Team:Warsaw/Calendar-Main/24 July 2009

From 2009.igem.org

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<ul>
<ul>
<li>Despite the fact that all transferred colonies turned more or less blue the purification was carried out using the Plasmid Mini kit (A&A Biotechnology) according to the manufacturers protocol.</li>
<li>Despite the fact that all transferred colonies turned more or less blue the purification was carried out using the Plasmid Mini kit (A&A Biotechnology) according to the manufacturers protocol.</li>
-
<li>The digest mix was prepared as fallows: 10&mu;l of purified plasmid, 2&mu;l Tango buffer (Fermentas), 1&mu;l SpeI enzyme, 1&mu;l XbaI enzyme, the solution was topped up with H2O to the final volume of 20&mu;l.</li>
+
<li>The digest mix was prepared as follows:  
 +
<pre>10&mu;l of purified plasmid  
 +
2&mu;l Tango buffer (Fermentas)  
 +
1&mu;l SpeI enzyme  
 +
1&mu;l XbaI enzyme</pre>
 +
The solution was topped up with H2O to the final volume of 20&mu;l.</li>
<li>The digest was incubated for 3h at 37&deg;C.
<li>The digest was incubated for 3h at 37&deg;C.
<li>The results were visualised with gel electrophoresis on 1% agarose gel.
<li>The results were visualised with gel electrophoresis on 1% agarose gel.
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<li>Gel Electrophoresis:</li>
<li>Gel Electrophoresis:</li>
</ul>
</ul>
-
<p>Sukcesy
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<img src=https://static.igem.org/mediawiki/2009/5/57/2009.07.24.jpg />
-
<p>&nbsp;
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<p>From left:
-
<p>Nigdy mi nie odmówiły
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<ol>
-
<p>Te, które mi się śniły.
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<li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
-
<p>(Jan Sztaudynger)
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<li>#1 Digest</li>
 +
<li>#2 Digest</li>
 +
<li>#3 Digest</li>
 +
<li>#4 Digest</li>
 +
<li>#5 Digest</li>
 +
</ol>
 +
<p>NOTE: All of the samples consisted of just a linear plasmid.
<br />
<br />
-
</html>
+
<p>Conclusions:</p>
 +
<ul>
 +
<li>A colony is blue for a reason.</li>
 +
</ul>
 +
<br />
 +
<h3><div style="text-align: center;">Construction of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 ">K177012</a>  operon1_part2 </div></h3>
 +
<h4>Ania</h4>
 +
<br/>
 +
<p>Tasks:</p>
 +
 +
<ul>
 +
<li>Set up 20 liquid cultires, plasmid isolation, EcorI/PstI digest.  </a> </li></ul>
 +
</html>
<html>
<html>
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<li>Isolate the plasmids from bacterial cultures and verify the effectivity of the isolation via gel electrophoresis</li>
<li>Isolate the plasmids from bacterial cultures and verify the effectivity of the isolation via gel electrophoresis</li>
<p>Methods:</p>
<p>Methods:</p>
-
<li>Plasmids weren isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described <a href="http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf" here>here</a></li>
+
<li>Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described <a href="http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf" here>here</a></li>
<li>After the isolation 1 ul of each plasmid solution was diluted to 10 ul and loaded into the 1% agarose gel</li>
<li>After the isolation 1 ul of each plasmid solution was diluted to 10 ul and loaded into the 1% agarose gel</li>
<li>Electrophoresis condition:</li></ul>
<li>Electrophoresis condition:</li></ul>
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<p>Methods:</p><ul>
<p>Methods:</p><ul>
<li>Digest of isolate plasmids using EcoRI and PstI</li>
<li>Digest of isolate plasmids using EcoRI and PstI</li>
-
<ul><li>Reaction mixture composition: 0.5 &mu;l purified plasmid DNA product, 0.5 &mu;l EcoRI (Fermentas),0.5 &mu;l PstI (Fermentas), 2 &mu;l Buffer Tango (Fermentas), 16.5 &mu;l MQ water</li></ul>
+
<ul><li>Reaction mixture composition:  
 +
<pre<0.5 &mu;l purified plasmid DNA product  
 +
0.5 &mu;l EcoRI (Fermentas)
 +
0.5 &mu;l PstI (Fermentas)  
 +
2 &mu;l Buffer Tango (Fermentas)  
 +
16.5 &mu;l MQ water</pre></li></ul>
<li>The reaction was performed three hours and it was subsequently inactivated via heating in 80&deg;C for 20 minutes.</li>
<li>The reaction was performed three hours and it was subsequently inactivated via heating in 80&deg;C for 20 minutes.</li>
<li>In the next step reaction mixtures were loaded into the agarose gel to analize restriction pattern of the plasmids</li>
<li>In the next step reaction mixtures were loaded into the agarose gel to analize restriction pattern of the plasmids</li>
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</html>
</html>
 +
<html>
 +
<h3><div style="text-align: center;">Preparation of the electrocompetent bacteria</div></h3>
 +
<h4>Marcin</h4>
 +
<br/>
 +
<p>Task 1:</p>
 +
<ul>
 +
<li>Preparation of electrocompetent E.coli strain TOP10</li>
 +
<p>Methods:</p>
 +
<ul>
 +
<li>detailed ptotocol of preparation</li><ul>
 +
<ol><li>Prepare new bacterial culture - add 10 ml of previously prepared bacterial inoculum to 1 l of LB liquid medium.</li>
 +
<li>Incubate the culture in 37&deg;C until the absorbance of the bacterial solution will be at least 0.5
 +
<li>Cooled tne bacteria on ice for at least 25 minutes.</li>
 +
<li>Centrifuge the culture for 15 minutes at 4000g for 4&deg;C minutes. The temperarure must be below 5&deg;C.</li>
 +
<li>Carefully remove the supernatant and resuspend the bacteria in 1 l of cold water.</li>
 +
<li>Centrifuge the bacteria in the same manner as previously.</li>
 +
<li>Resuspend the bacteria in 0.5 l of cold water.</li>
 +
<li>Repeated the centrifuge procedure.</li
 +
<li>Resuspend carefully last formed pellet in 10% solution of the glycerole</li>
 +
<li>Centrifuge the culture for 15 minutes at 4000g for 4&deg;C minutes. The temperarure must be below 5&deg;C</li>
 +
<li>Resuspent the bacterial cells in 3 ml of 10% solution of the glycerole and prepare the aliquots of the bacteria which all contains about 200 &mu;l of the solution</li>
 +
<li>Freeze the bacteria in liquid nitrogen.</li></ol>
-
 
