Team:Sheffield

From 2009.igem.org

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Latest revision as of 17:58, 21 October 2009

Home	Team	Project	Further Work	Modeling	Notebook

SWITCHED ON !!!

For the second time running [http://www.sheffield.ac.uk/ University of Sheffield] is proud to undertake another remarkable iGEM journey. The team constitute of three members specializing in different fields of engineering such as biomedical and system control.

Project Description

By modifying E.coli so that it can use a phytochrome- with a light receptor- from cyanobacteria as a trigger of protein generation. This pathway is controlled by a certain wavelength of red light, acting as a system switch for lacZ production. LacZ can react with substrate X-gal and form a blue precipitate as a reporter. However, other reporter genes can be attached to the lacZ gene, so different reporters can be expressed. From the fact that this mechanism is sensitive to a certain wavelength of light, we hope to create a system that can be sensitive to various wavelengths and hence triggering different protein generation. Through this the E.coli can become a wavelength sensor; a different wavelength can trigger a different production of protein, for example various types of fluorescent protein, giving a different a colour-scaled indication of the wavelength of the environment around the E.coli.

Our aim is to design an E.coli system that is sensitive to multiple wavelengths of light and therefore produce a colour indication of the specific wavelength it is exposed to. A pictorial description is shown below:

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+{|body style="background-color:#7CFC00;"
+|[[Image:SHEF LOGO.png|650px|right|border]]
+|-
+|
+|}
+{| style="color:#FFFF00;background-color:#ADFF2F;"  cellpadding="1" cellspacing="1" width="100%" align="center"
+!align="center"|[[Team:Sheffield/Home|Home]]
+!align="center"|[[Team:Sheffield/Team|Team]]
+!align="center"|[[Team:Sheffield/Project|Project]]
+!align="center"|[[Team:Sheffield/Further Work|Further Work]]
+!align="center"|[[Team:Sheffield/Modeling|Modeling]]
+!align="center"|[[Team:Sheffield/Notebook|Notebook]]
+|}
+{|body style="background-color:#7CFC00;"
+|
-{|align="justify"
+[[Image:title.png|left|200px|border]]
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+===SWITCHED ON !!!===
-|[[Image:Sheffield_logo.jpg|50px|right|frame]]
+For the second time running [http://www.sheffield.ac.uk/ University of Sheffield] is proud to undertake another remarkable iGEM journey. The team constitute of three members specializing in different fields of engineering such as biomedical and system control.
-|-
-|
-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
-|[[Image:Team.png|right|frame|Your team picture]]
-|-
-|
-|align="center"|[[Team:Sheffield | Team Example]]
-|}
+===Project Description===
+[[Image:Sheffield_logo.jpg|right|150px|border]]
+By modifying E.coli so that it can use a phytochrome- with a light receptor- from cyanobacteria as a trigger of protein generation. This pathway is controlled by a certain wavelength of red light, acting as a system switch for lacZ production. LacZ can react with substrate X-gal and form a blue precipitate as a reporter. However, other reporter genes can be attached to the lacZ gene, so different reporters can be expressed. From the fact that this mechanism is sensitive to a certain wavelength of light, we hope to create a system that can be sensitive to various wavelengths and hence triggering different protein generation. Through this the E.coli can become a wavelength sensor; a different wavelength can trigger a different production of protein, for example various types of fluorescent protein, giving a different a colour-scaled indication of the wavelength of the environment around the E.coli.
+Our aim is to design an E.coli system that is sensitive to multiple wavelengths of light and therefore produce a colour indication of the specific wavelength it is exposed to. A pictorial description is shown below:
+[[Image:intropic.png|center|600px|border]]
+==Sponsors==
+[[Image:sponsor.png|left|500px|border]]
+[[Image:bbsrc.png|center|200px|border]]
+[[Image:chelsi.png|left|500px|border]]
+[[Image:epsrc.png|center|120px|border]]
+Special thanks to:
+Prof Philip Wright, Prof Visakan Kadirkamanathan, Prof Alan Matthews, Dr Jagroop Pandhal, Dr Josselin Noirel, Tara
+Baldacchino and everyone in the bioincubator.
-<!--- The Mission, Experiments --->
-{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
-!align="center"|[[Team:Sheffield|Home]]
-!align="center"|[[Team:Sheffield/Team|The Team]]
-!align="center"|[[Team:Sheffield/Project|The Project]]
-!align="center"|[[Team:Sheffield/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:Sheffield/Modeling|Modeling]]
-!align="center"|[[Team:Sheffield/Notebook|Notebook]]
 |}
-(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')