Team:Virginia/23 July 2009

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*PCRed HA tag onto PCS
*PCRed HA tag onto PCS
*Ran gel of tagged PCS-HA
*Ran gel of tagged PCS-HA
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**Photo to be added
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**[[Image:PCS-HA.JPG‎]]
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**Product of PCR on PCS in attempt to add HA tag
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**Results indicate that there was no PCR product.

Latest revision as of 18:44, 27 July 2009

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Overnight Results

Today

  • Ligated A09-10
    • ABP-HA E,P
    • pSB1C3 E,P
    • Versions 1, 2, and 3 reflect different ratios of vector to insert. 1:2, 1:6, 1:1 respectively.
  • Transformed A09-10 into DH5alphas
  • PCRed HA tag onto PCS
  • Ran gel of tagged PCS-HA
    • PCS-HA.JPG
    • Product of PCR on PCS in attempt to add HA tag
    • Results indicate that there was no PCR product.
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