPF-Lausanne/23_September_2009 23] [http://2009.igem.org/EPF-Lausanne/24_September_2009 24] [http://2009.igem.org/EPF-Lausanne/25_September_2009 25] [http://2009.igem.org/EPF-Lausanne/26_September_2009 26] [http://2009.igem.org/EPF-Lausanne/27_September_2009 27]
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/28_September_2009&action=edit 28] [http://2009.igem.org/EPF-Lausanne/29_September_2009 29] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/30_September_2009&action=edit 30]
October
MTWTFSS
      [http://2009.igem.org/EPF-Lausanne/1_October_2009 1] [http://2009.igem.org/EPF-Lausanne/2_October_2009 2] [http://2009.igem.org/EPF-Lausanne/3_October_2009 3] [http://2009.igem.org/EPF-Lausanne/4_October_2009 4]
[http://2009.igem.org/EPF-Lausanne/5_October_2009 5] [http://2009.igem.org/EPF-Lausanne/6_October_2009 6] [http://2009.igem.org/EPF-Lausanne/7_October_2009 7] [http://2009.igem.org/EPF-Lausanne/8_October_2009 8] [http://2009.igem.org/EPF-Lausanne/9_October_2009 9] [http://2009.igem.org/EPF-Lausanne/10_October_2009 10] [http://2009.igem.org/EPF-Lausanne/11_October_2009 11]
[http://2009.igem.org/EPF-Lausanne/12_October_2009 12] [http://2009.igem.org/EPF-Lausanne/13_October_2009 13] [http://2009.igem.org/EPF-Lausanne/14_October_2009 14] [http://2009.igem.org/EPF-Lausanne/15_October_2009 15] [http://2009.igem.org/EPF-Lausanne/16_October_2009 16] [http://2009.igem.org/EPF-Lausanne/17_October_2009 17] [http://2009.igem.org/EPF-Lausanne/18_October_2009 18]
[http://2009.igem.org/EPF-Lausanne/19_October_2009 19] [http://2009.igem.org/EPF-Lausanne/20_October_2009 20] [http://2009.igem.org/EPF-Lausanne/21_October_2009 21] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=EPF-Lausanne/31_October_2009&action=edit 31]
Retrieved from "http://2009.igem.org/Team:EPF-Lausanne/Notebook"