Team:UNC Chapel Hill/Project Needs

From 2009.igem.org

(Difference between revisions)
(→Basic Lab Supplies)
(→Basic Lab Supplies)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
==Basic Lab Supplies==
==Basic Lab Supplies==
-
# Petri Dishes
+
# Blank Petri Dishes
-
# Gloves
+
# Electric cuvettes
-
# Screw Top Microcentrifuge Tubes
+
# MiniPrep Kit
-
# Cell Culture Tubes
+
# Disposable Gloves (S, M, L)
-
# Eppendorf Tubes
+
# Screw Top Microcentrifuge Tubes (1.5 mL)
-
# 9 mL glass Pasteur pipets
+
# Cell Culture Tubes (10 mL)
 +
# Eppendorf Tubes (1.5 mL)
 +
# Glass Pasteur pipets (9 mL)
# Parafilm
# Parafilm
# Micropipets (P2, P20, P200, P1000)
# Micropipets (P2, P20, P200, P1000)
-
# Micropipet Tips
+
# Micropipet Tips (for all sizes listed above)
-
# Restriction Enzymes-if from Fermentas, should be FastDigest versions.  If NEB, we need EcoRI-HF.
+
# Restriction Enzymes-if from Fermentas, should be FastDigest versions.  If NEB, we need EcoRI-HF:
## EcoRI
## EcoRI
## Xbal
## Xbal
Line 16: Line 18:
## MunI (MfeI)
## MunI (MfeI)
## Mph1103I (NsiI)
## Mph1103I (NsiI)
-
# T4 DNA Ligase
+
## T4 DNA Ligase
# Agarose Powder
# Agarose Powder
-
# Luria Broth
+
# Luria Broth and/or Super Optimal Broth
-
# Buffer
+
# Buffer for Gel Electrophoresis
# Antibiotics:
# Antibiotics:
## Carbenicillin
## Carbenicillin
Line 26: Line 28:
# PCR Supermix
# PCR Supermix
# Competent Cells (DH10B, Top10, or DH5a.  DB3.1)
# Competent Cells (DH10B, Top10, or DH5a.  DB3.1)
 +
# Power Generators for GE
 +
# Gel Rigs
 +
# Gel Combs
==Equipment==
==Equipment==
Line 32: Line 37:
==Other==
==Other==
-
#Plane tickets to Boston for Jamboree weekend
+
#Plane tickets to Boston for Jamboree weekend (October 31 to November 2nd, 2009)
-
#Hotel stay in Boston
+
#Hotel stay in Boston for Jamboree
 +
#Attendance fee for Jamboree ($175 per student; $375 per advisor)
#Custom T-shirts
#Custom T-shirts

Latest revision as of 16:11, 11 July 2009

Basic Lab Supplies

  1. Blank Petri Dishes
  2. Electric cuvettes
  3. MiniPrep Kit
  4. Disposable Gloves (S, M, L)
  5. Screw Top Microcentrifuge Tubes (1.5 mL)
  6. Cell Culture Tubes (10 mL)
  7. Eppendorf Tubes (1.5 mL)
  8. Glass Pasteur pipets (9 mL)
  9. Parafilm
  10. Micropipets (P2, P20, P200, P1000)
  11. Micropipet Tips (for all sizes listed above)
  12. Restriction Enzymes-if from Fermentas, should be FastDigest versions. If NEB, we need EcoRI-HF:
    1. EcoRI
    2. Xbal
    3. SpeI
    4. PstI
    5. MunI (MfeI)
    6. Mph1103I (NsiI)
    7. T4 DNA Ligase
  13. Agarose Powder
  14. Luria Broth and/or Super Optimal Broth
  15. Buffer for Gel Electrophoresis
  16. Antibiotics:
    1. Carbenicillin
    2. Kanamycin
  17. Gel Extraction Kit
  18. PCR Supermix
  19. Competent Cells (DH10B, Top10, or DH5a. DB3.1)
  20. Power Generators for GE
  21. Gel Rigs
  22. Gel Combs

Equipment

  1. Thermal Cycler
  2. Fluorometer

Other

  1. Plane tickets to Boston for Jamboree weekend (October 31 to November 2nd, 2009)
  2. Hotel stay in Boston for Jamboree
  3. Attendance fee for Jamboree ($175 per student; $375 per advisor)
  4. Custom T-shirts
Retrieved from "http://2009.igem.org/Team:UNC_Chapel_Hill/Project_Needs"