Uppsala-Sweden/27 July 2009

From 2009.igem.org

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2009-07-27 11:15,OD measurement of Butanol at 750nm
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2009-07-27 11:15,OD measurement of Butanol at 750nm,
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pirA PCR
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pirA PCR was conducted, using
 +
1. Primer:pirA forward
 +
      pirA reverse
 +
      pirB forward
 +
  at three different template concentration,
 +
 
 +
2. Primer:pirA reverse
 +
          pirB forward
 +
at three different template concentration,
 +
Control pirB
 +
 
 +
PCR PROGRAM
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denaturing temperature 98
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denaturing time: 30s
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cycle: 5
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denaturing temperature 98
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denaturing time: 10s
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anealing temperature: 63, time 30s
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extension time:32s 
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extension temperature 72
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cycle: 30
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denaturing temperature 98
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denaturing time: 10s
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annealing temperature: 72,time 30s
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extension time:32s 
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extension temperature 72
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hold 5 min
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 +
 
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 +
     
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-- Emily

Latest revision as of 14:10, 30 July 2009

2009-07-27 11:15,OD measurement of Butanol at 750nm,

pirA PCR was conducted, using 1. Primer:pirA forward 

      pirA reverse 
      pirB forward
  at three different template concentration,

2. Primer:pirA reverse 

         pirB forward
at three different template concentration,

Control pirB

PCR PROGRAM denaturing temperature 98 denaturing time: 30s cycle: 5 denaturing temperature 98 denaturing time: 10s anealing temperature: 63, time 30s extension time:32s extension temperature 72 cycle: 30 denaturing temperature 98 denaturing time: 10s annealing temperature: 72,time 30s extension time:32s extension temperature 72 hold 5 min



-- Emily

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