Team:UNIPV-Pavia/Freezer
From 2009.igem.org
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<table width="100%"> | <table width="100%"> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
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</html> | </html> | ||
__NOTOC__ | __NOTOC__ | ||
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<td align="center" width="165px" valign="center"><font face="papyrus" color="royalblue"><b>Description</td> | <td align="center" width="165px" valign="center"><font face="papyrus" color="royalblue"><b>Description</td> | ||
<td align="center" width="73px" valign="center"><font face="papyrus" color="royalblue"><b>Creation Date</td> | <td align="center" width="73px" valign="center"><font face="papyrus" color="royalblue"><b>Creation Date</td> | ||
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<td align="center" width="60px" valign="center"><font face="papyrus" color="royalblue"><b>Strain</td> | <td align="center" width="60px" valign="center"><font face="papyrus" color="royalblue"><b>Strain</td> | ||
<td align="center" width="97px" valign="center"><font face="papyrus" color="royalblue"><b>Vector</td> | <td align="center" width="97px" valign="center"><font face="papyrus" color="royalblue"><b>Vector</td> | ||
<td align="center" valign="center"><font face="papyrus" color="royalblue"><b>Quality Control</td> | <td align="center" valign="center"><font face="papyrus" color="royalblue"><b>Quality Control</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">J23101(J101)</td> | + | <tr class="in_freezer"><td class="prova">J23100(J100)<br>replaced by "J23100 Jun4"</td> |
+ | <td class="prova">constitutive promoter</td> | ||
+ | <td class="prova">May 25</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">J61002(Amp)</td> | ||
+ | <td class="prova">sequence ok (MIT)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">J23101(J101)<br>replaced by "J23101 Jun4"</td> | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 53: | Line 54: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 61: | Line 61: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 69: | Line 68: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 77: | Line 75: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 85: | Line 82: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 93: | Line 89: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 101: | Line 96: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 109: | Line 103: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 117: | Line 110: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 125: | Line 117: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 133: | Line 124: | ||
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
<td class="prova">sequence ok (MIT)</td> | <td class="prova">sequence ok (MIT)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">J23118(J118)</td> | + | <tr class="in_freezer"><td class="prova">J23118(J118)<br>replaced by "J23118 Jun4"</td> |
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
Line 149: | Line 138: | ||
<td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td> | <td class="prova">GFP protein generator<br>(RBS-GFP-TT)</td> | ||
<td class="prova">May 25</td> | <td class="prova">May 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 157: | Line 145: | ||
<td class="prova">colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)</td> | <td class="prova">colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 165: | Line 152: | ||
<td class="prova">colE7 lysis gene</td> | <td class="prova">colE7 lysis gene</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 173: | Line 159: | ||
<td class="prova">Phage Lysis Cassette</td> | <td class="prova">Phage Lysis Cassette</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 181: | Line 166: | ||
<td class="prova">Double terminator</td> | <td class="prova">Double terminator</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
Line 189: | Line 173: | ||
<td class="prova">RBS-lacZ</td> | <td class="prova">RBS-lacZ</td> | ||
<td class="prova">May 28</td> | <td class="prova">May 28</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 197: | Line 180: | ||
<td class="prova">RBS 0.6</td> | <td class="prova">RBS 0.6</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 205: | Line 187: | ||
<td class="prova">RBS 0.07</td> | <td class="prova">RBS 0.07</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 213: | Line 194: | ||
<td class="prova">RBS 0.3</td> | <td class="prova">RBS 0.3</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 221: | Line 201: | ||
<td class="prova">RBS 0.01</td> | <td class="prova">RBS 0.01</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 229: | Line 208: | ||
<td class="prova">RBS 1</td> | <td class="prova">RBS 1</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 237: | Line 215: | ||
<td class="prova">RBS-tetR-TT</td> | <td class="prova">RBS-tetR-TT</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 245: | Line 222: | ||
