Team:Paris/Protocols PCRqload

From 2009.igem.org

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==PCR quick load Taq==
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==PCR with Quick load Taq2x Master Mix==
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*Mix for 50µl final:
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**14µL H2O
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<p>Préparation de la PCR avec un Vf=50µL:</p>
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**25µL Taq Quick load Master Mix 2X
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<p>-1µL Matrice</p>
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**5µL Oligo Fw (0,2µM)
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<p>-14µL H2O</p>
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**5µL Oligo Rw (0,2µM)
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<p>-5µ Oligo Fw (à 0,2M)</p>
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**1µL Matrice (DNA)
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<p>-5µL Oligo Rw (à 0,2M)</p>
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<p>-25µL Tampon+Taq (Taq quick load master mix 2X)</p>
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Latest revision as of 00:02, 31 July 2009

PCR with Quick load Taq2x Master Mix

  • Mix for 50µl final:
    • 14µL H2O
    • 25µL Taq Quick load Master Mix 2X
    • 5µL Oligo Fw (0,2µM)
    • 5µL Oligo Rw (0,2µM)
    • 1µL Matrice (DNA)

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