Team:EPF-Lausanne

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{{EPF-Lausanne09}}
{{EPF-Lausanne09}}
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[[Image:Mainpage.jpg|center]]
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<br><br>
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==Concept==
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<br>
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<div style="text-align:justify;">
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[[Image:LovTAP_dimer.png|right|300px|thumb|LovTAP dimer bound to DNA]]
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Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
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<div CLASS="epfltrick">__TOC__
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For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.
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[[Image:Mainpage.jpg|700 px|center]]
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The results clearly show that this light-induced gene switch tool works ''in vivo'', demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.
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[http://www.ubs.ch Main sponsor:]
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[[Image:Logo_UBS.jpg|link "www.ubs.com"|200 px|center]]
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==Last News==
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<br><br><br><br>
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|-
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! scope=col | [[Image:Logo_MerckSerono.png|80 px]]
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! scope=col | [[Image:Logo_Novartis.png|100 px]]
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! scope=col | [[Image:Logo_Syngenta.png|80 px]]
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! scope=col | [[Image:Logo_UBS.jpg|200 px]]
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! scope=col | [[Image:Logo_ciba.jpg|80 px]]
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! scope=col | [[Image:Logo_nikon.jpg|60 px]]
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! scope=col | [[Image:Logo_tecan.gif|80 px]]
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|}
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----
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</div>
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'''Keep track with what we did so far'''
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''(12.07.09)''
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:This first week of wetlab we have done the following things
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*Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
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*Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
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*Orderd and received the primers needed for the PCR of LovTAP
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*Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
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*Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about this parts) into competent E.Coli
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*Fused the two BioBricks "LacI" and "term"
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*Digested the LovTAP PCR products and RBS part
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==Project Abstact==
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</div><div CLASS="epfl09bouchon"></div>
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Latest revision as of 22:45, 21 October 2009



Mainpage.jpg



Concept


LovTAP dimer bound to DNA

Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.

For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.

The results clearly show that this light-induced gene switch tool works in vivo, demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.





Logo MerckSerono.png Logo Novartis.png Logo Syngenta.png Logo UBS.jpg Logo ciba.jpg Logo nikon.jpg Logo tecan.gif

Locations of visitors to this page

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