User:Annlesn

From 2009.igem.org

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[[media:7_22_09_and_7_23_09_Transformation_of_RBS_for_Pu_and_Killer_genes.pdf| 7/22/09 and 7/23/09]]
[[media:7_22_09_and_7_23_09_Transformation_of_RBS_for_Pu_and_Killer_genes.pdf| 7/22/09 and 7/23/09]]
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[[media:7_24_09_Miniprep_and_Digest_of_Pu_promoter_and_killer_genes.pdf‎ | 7/22/09 and 7/23/09]]
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*Comp cell Protocol
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*Protocol for getting biobricks from the registry
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*Transformation Protocol
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*Transformation of RBS for Pu promoter and killer genes from the biobricks
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[[media:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette.pdf‎ | 7/22/09 and 7/23/09]]
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[[media:7_24_09_Miniprep_and_Digest_of_Pu_promoter_and_killer_genes.pdf‎ | 7/24/09]]
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[[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf‎| 7/22/09 and 7/23/09]]
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*Miniprep protocol
 +
*Miniprep of RBS for Pu promoter and killer genes
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[[media:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette.pdf| 7/25/09]]
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*Nanodrop protocol
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*Digestion protocol
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*DNA concentrations for Pu promoter and killer genes
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*Digestion of RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 with EcoRI and XbaI
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[[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf‎| 7/27/09]]
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[[Image:7_24_09_Miniprep_and_Digest_of_Pu_promoter_and_killer_genes.pdf‎ |200px|thumb|left|alt text]]
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*Electrophoresis protocol
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*Gel of digested RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008
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[[Image:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette.pdf‎ |200px|thumb|left|alt text]]
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*Sodium Benzoate Minimal Plate Recipe
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*Plates for Pu promoter colony PCR
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[[Image:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf‎|200px|thumb|left|alt text]]
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*Starting up Pu luciferase stab from the registry

Latest revision as of 23:08, 28 July 2009

Ann's IGEM Notebook

7/22/09 and 7/23/09

  • Comp cell Protocol
  • Protocol for getting biobricks from the registry
  • Transformation Protocol
  • Transformation of RBS for Pu promoter and killer genes from the biobricks

7/24/09

  • Miniprep protocol
  • Miniprep of RBS for Pu promoter and killer genes

7/25/09

  • Nanodrop protocol
  • Digestion protocol
  • DNA concentrations for Pu promoter and killer genes
  • Digestion of RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 with EcoRI and XbaI

7/27/09

  • Electrophoresis protocol
  • Gel of digested RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008
  • Sodium Benzoate Minimal Plate Recipe
  • Plates for Pu promoter colony PCR
  • Starting up Pu luciferase stab from the registry
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