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Team:Virginia/27 July 2009
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ThaddeusWebb (Talk | contribs) (New page: {{Template:VirginiaNotebook}} ==Overnight Results== All platings blank again ==Today==) |
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==Overnight Results== | ==Overnight Results== | ||
| - | All platings blank again | + | *All platings blank again |
| + | |||
==Today== | ==Today== | ||
| + | *PCR to add HA tag to ABP | ||
| + | **1ul of ABP DNA @ 422ng/ul | ||
| + | **3 different MgCl2 concentrations: 1, 2, 4 mM | ||
| + | **Control w/o ABP DNA | ||
| + | **2 cycles with 58C annealing temp, then 28 cycles with 65C annealing temp | ||
| + | **74C extension step, 10 sec | ||
| + | **[[Image:Vgem072709.jpg]] | ||
| + | **Ladder, No template DNA, Template DNA & 1mM MgCl2,...& 2mM MgCl2,...& 4mM MgCl2 | ||
Latest revision as of 19:11, 27 July 2009
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Overnight Results
- All platings blank again
Today
- PCR to add HA tag to ABP
- 1ul of ABP DNA @ 422ng/ul
- 3 different MgCl2 concentrations: 1, 2, 4 mM
- Control w/o ABP DNA
- 2 cycles with 58C annealing temp, then 28 cycles with 65C annealing temp
- 74C extension step, 10 sec
- Ladder, No template DNA, Template DNA & 1mM MgCl2,...& 2mM MgCl2,...& 4mM MgCl2