+
</html>

Latest revision as of 16:17, 26 September 2009


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Cloning of the mgtc promoter into the pKSII+ plasmid

Kamil


Tasks:

  • Plasmid purification
  • Control digest

Methods:

  • Despite the fact that all transferred colonies turned more or less blue the purification was carried out using the Plasmid Mini kit (A&A Biotechnology) according to the manufacturers protocol.
  • The digest mix was prepared as follows:
    10μl of purified plasmid 
    2μl Tango buffer (Fermentas) 
    1μl SpeI enzyme 
    1μl XbaI enzyme
    The solution was topped up with H2O to the final volume of 20μl.
  • The digest was incubated for 3h at 37°C.
  • The results were visualised with gel electrophoresis on 1% agarose gel.

Results:

  • Gel Electrophoresis:

From left:

  1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
  2. #1 Digest
  3. #2 Digest
  4. #3 Digest
  5. #4 Digest
  6. #5 Digest

NOTE: All of the samples consisted of just a linear plasmid.

Conclusions:

  • A colony is blue for a reason.

Construction of K177012 operon1_part2

Ania


Tasks:

  • Set up 20 liquid cultires, plasmid isolation, EcorI/PstI digest.

Assembly of endosomal detection operon

Marcin


Task 1:

  • Isolate the plasmids from bacterial cultures and verify the effectivity of the isolation via gel electrophoresis
  • Methods:

  • Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
  • After the isolation 1 ul of each plasmid solution was diluted to 10 ul and loaded into the 1% agarose gel
  • Electrophoresis condition:

voltage - 70V

time - 30 min

Task 2:

  • Digest of isolate plasmids with ligated biobricks to verify the success of ligation

Methods:

  • Digest of isolate plasmids using EcoRI and PstI
    • Reaction mixture composition:
  • The reaction was performed three hours and it was subsequently inactivated via heating in 80°C for 20 minutes.
  • In the next step reaction mixtures were loaded into the agarose gel to analize restriction pattern of the plasmids

  • Comment:

    The procedure of digest was incorrect due to use Tango Buffer - EcoRI need specific buffer and it may do not work correctly in this buffer. It is obligated to repeat the procedure using modified set on restriction enzymes.

    Preparation of the electrocompetent bacteria

    Marcin


    Task 1:

    • Preparation of electrocompetent E.coli strain TOP10
    • Methods:

      • detailed ptotocol of preparation
          1. Prepare new bacterial culture - add 10 ml of previously prepared bacterial inoculum to 1 l of LB liquid medium.
          2. Incubate the culture in 37°C until the absorbance of the bacterial solution will be at least 0.5
          3. Cooled tne bacteria on ice for at least 25 minutes.
          4. Centrifuge the culture for 15 minutes at 4000g for 4°C minutes. The temperarure must be below 5°C.
          5. Carefully remove the supernatant and resuspend the bacteria in 1 l of cold water.
          6. Centrifuge the bacteria in the same manner as previously.
          7. Resuspend the bacteria in 0.5 l of cold water.
          8. Repeated the centrifuge procedure.
          9. Resuspend carefully last formed pellet in 10% solution of the glycerole
          10. Centrifuge the culture for 15 minutes at 4000g for 4°C minutes. The temperarure must be below 5°C
          11. Resuspent the bacterial cells in 3 ml of 10% solution of the glycerole and prepare the aliquots of the bacteria which all contains about 200 μl of the solution
          12. Freeze the bacteria in liquid nitrogen.



