Team:UNC Chapel Hill/Project Needs
From 2009.igem.org
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## MunI (MfeI) | ## MunI (MfeI) | ||
## Mph1103I (NsiI) | ## Mph1103I (NsiI) | ||
- | # T4 DNA Ligase | + | ## T4 DNA Ligase |
# Agarose Powder | # Agarose Powder | ||
# Luria Broth and/or Super Optimal Broth | # Luria Broth and/or Super Optimal Broth | ||
Line 28: | Line 28: | ||
# PCR Supermix | # PCR Supermix | ||
# Competent Cells (DH10B, Top10, or DH5a. DB3.1) | # Competent Cells (DH10B, Top10, or DH5a. DB3.1) | ||
- | + | # Power Generators for GE | |
+ | # Gel Rigs | ||
+ | # Gel Combs | ||
==Equipment== | ==Equipment== |
Latest revision as of 16:11, 11 July 2009
Basic Lab Supplies
- Blank Petri Dishes
- Electric cuvettes
- MiniPrep Kit
- Disposable Gloves (S, M, L)
- Screw Top Microcentrifuge Tubes (1.5 mL)
- Cell Culture Tubes (10 mL)
- Eppendorf Tubes (1.5 mL)
- Glass Pasteur pipets (9 mL)
- Parafilm
- Micropipets (P2, P20, P200, P1000)
- Micropipet Tips (for all sizes listed above)
- Restriction Enzymes-if from Fermentas, should be FastDigest versions. If NEB, we need EcoRI-HF:
- EcoRI
- Xbal
- SpeI
- PstI
- MunI (MfeI)
- Mph1103I (NsiI)
- T4 DNA Ligase
- Agarose Powder
- Luria Broth and/or Super Optimal Broth
- Buffer for Gel Electrophoresis
- Antibiotics:
- Carbenicillin
- Kanamycin
- Gel Extraction Kit
- PCR Supermix
- Competent Cells (DH10B, Top10, or DH5a. DB3.1)
- Power Generators for GE
- Gel Rigs
- Gel Combs
Equipment
- Thermal Cycler
- Fluorometer
Other
- Plane tickets to Boston for Jamboree weekend (October 31 to November 2nd, 2009)
- Hotel stay in Boston for Jamboree
- Attendance fee for Jamboree ($175 per student; $375 per advisor)
- Custom T-shirts