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Virginia Commonwealth/26 May 2009
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==Tuesday 26 May 2009== | ==Tuesday 26 May 2009== | ||
| + | |||
===Results=== | ===Results=== | ||
| + | * Overnight cultures grew well. | ||
| + | ---- | ||
===Tasks=== | ===Tasks=== | ||
| + | * Inoculate 2.5 mL from each starter culture into 250mL LB media in 500ml flasks. Culture until ~0.6-1.0 A600. | ||
| + | * Freeze some glycerol seed stocks of NEB10beta and DB3.1 (2 each), concentrate rest 100x and do cold glycerol washes to make competent for electrotransformation. | ||
| + | * Freeze stocks of electrocompetent cells (-80C freezer in George's ''E. coli'' box). | ||
| + | ---- | ||
| + | ===Wetlab=== | ||
| + | * n/a | ||
Latest revision as of 19:30, 5 August 2009
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Contents |
Tuesday 26 May 2009
Results
- Overnight cultures grew well.
Tasks
- Inoculate 2.5 mL from each starter culture into 250mL LB media in 500ml flasks. Culture until ~0.6-1.0 A600.
- Freeze some glycerol seed stocks of NEB10beta and DB3.1 (2 each), concentrate rest 100x and do cold glycerol washes to make competent for electrotransformation.
- Freeze stocks of electrocompetent cells (-80C freezer in George's E. coli box).
Wetlab
- n/a