<td class="prova">Ptet</td> | <td class="prova">Ptet</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 253: | Line 229: | ||
<td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td> | <td class="prova">Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A3</td> | <td class="prova">pSB1A3</td> | ||
<td class="prova">sequence BAD (MIT) - sequence ok (BMR)</td> | <td class="prova">sequence BAD (MIT) - sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">J23100( | + | <tr class="in_freezer"><td class="prova">J23100(J100 Jun4)</td> |
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
<td class="prova">sequence ok (MIT,BMR)</td> | <td class="prova">sequence ok (MIT,BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">J23101( | + | <tr class="in_freezer"><td class="prova">J23101(J101 Jun4)</td> |
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
<td class="prova">sequence ok (MIT)</td> | <td class="prova">sequence ok (MIT)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">J23118( | + | <tr class="in_freezer"><td class="prova">J23118(J118 Jun4)</td> |
<td class="prova">constitutive promoter</td> | <td class="prova">constitutive promoter</td> | ||
<td class="prova">Jun 4</td> | <td class="prova">Jun 4</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">J61002(Amp)</td> | <td class="prova">J61002(Amp)</td> | ||
<td class="prova">sequence ok (MIT)</td> | <td class="prova">sequence ok (MIT)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A1</td> | + | <tr class="in_freezer"><td class="prova">A1 (BBa_K173000)</td> |
<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | <td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 11</td> | <td class="prova">Jun 11</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A2</td> | + | <tr class="in_freezer"><td class="prova">A2 (BBa_K173001)</td> |
<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | <td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 11</td> | <td class="prova">Jun 11</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A3</td> | + | <tr class="in_freezer"><td class="prova">A3-1 (BBa_K173004)</td> |
<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td> | <td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td> | ||
<td class="prova">Jun 12</td> | <td class="prova">Jun 12</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
- | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion)</a> sequence ok -long part- (BMR)</td> | + | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion PstI)</a> sequence ok -long part- (BMR)</td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A4</td> | + | <tr class="in_freezer"><td class="prova">A4 (BBa_K173005)</td> |
<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td> | <td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td> | ||
<td class="prova">Jun 11</td> | <td class="prova">Jun 11</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td> | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A5</td> | + | <tr class="in_freezer"><td class="prova">A5 (BBa_K173006)</td> |
<td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td> | <td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td> | ||
<td class="prova">Jun 19</td> | <td class="prova">Jun 19</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
<td class="prova">sequence ok -long part- (BMR)</td> | <td class="prova">sequence ok -long part- (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A6</td> | + | <tr class="in_freezer"><td class="prova">A6 (BBa_K173007)</td> |
<td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td> | <td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td> | ||
<td class="prova">Jun 19</td> | <td class="prova">Jun 19</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 333: | Line 299: | ||
<td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td> | <td class="prova">J23118-B0034-C0012-B0015<br>(Pcon-RBS-lacI-TT)</td> | ||
<td class="prova">Jun 23</td> | <td class="prova">Jun 23</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
<td class="prova">sequence ok (BMR)</td> | <td class="prova">sequence ok (BMR)</td> | ||
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A7</td> | + | <tr class="in_freezer"><td class="prova">A7 (BBa_K173002)</td> |
<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | <td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 25</td> | <td class="prova">Jun 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 349: | Line 313: | ||
<td class="prova">Plac</td> | <td class="prova">Plac</td> | ||
<td class="prova">Jun 25</td> | <td class="prova">Jun 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
Line 357: | Line 320: | ||
<td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td> | <td class="prova">holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)</td> | ||
