From 2009.igem.org
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| - | {{Template:TUDelftiGEM2009}}
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| - | ''Page is under construction''
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| - | <br>
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| - | ='''Project Overview'''=
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| - | __TOC__
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| - | =='''Our abstract'''==
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| - | <br>
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| - | Finally TUDelft team has a project!!!
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| - | ===Self-destructive plasmid===
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| - | [[Image:TUDelft_logo.png|50px|right|frame]]
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| - | '''Research question:'''
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| - | Can we achieve cell-to-cell communication:
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| - | #Using delay device
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| - | #Using self-destructive plasmid
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| - | #Between two F+ cells
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| - | '''Goals:'''
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| - | <br>
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| - | *Build a delay device that can change the timing of transcribing the target protein
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| - | *Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
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| - | *Construct a modified F plasmid that will allow F+/F+ conjugation
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| - | <br>
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| - | '''Why?'''
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| - | <br>
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| - | *To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
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| - | *To generate a pulse in the expression of a certain gene
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| - | *To control the amplitude of the pulse created by the plasmid
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| - | *Because it is cool!!!
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| - | <br><br>
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| - | '''How?'''
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| - | <br>
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| - | Not known yet :D
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| - | <br><br>
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| - | '''Applications:'''
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| - | <br>
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| - | *Cell synchronization
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| - | *Selectable marker recycling
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| - | *Metabolic control analysis
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| - | *Recombination
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| - | *…
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| - | <br>
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| | | | |
| - | ==Project Details==
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| - | <br>
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| - | ==The Experiments==
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| - | <br>
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| - | ==Results==
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| - | <br>
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Latest revision as of 12:17, 12 July 2009
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