Team:Paris/Protocols

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Protocols#top | Protocols]] > [[Team:Paris/Protocols#bottom | Main]]
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== Protocols ==
 
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iGEM paris 2007 protocols link :
 
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content :
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==Main==
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Growing bacteria in liquid medium
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Preparing growth media
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exemple:Making 10 petri dish (LB+erythromycin+citrate+DAP)
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                Solid M9 Minimum Medium
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Preparation de gel d'agarose
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Chemical transformation
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Recombineering/Lambda red-mediated gene replacement
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Miniprep
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Fluorescent single cells visualisation
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Digestion reactions
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<div id="middle-side"><center>
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Protocols#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/ProtocolsA#bottom"> Microscope Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsB#bottom"> Adapted Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Protocols_Culture#bottom"> Culture Protocols</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/ProtocolsMB#bottom"> Molecular biology</a>
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===1. Microscopy===
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* [[Team:Paris/ProtocolsA#Protocols suggested | Protocols suggested]]
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*[http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]
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===2. Adapted Protocols===
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* DNA extraction
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* [[Team:Paris/ProtocolsB#Adaptation of PureYield&trade; Plasmid Miniprep System (Promega) |Mini prep ]]
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*  Gel/PCR Purification
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===3. Culture protocols===
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* Over Night
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*[[Team:Paris/Protocols_Culture#Bacterial transformation | Bacterial transformation]]
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* [[Team:Paris/Protocols_Culture#Competent bacteria | Competent bacteria]]
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**[[team:Paris/Protocols_Culture#CaCl2a|CaCl<sub>2</sub> (iGEM Paris2007)]]
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**[[team:Paris/Protocols_Culture#CaCl2b|CaCl<sub>2</sub> (2nd Protocol)]]
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**[[team:Paris/Protocols_Culture#RbCl|RbCl]]
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* [[team:Paris/Protocols_Culture#Glycerol stock|Glycerol stock]]
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*[[team:Paris/Protocols_Culture#Ferric citrate growth medium|Ferric citrate growth medium]]
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===4. Molecular biology===
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*[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]]
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*[[Team:Paris/ProtocolsMB#PCR colonies|PCR colony]]
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* Gels
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* Digestion
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* Ligation

Latest revision as of 16:35, 20 October 2009

iGEM > Paris > Protocols > Main


Contents

Main

1. Microscopy

  • Protocols suggested
  • [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]

2. Adapted Protocols

3. Culture protocols

4. Molecular biology

Retrieved from "http://2009.igem.org/Team:Paris/Protocols"