<td class="prova">Jun 25</td> | <td class="prova">Jun 25</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">sequence ok -long part- (MIT)</td> | + | <td class="prova">sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010).</td> |
</tr> | </tr> | ||
<tr class="in_freezer"><td class="prova">A8</td> | <tr class="in_freezer"><td class="prova">A8</td> | ||
<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 29</td> | <td class="prova">Jun 29</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion)</a> but works</td> | + | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion E-P)</a> but works</td> |
</tr> | </tr> | ||
<tr class="in_freezer"><td class="prova">A9</td> | <tr class="in_freezer"><td class="prova">A9</td> | ||
<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jun 29</td> | <td class="prova">Jun 29</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion)</a> but works</td> | + | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9.jpg">(digestion E-P)</a> but works</td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A10</td> | + | <tr class="in_freezer"><td class="prova">A10<br>thrown away</td> |
<td class="prova">K117000-B0015<br>(celB-TT)</td> | <td class="prova">K117000-B0015<br>(celB-TT)</td> | ||
<td class="prova">Jun 30</td> | <td class="prova">Jun 30</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1AK3</td> | <td class="prova">pSB1AK3</td> | ||
- | <td class="prova">sequence BAD | + | <td class="prova">sequence BAD (BMR)</td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A8pg</td> | + | <tr class="in_freezer"><td class="prova">A8pg (BBa_K173008)</td> |
<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jul 3</td> | <td class="prova">Jul 3</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion)</a></td> | + | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A9pg</td> | + | <tr class="in_freezer"><td class="prova">A9pg (BBa_K173009)</td> |
<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | <td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
<td class="prova">Jul 3</td> | <td class="prova">Jul 3</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
- | <td class="prova"> | + | <td class="prova">pSB1A2</td> |
- | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion)</a></td> | + | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-1</td> | + | <tr class="in_freezer"><td class="prova">A11-1<br>thrown away and replaced</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">length ok <a href="https://2009.igem.org/Image: | + | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a><br>sequence MUTATED</td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-3</td> | + | <tr class="in_freezer"><td class="prova">A11-3<br>thrown away and replaced</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">length ok <a href="https://2009.igem.org/Image: | + | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">A11-6</td> | + | <tr class="in_freezer"><td class="prova">A11-6<br>thrown away and replaced</td> |
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">length ok <a href="https://2009.igem.org/Image: | + | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A11.jpg">(PCR)</a></td> |
</tr> | </tr> | ||
- | <tr class="in_freezer"><td class="prova">F2620</td> | + | <tr class="in_freezer"><td class="prova">F2620<br>thrown away</td> |
<td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | <td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | ||
<td class="prova">Jul 10</td> | <td class="prova">Jul 10</td> | ||
- | |||
<td class="prova">TOP10</td> | <td class="prova">TOP10</td> | ||
<td class="prova">pSB1A2</td> | <td class="prova">pSB1A2</td> | ||
- | <td class="prova">sequence BAD (MIT)</td> | + | <td class="prova">sequence BAD (MIT, BMR)</td> |
</tr> | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A12-2 (BBa_K173011)</td> | ||
+ | <td class="prova">J23118(Jun 4)-A4<br></td> | ||
+ | <td class="prova">Jul 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A12-3<br>it is a backup of A12-2</td> | ||
+ | <td class="prova">J23118(Jun 4)-A4<br></td> | ||
+ | <td class="prova">Jul 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A12.jpg">(PCR)</a><br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A14-4<br>thrown away</td> | ||
+ | <td class="prova">A11-E0240<br></td> | ||
+ | <td class="prova">Jul 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A14pg <br>thrown away</td> | ||
+ | <td class="prova">A11-E0240<br></td> | ||
+ | <td class="prova">Jul 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K116001 (PnhaA)</td> | ||
+ | <td class="prova">PnhaA-RBS-GFP-TT<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence WRONG! it is actually K116002!!!</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td> | ||
+ | <td class="prova">PnhaA-RBS-GFP-TT<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence WRONG! it is actually J33204!!!</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K112405 (Pcad)</td> | ||
+ | <td class="prova">cadAB promoter<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">K112951(Amp+Cm)</td> | ||
