Team:Gaston Day School/19 June 2009

From 2009.igem.org

(Difference between revisions)
(New page: <html> <du> <dt>High eff. e. Coli for (RFP, stop codon, constit. RFP)</dt> <dt>Thaw e. Coli till ice disappears</dt> <dt>Pippete 50x into tube X3</dt> <dt>Add and mix plasmid X3</dt> <dt>S...)
 
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<html>
<du>
<du>
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<dt>High eff. e. Coli for (RFP, stop codon, constit. RFP)</dt>
+
<dt>High eff. E. coli for (RFP, stop codon, constit. RFP)</dt>
-
<dt>Thaw e. Coli till ice disappears</dt>
+
<dt>Thaw E. coli till ice disappears</dt>
<dt>Pippete 50x into tube X3</dt>
<dt>Pippete 50x into tube X3</dt>
<dt>Add and mix plasmid X3</dt>
<dt>Add and mix plasmid X3</dt>
<dt>Suspend the well in 15x TE</dt>
<dt>Suspend the well in 15x TE</dt>
-
<dt>Add 5x of plasmid to e. Coli</dt>
+
<dt>Add 5x of plasmid to E. coli</dt>
-
<dt>Place oni ce for 30 mins</dt>
+
<dt>Place on ice for 30 mins</dt>
<dt>Heat shock at 42C for 30 sec</dt>
<dt>Heat shock at 42C for 30 sec</dt>
<dt>Ice for 5 mins</dt>
<dt>Ice for 5 mins</dt>

Latest revision as of 14:25, 20 July 2009

High eff. E. coli for (RFP, stop codon, constit. RFP)
Thaw E. coli till ice disappears
Pippete 50x into tube X3
Add and mix plasmid X3
Suspend the well in 15x TE
Add 5x of plasmid to E. coli
Place on ice for 30 mins
Heat shock at 42C for 30 sec
Ice for 5 mins
Add 950x SOC
Place in 37C for 60 mins
Spin 5 mins
Pipette out SOC
Resuspend in 150X SOC
Spread 50x00x onto plates

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