          April
          MTWTFSS
              [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_April_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_April_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_April_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_April_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_April_2009 5]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_April_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_April_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_April_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_April_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_April_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_April_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_April_2009 12]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_April_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_April_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_April_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_April_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_April_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_April_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_April_2009 19]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_April_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_April_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_April_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_April_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_April_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_April_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_April_2009 26]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_April_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_April_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_April_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_April_2009 30]
          May
          MTWTFSS
                  [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_May_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_May_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_May_2009 3]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_May_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_May_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_May_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_May_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_May_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_May_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_May_2009 10]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_May_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_May_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_May_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_May_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_May_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_May_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_May_2009 17]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_May_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_May_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_May_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_May_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_May_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_May_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_May_2009 24]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_May_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_May_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_May_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_May_2009 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_May_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
          June
          MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/2_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/13_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/14_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 14]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/15_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_June_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/17_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_June_2009 18] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_June_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_June_2009 28]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_June_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_June_2009 30]
          July
          MTWTFSS
              [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_July_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_July_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_July_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_July_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009 5]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_July_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_July_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_July_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_July_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_July_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_July_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_July_2009 12]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_July_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_July_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_July_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_July_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_July_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_July_2009 19]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_July_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_July_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_July_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_July_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_July_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_July_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_July_2009 26]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_July_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_July_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_July_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_July_2009 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_July_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]
          August
          MTWTFSS
                    [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/1_August_2009&preload=Team:Warsaw/NotebookPreload&action=edit 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_August_2009 2]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_August_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_August_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_August_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_August_2009 6] [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_August_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_August_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_August_2009 9]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_August_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_August_2009 11] [http://2009.igem.org/Team:Warsaw/Calendar-Main/12_August_2009 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_August_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_August_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_August_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_August_2009 16]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_August_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_August_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_August_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_August_2009 20] [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_August_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_August_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_August_2009 23]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_August_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_August_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_August_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_August_2009 27] [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_August_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_August_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_August_2009 30]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/31_August_2009 31]
          September
          MTWTFSS
            [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_September_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_September_2009 2] [http://2009.igem.org/Team:Warsaw/Calendar-Main/3_September_2009 3] [http://2009.igem.org/Team:Warsaw/Calendar-Main/4_September_2009 4] [http://2009.igem.org/Team:Warsaw/Calendar-Main/5_September_2009 5] [http://2009.igem.org/Team:Warsaw/Calendar-Main/6_September_2009 6]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/7_September_2009 7] [http://2009.igem.org/Team:Warsaw/Calendar-Main/8_September_2009 8] [http://2009.igem.org/Team:Warsaw/Calendar-Main/9_September_2009 9] [http://2009.igem.org/Team:Warsaw/Calendar-Main/10_September_2009 10] [http://2009.igem.org/Team:Warsaw/Calendar-Main/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_September_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_September_2009 13]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_September_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_September_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_September_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_September_2009 17] [http://2009.igem.org/Team:Warsaw/Calendar-Main/18_September_2009 18] [http://2009.igem.org/Team:Warsaw/Calendar-Main/19_September_2009 19] [http://2009.igem.org/Team:Warsaw/Calendar-Main/20_September_2009 20]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/21_September_2009 21] [http://2009.igem.org/Team:Warsaw/Calendar-Main/22_September_2009 22] [http://2009.igem.org/Team:Warsaw/Calendar-Main/23_September_2009 23] [http://2009.igem.org/Team:Warsaw/Calendar-Main/24_September_2009 24] [http://2009.igem.org/Team:Warsaw/Calendar-Main/25_September_2009 25] [http://2009.igem.org/Team:Warsaw/Calendar-Main/26_September_2009 26] [http://2009.igem.org/Team:Warsaw/Calendar-Main/27_September_2009 27]
          [http://2009.igem.org/Team:Warsaw/Calendar-Main/28_September_2009 28] [http://2009.igem.org/Team:Warsaw/Calendar-Main/29_September_2009 29] [http://2009.igem.org/Team:Warsaw/Calendar-Main/30_September_2009 30]
          October
          MTWTFSS
                [http://2009.igem.org/Team:Warsaw/Calendar-Main/1_October_2009 1] [http://2009.igem.org/Team:Warsaw/Calendar-Main/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/3_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/4_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 4]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/5_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/6_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/7_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/8_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/9_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/10_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/11_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 11]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/12_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 12] [http://2009.igem.org/Team:Warsaw/Calendar-Main/13_October_2009 13] [http://2009.igem.org/Team:Warsaw/Calendar-Main/14_October_2009 14] [http://2009.igem.org/Team:Warsaw/Calendar-Main/15_October_2009 15] [http://2009.igem.org/Team:Warsaw/Calendar-Main/16_October_2009 16] [http://2009.igem.org/Team:Warsaw/Calendar-Main/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 18]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/19_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/20_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/21_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/22_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/23_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/25_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 25]
          [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/26_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/27_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/28_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/29_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/30_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/31_October_2009&preload=Team:Warsaw/NotebookPreload&action=edit 31]