+ | <td class="prova">sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">P0412</td> | ||
+ | <td class="prova">RBS-lacI-TT<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">DH5alpha</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">I746902</td> | ||
+ | <td class="prova">GFPmut3+his tag generator under Pbad<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">I746903</td> | ||
+ | <td class="prova">GFPmut3+his tag generator under PT7<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K101017<br>to throw away!</td> | ||
+ | <td class="prova">MioC promoter<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">NEB 10-beta</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence BAD (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">F2620MIT1</td> | ||
+ | <td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">D1210(lacIq)</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">F2620MIT2</td> | ||
+ | <td class="prova">Ptet-RBS-luxR-TT-Plux<br></td> | ||
+ | <td class="prova">Jul 29</td> | ||
+ | <td class="prova">D1210(lacIq)</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">MRGENE1 (BBa_K173016)</td> | ||
+ | <td class="prova">pdc</td> | ||
+ | <td class="prova">Jul 17</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pMK-RQ<br>(Kan)</td> | ||
+ | <td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_Digestion_mrGene_B0030.jpg">(digestion X-P)</a><br>sequence ok (Mr Gene)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">MRGENE1-2</td> | ||
+ | <td class="prova">pdc</td> | ||
+ | <td class="prova">Jul 17</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pMK-RQ<br>(Kan)</td> | ||
+ | <td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">MRGENE1-3</td> | ||
+ | <td class="prova">pdc</td> | ||
+ | <td class="prova">Jul 17</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pMK-RQ<br>(Kan)</td> | ||
+ | <td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">MRGENE2 (BBa_K173017)</td> | ||
+ | <td class="prova">adhB</td> | ||
+ | <td class="prova">Jul 17</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pMA<br>(Amp)</td> | ||
+ | <td class="prova">antibiotic ok<br>length ok <a href="https://2009.igem.org/Image:Pv_Digestion_mrGene_B0030.jpg">(digestion X-P)</a><br>sequence ok (Mr Gene)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">MRGENE2-2</td> | ||
+ | <td class="prova">adhB</td> | ||
+ | <td class="prova">Jul 17</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pMA<br>(Amp)</td> | ||
+ | <td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">MRGENE2-3</td> | ||
+ | <td class="prova">adhB</td> | ||
+ | <td class="prova">Jul 17</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pMA<br>(Amp)</td> | ||
+ | <td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B1-13 (BBa_K173013)</td> | ||
+ | <td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td> | ||
+ | <td class="prova">Jul 28</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion E-P</a><br>, <a href="https://2009.igem.org/Image:Pv_digestion_B1_S-P.jpg">digestion S-P</a> and <a href="https://2009.igem.org/Image:Pv_B1E-S_B2E-S_B0015E-X_cut.jpg">digestion E-S</a>)<br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B2-5 (BBa_K173014)</td> | ||
+ | <td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td> | ||
+ | <td class="prova">Jul 23</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_B2pcr_A14pgPstI.jpg">PCR</a><br> and <a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion S-P</a>)<br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B3-5 (BBa_K173018)</td> | ||
+ | <td class="prova">B1-B0015<br>(RBS-pdc-TT)</td> | ||
+ | <td class="prova">Jul 31</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok+extra band (<a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_B3_digestion_X-P.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B4-2 (BBa_K173019)</td> | ||
+ | <td class="prova">B2-B0015<br>(RBS-adhB-TT)</td> | ||
+ | <td class="prova">Jul 31</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">(digestion XhoI-P)</a><br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B5-3</td> | ||
+ | <td class="prova">B1-B4<br>(RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Aug 12</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B6-3 (BBa_K173020)</td> | ||
+ | <td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td> | ||
+ | <td class="prova">Aug 12</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A11-2<br>thrown away</td> | ||
+ | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
+ | <td class="prova">Aug 13</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence MUTATED (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A11-3 (BBa_K173010)</td> | ||
+ | <td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td> | ||
+ | <td class="prova">Aug 13</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A15-1<br>it is a backup copy of A15-3</td> | ||
+ | <td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
