Team:Kyoto/Calendar/24 July 2009
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- | + | __NOTOC__ | |
+ | {{:Team:Kyoto/CSS}} | ||
+ | {{:Team:Kyoto/Template:Leftmenu}} | ||
+ | {{:Team:Kyoto/CSS/notebook}} | ||
+ | <html><div id="maincontents"></html> | ||
+ | <!-- EDIT below [ここより下を編集してください] --> | ||
+ | {{:Team:Kyoto/Calendar}} | ||
+ | |||
+ | ==To Do on 24 July== | ||
+ | *Miniprep (3-O-2, 3-A-4) | ||
+ | *Restriction Enzyme Digestion | ||
+ | *Electrophoresis | ||
+ | |||
+ | ==Results== | ||
+ | ===Miniprep=== | ||
+ | *3-O-2, 3-A-4 was minipreped. | ||
+ | |||
+ | ===Digestion=== | ||
+ | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | ||
+ | |||
+ | {| border="1" style="text-align:center;width:50%" | ||
+ | |+Sample list | ||
+ | !NO.!!Sample Name!!Enzyme | ||
+ | |-align="center" | ||
+ | |1||3-O-2||Eco R1, PST1 | ||
+ | |-align="center" | ||
+ | |2||3-A-4||Eco R1, PST1 | ||
+ | |-align="center" | ||
+ | |3||3-O-2||Eco R1 | ||
+ | |-align="center" | ||
+ | |4||3-A-4||Eco R1 | ||
+ | |} | ||
+ | |||
+ | ===Electrophoresis=== | ||
+ | [[Image:Kyoto_e_090724_1.png|250px|right]] | ||
+ | * Very clear bands were found. (See the gel image in right!) | ||
+ | * The bands are listed below. | ||
+ | |||
+ | {| border="1" style="text-align:center;width:50%" | ||
+ | |+Parts and Plasmid length | ||
+ | !!!3-O-2!!3-A-4 | ||
+ | |- | ||
+ | |Parts length / bp||2837||487 | ||
+ | |- | ||
+ | |Plasmid length / bp||3266||3266 | ||
+ | |} | ||
+ | <!-- EDIT above [ここより上を編集してください] --> | ||
+ | <html> | ||
+ | </div><!--end of maincontents--> | ||
+ | </html> |
Latest revision as of 06:53, 14 August 2009
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To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |