Team:Wash U/Calendar-Home/14 July 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 16:43, 15 July 2009

Tuesday, July 14, 2009

-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC

Gel #1: Silver digestion protocol

1) Ladder

2) OmpC + pSB1A2 plasmid (2187 bp)

3) OmpC + pSB1A2 plasmid

4) GFP (876 bp)

5) GFP

6) Ladder

Gel #2: BioBrick (Triligation) digestion protocol

1) Ladder

2) OmpC (108 bp)

3) OmpC

4) Destination plasmid, pSB1K3 (2206 bp)

5) Dest. plasmid, pSB1K3

6) GFP (876 bp)

7) GFP

8) Ladder

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@@ Line 10: / Line 10: @@
 -Started cultures of dbcOmega in LB+Strep in lieu of ATCC <br>
-Gel #1: Silver
+Gel #1: Silver digestion protocol
 ) Ladder
-) #1 OmpC
+) OmpC + pSB1A2 plasmid (2187 bp)
-) #1 OmpC
+) OmpC + pSB1A2 plasmid
-) #1 GFP
+) GFP (876 bp)
-) #1 GFP
+) GFP
 ) Ladder
-Gel #2: BioBrick
+Gel #2: BioBrick (Triligation) digestion protocol
 ) Ladder
-) #2 OmpC
+) OmpC (108 bp)
-) #2 OmpC
+) OmpC
-) #2 Destination plasmid (pSB1K3)
+) Destination plasmid, pSB1K3 (2206 bp)
-) #2 Dest. plasmid (pSB1K3)
+) Dest. plasmid, pSB1K3
-) #2 GFP
+) GFP (876 bp)
-) #2 GFP
+) GFP
 ) Ladder
@@ Line 45: / Line 45: @@
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-[[Team:Wash_U/Calendar-Home/9 July 2009|Previous entry]]</div>
+[[Team:Wash_U/Calendar-Home/13 July 2009|Previous entry]]</div>
-  [[Team:Wash_U/Calendar-Home/14 July 2009|Next entry]]</div>
+  [[Team:Wash_U/Calendar-Home/15 July 2009|Next entry]]</div>
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