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User:Annlesn
From 2009.igem.org
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[[media:7_22_09_and_7_23_09_Transformation_of_RBS_for_Pu_and_Killer_genes.pdf| 7/22/09 and 7/23/09]] | [[media:7_22_09_and_7_23_09_Transformation_of_RBS_for_Pu_and_Killer_genes.pdf| 7/22/09 and 7/23/09]] | ||
| + | |||
| + | *Comp cell Protocol | ||
| + | *Protocol for getting biobricks from the registry | ||
| + | *Transformation Protocol | ||
| + | *Transformation of RBS for Pu promoter and killer genes from the biobricks | ||
[[media:7_24_09_Miniprep_and_Digest_of_Pu_promoter_and_killer_genes.pdf | 7/24/09]] | [[media:7_24_09_Miniprep_and_Digest_of_Pu_promoter_and_killer_genes.pdf | 7/24/09]] | ||
| - | [[media:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette. | + | *Miniprep protocol |
| + | *Miniprep of RBS for Pu promoter and killer genes | ||
| + | |||
| + | [[media:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette.pdf| 7/25/09]] | ||
| + | |||
| + | *Nanodrop protocol | ||
| + | *Digestion protocol | ||
| + | *DNA concentrations for Pu promoter and killer genes | ||
| + | *Digestion of RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 with EcoRI and XbaI | ||
[[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf| 7/27/09]] | [[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf| 7/27/09]] | ||
| + | |||
| + | *Electrophoresis protocol | ||
| + | *Gel of digested RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 | ||
| + | *Sodium Benzoate Minimal Plate Recipe | ||
| + | *Plates for Pu promoter colony PCR | ||
| + | *Starting up Pu luciferase stab from the registry | ||
Latest revision as of 23:08, 28 July 2009
Ann's IGEM Notebook
- Comp cell Protocol
- Protocol for getting biobricks from the registry
- Transformation Protocol
- Transformation of RBS for Pu promoter and killer genes from the biobricks
- Miniprep protocol
- Miniprep of RBS for Pu promoter and killer genes
- Nanodrop protocol
- Digestion protocol
- DNA concentrations for Pu promoter and killer genes
- Digestion of RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 with EcoRI and XbaI
- Electrophoresis protocol
- Gel of digested RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008
- Sodium Benzoate Minimal Plate Recipe
- Plates for Pu promoter colony PCR
- Starting up Pu luciferase stab from the registry
