Team:Gaston Day School/22 June 2009
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(Difference between revisions)
(New page: <html> <du> <dt>High eff. EcoRI: RFP, constit. RFP</dt> <dt>Thaw e. Coli till ice disappears</dt> <dt>Pipette 100x into tube (X2)</dt> <dt>Add 10x of plasmid to e. Coli</dt> <dt>Place on i...) |
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<dt>Washed pellet in 70% EtOH 300x</dt> | <dt>Washed pellet in 70% EtOH 300x</dt> | ||
<dt>Spin 5 mins</dt> | <dt>Spin 5 mins</dt> | ||
+ | <dt>pst = B#3</dt> | ||
+ | <dt>Spe and Xba = B#4</dt> | ||
<dt>Resuspend 30x TE</dt> | <dt>Resuspend 30x TE</dt> | ||
<dt>Pst = B #3</dt> | <dt>Pst = B #3</dt> | ||
<dt>Spe and ba = B#4</dt> | <dt>Spe and ba = B#4</dt> | ||
- | <dt></dt> | + | <dt>DIGEST</dt> |
- | <dt></dt> | + | <dt>10x DNA</dt> |
- | <dt> | + | <dt>3x Buffer/B#3/B#4</dt> |
- | + | <dt>15x dH2O</dt> | |
- | + | <dt>2x Eco RI/Pst/SPe/Xba</dt> | |
- | <dt></dt> | + | |
- | <dt> | + | |
- | + |
Latest revision as of 13:53, 20 July 2009