Team:Paris/Freezer

From 2009.igem.org

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer#bottom | Main]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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== PCR products ==
 
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{|
 
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|- style="background: #ccccff;"
 
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! colspan="5" style="background: #ccccff;"|PCR products
 
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|- style="background: #ccccff; text-align: center;"
 
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|width=5%| Number
 
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|width=10%| Name
 
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|width=12.5%| Oligo F
 
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|width=12.5%| Oligo R
 
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|width=10%| Matrix
 
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|width=5%| Length
 
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|width=25%| Comments
 
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|width=5%| Product Left ?
 
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|width=5%| []ng/µL
 
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|width=5%| Date
 
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|}
 
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http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
 
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http://parts.mit.edu/images/partbypart/icon_regulatory.png
 
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http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
 
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http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
 
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http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
 
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http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
 
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http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
 
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http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
 
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==Freezer==
<html>
<html>
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</div>
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<style type="text/css">
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<div id="paris_content_boxtop">
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#left-side {
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</div>
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    position: absolute;
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<div id="paris_content">
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    height: 23px;
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</html>
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    width: 30px;
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    top: 0px;
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    left: 10px;
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    margin-top:10px;
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    padding-top: 7px;
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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    z-index:4;
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}
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== Plasmids: MiniPrep Products ==
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#middle-side {
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{|
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    height: 25px;
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|- style="background: #ccccff;"
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    width: 650px;
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! colspan="5" style="background: #ccccff;" | Plasmids: MiniPrep Products
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    position: absolute;
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|- style="background: #ccccff; text-align: center;"
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    top: 0px;
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|width=5%| Number
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    left: 30px;
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|width=25%| Name
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    margin-top:10px;
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|width=50%| Description
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    padding-top: 5px;
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|width=15%| Products in use
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    background: #dadada;
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|width=5%| Date
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    z-index:5;
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|}
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}
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#right-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    margin-top:10px;
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    padding-top: 7px;
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    top: 0px;
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    left: 670px;
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    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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    z-index:4;
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}
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<html>
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a.menu_sub {
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</div>
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    padding-left: 7px;
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<div id="paris_content_boxtop">
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    padding-right: 7px;
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</div>
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}
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<div id="paris_content">
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</html>
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== Digestion Products ==
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a.menu_sub_active {
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<br>
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    padding-left: 7px;
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<font size="4">PURPLE = Vector </font><br>
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    padding-right: 7px;
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<font size="4">GREEN = Insert </font><br>
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    color:#b0310e;
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{|
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    font-weight:bold;
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|- style="background: #ccccff;"
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}
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! colspan="5" style="background: #ccccff;" | Digestion Products
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</style>
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|- style="background: #ccccff; text-align: center;"
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<div id="left-side"></div>
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|width=5%| Number
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<div id="middle-side"><center>
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|width=20%| Product Name
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
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|width=20%| Matrix Name
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
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|width=5%| Enzyme 1
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
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|width=5%| Enzyme 2
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
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|width=5%| Size
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
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|width=30%| Description
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
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|width=5%| Product Left
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</center>
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|width=5%| []ng/µL
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|width=5%| Date
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|}
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<html>
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</div>
</div>
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<div id="paris_content_boxtop">
 
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</div>
 
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<div id="paris_content">
 
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</html>
 
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== Unexpected digestion results ==
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<div id="right-side"></div>
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{|
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|- style="background: #ccccff;"
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! colspan="7" style="background: #ccccff;" | Unexpected results
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=20%| Matrix Name
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|width=5%| Enzyme 1
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|width=5%| Enzyme 2
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|width=5%| Expected Size
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|width=55%| Description
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|width=5%| Date
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|}
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<html>
 
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</div>
 
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<div id="paris_content_boxtop">
 
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</div>
 
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<div id="paris_content">
 
</html>
</html>
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== Strains (glycerol stock) ==
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<br>
{|
{|
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|- style="background: #ccccff;"
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|- style="background: #c5c5c5;"
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! colspan="5" style="background: #ccccff;" | Strains glycerol stock
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! colspan="8" style="background:#c5c5c5; text-align: center" | Color code for sticker
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|- style="background: #ccccff; text-align: center;"
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|- style="background: #0d3e99; text-align: center; color:black;"
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|width=5%| Number
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|width=10% style="background: #FF0000;"| Vector
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|width=25%| Name
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|width=10% style="background: #FFFF00;"| Insert
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|width=55%| Description
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|width=10% style="background: #660099;"| PCR product
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|width=5%| Date
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|width=10% style="background: #FF6600;"| Plasmid
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|- style="background: #ccccff; text-align: center;"
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|width=10% style="background: #FFFFFF;"| Strains
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|[https://2009.igem.org/Team:Paris/Strains#Strain_1  S1]
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|width=10% style="background: #006400;"| Kanamycin
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|DH5-alpha
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|width=10% style="background: #3333FF;"| Ampicilin
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|DH5-alpha E. Coli strain for chimically competent
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|width=10% style="background: #FF0066;"| Chloranphenicol
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|[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
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|- style="background: #ccccff; text-align: center;"
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|[https://2009.igem.org/Team:Paris/Strains#Strain_2  S2]
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|K12 MG 1655 delta recA::Kan
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|Standard E.Coli strain, Kan resistance
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|[https://2009.igem.org/Team:Paris/20_July_2009 07/20]
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|- style="background: #ccccff; text-align: center;"
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|[https://2009.igem.org/Team:Paris/Strains#Strain_3  S3]
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|W3110 delta nlp1
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|From Daniel Rodriguez, Kan resistance
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|[https://2009.igem.org/Team:Paris/20_July_2009 07/23]
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|}
|}

Latest revision as of 15:29, 19 October 2009

iGEM > Paris > Freezer > Main


Freezer


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Retrieved from "http://2009.igem.org/Team:Paris/Freezer"