Team:Harvard/Parts

From 2009.igem.org

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<h2><a href="https://2009.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2009/b/b8/Harvard2009Logo.jpg" alt="Hi Mom" width="900" height="200"></a></h2>
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<h2> Parts </h2>
 +
/***********************************************************************************************************************/
 +
        <p>
 +
 
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<b>
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<br>
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Biobrick part#1
 +
<br>
 +
Bba_K207000
 +
<br>
 +
PhyB N-terminal fragment sequence
 +
<br>
 +
Registry vector:
 +
<br>
 +
Plasmid:  pSP3K3 ,Kan resistance
 +
<br>
 +
</b>
 +
Sequence:
 +
 
 +
atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag
 +
 
 +
gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact
 +
 
 +
ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag
 +
 
 +
atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca
 +
 
 +
gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt
 +
 
 +
cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg
 +
 
 +
ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat
 +
 
 +
cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt
 +
 
 +
gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga
 +
 
 +
ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg
 +
 
 +
tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc
 +
 
 +
tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt
 +
 
 +
gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc
 +
 
 +
agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag
 +
 
 +
tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa
 +
 
 +
gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg
 +
 
 +
atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa
 +
 
 +
gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa
 +
 
 +
actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct
 +
 +
 +
<br><br><br><br><br><br><br>
 +
 +
<b>
 +
Biobrick part#2
 +
<br>
 +
Bba_K207001
 +
<br>
 +
PhyB N-terminal fragment-DBD fusion sequence
 +
<br>
 +
(GAL4 Binding Domain of pGBKT7, highlighted in bold)
 +
 +
<br>
 +
Registry vector:
 +
<br>
 +
Plasmid: pSB12A, Amp resistance
 +
<br>
 +
Subcloned from part # E0040
 +
<br>
 +
Biobrick part insert sequence:
 +
 +
<br>
 +
</b>
 +
Sequence:
 +
 
 +
atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag
 +
 
 +
gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact
 +
 
 +
ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag
 +
 
 +
atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca
 +
 
 +
gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt
 +
 
 +
cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg
 +
 
 +
ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat
 +
 
 +
cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt
 +
 
 +
gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga
 +
 
 +
ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg
 +
 
 +
tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc
 +
 
 +
tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt
 +
 
 +
gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc
 +
 
 +
agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag
 +
 
 +
tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa
 +
 
 +
gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg
 +
 
 +
atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa
 +
 
 +
gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa
 +
 
 +
actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct ATGAAGCTAC TGTCTTCTAT CGAACAAGCATGCGATATTT GCCGACTTAA AAAGCTCAAG TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG CTAGAAAGAC TGGAACAGCT ATTTCTACTG  ATTTTTCCTC GAGAAGACCT TGACATGATT TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA ACATTGAGAC AGCATAGAAT AAGTGCGACA
 +
TCATCATCGG AAGAGAGTAG TAACAAAGGT CAAAGACAGT TGACTGTATC
 +
 +
 
 +
 
 +
 
 +
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 +
<h2><a href="https://2009.igem.org/Team:Harvard/Team">Team</a></h2>
 +
<h2><p>Projects</p></h2>
 +
    <ul>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Comm">Bacteria-to-Yeast Communication</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Laser">Characterization</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/PCB">PCB Biosynthesis in Yeast</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Split">Split Luciferase</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Blackboard">Other Possible Reporters</a></li>
 +
 
 +
  </ul>
 +
<h2><p><a href="https://2009.igem.org/Team:Harvard/Parts">Parts</a></p></h2>
 +
        <h2><p><a href="https://2009.igem.org/Team:Harvard/References">References</a></p></h2>
 +
<h2><p>Lab Notebook</p></h2>
 +
    <ul>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Daily">Daily</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Protocols">Protocols</a></li>
 +
      <li><a href="https://2009.igem.org/Team:Harvard/Recipes">Recipes </a></li>
 +
    </ul>
 +
<h2><p><a href="https://2009.igem.org/Team:Harvard/Ethics">Ethics</a></h2>
 +
        <h2><p><a href="https://2009.igem.org/Team:Harvard/Safety">Safety</a></h2>
 +
        <h2><p><a href="https://2009.igem.org/Team:Harvard/Acknowledgements">Acknowledgements</a></h2>
 +
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 +
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Latest revision as of 03:42, 22 October 2009

Hi Mom

Parts

/***********************************************************************************************************************/


Biobrick part#1
Bba_K207000
PhyB N-terminal fragment sequence
Registry vector:
Plasmid: pSP3K3 ,Kan resistance
Sequence: atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct






Biobrick part#2
Bba_K207001
PhyB N-terminal fragment-DBD fusion sequence
(GAL4 Binding Domain of pGBKT7, highlighted in bold)
Registry vector:
Plasmid: pSB12A, Amp resistance
Subcloned from part # E0040
Biobrick part insert sequence:
Sequence: atggtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggag gagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagact ccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacag atcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttaca gtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgtt cacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccg ttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaat cgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggactt gactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattgga ctgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttg tggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatc tattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggtt gtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgc agttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagag tcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaa gatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttagggg atgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaa gcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaa actgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatct ATGAAGCTAC TGTCTTCTAT CGAACAAGCATGCGATATTT GCCGACTTAA AAAGCTCAAG TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG CTAGAAAGAC TGGAACAGCT ATTTCTACTG ATTTTTCCTC GAGAAGACCT TGACATGATT TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA ACATTGAGAC AGCATAGAAT AAGTGCGACA TCATCATCGG AAGAGAGTAG TAACAAAGGT CAAAGACAGT TGACTGTATC

Team

Projects

Parts

References

Lab Notebook

Ethics

Safety

Acknowledgements

Retrieved from "http://2009.igem.org/Team:Harvard/Parts"