+ | <td class="prova">Aug 14</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A15-2<br>thrown away</td> | ||
+ | <td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
+ | <td class="prova">Aug 14</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence WRONG (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A15-3 (BBa_K173015)</td> | ||
+ | <td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
+ | <td class="prova">Aug 14</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B8-5<br>thrown away</td> | ||
+ | <td class="prova">A4-B6<br>(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)</td> | ||
+ | <td class="prova">Aug 21</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ??? (digestion E-P) sequence WRONG (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A14-3<br>to throw away!</td> | ||
+ | <td class="prova">A11(-2 Aug13)-E0240<br>(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)</td> | ||
+ | <td class="prova">Aug 21</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence of A11-2(Aug13) was MUTATED (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A16-4 (BBa_K173026)</td> | ||
+ | <td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
+ | <td class="prova">Aug 21</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A17-4<br>to throw away!</td> | ||
+ | <td class="prova">R0011-E0240<br>(Plac-RBS-GFP-TT)</td> | ||
+ | <td class="prova">Aug 28</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence WRONG! ligation failed.</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A18<br>thrown away</td> | ||
+ | <td class="prova">A15(-2)-pSB3K3<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td> | ||
+ | <td class="prova">Aug 28</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB3K3</td> | ||
+ | <td class="prova">not screened, its parent plasmid (A15-2) was wrong</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">K116002 (PnhaA+GFP)</td> | ||
+ | <td class="prova">PnhaA-RBS-GFP-TT<br></td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B5new2-3 (BBa_K173003)</td> | ||
+ | <td class="prova">B1-B4 (RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Sep 1</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B9-2 (BBa_K173021)</td> | ||
+ | <td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B10-2 (BBa_K173022)</td> | ||
+ | <td class="prova">F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT)</td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok + 2 extra bands (digestion E-P), sequence ok -long part- (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B9new-1 to 5</td> | ||
+ | <td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Oct 1</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1AK3</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A19</td> | ||
+ | <td class="prova">F2620</td> | ||
+ | <td class="prova">Oct 1</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB4C5</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A21</td> | ||
+ | <td class="prova">A19-B5new2(-3)</td> | ||
+ | <td class="prova">Oct 8</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB4C5</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A14S-3 (BBa_K173012)</td> | ||
+ | <td class="prova">J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240)</td> | ||
+ | <td class="prova">?</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A17new (BBa_K173025)</td> | ||
+ | <td class="prova">Plac-RBS-GFP-TT (R0011-E0240)</td> | ||
+ | <td class="prova">Sep 4</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (PCR), sequence ok (BMR)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">A20</td> | ||
+ | <td class="prova">F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td> | ||
+ | <td class="prova">Oct 8</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">I52002 (4C5)</td> | ||
+ | <td class="prova">plasmid</td> | ||
+ | <td class="prova">?</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB4C5</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">I714891 (3K3)</td> | ||
+ | <td class="prova">plasmid</td> | ||
+ | <td class="prova">Aug 21</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB3K3</td> | ||
+ | <td class="prova">length ok (digestion E-P)</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B14 (BBa_K173023)</td> | ||
+ | <td class="prova">F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT)</td> | ||
+ | <td class="prova">Oct 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">to screen</td> | ||
+ | </tr> | ||
+ | <tr class="in_freezer"><td class="prova">B15 (BBa_K173024)</td> | ||
+ | <td class="prova">F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT)</td> | ||
+ | <td class="prova">Oct 16</td> | ||
+ | <td class="prova">TOP10</td> | ||
+ | <td class="prova">pSB1A2</td> | ||
+ | <td class="prova">to screen</td> | ||
+ | </tr> | ||
+ | |||
</table> | </table> | ||
</td> | </td> |
Latest revision as of 12:32, 21 October 2009
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