Template:Team:KULeuven/6 August 2009/VanillinProduction

From 2009.igem.org

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Today the purification of the gel electrophorese was done on the cells from yesterday, with the nanodrop the concentration was measured:<br>
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Today the purification of the gel electrophoresis was done on the cells from yesterday.
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A1, '''Sam5''': 11,6 ng/ul <br>
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Nanodrop concentrations:<br>
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A2, '''Sam8''': 5,5 ng/ul<br>
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A1, '''''sam5''''': 11,6 ng/ul <br>
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B2, '''ComT''':  24,3 ng/ul<br>
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A2, '''''sam8''''': 5,5 ng/ul<br>
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C1, '''Fsc''':  16,6 ng/ul<br>
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B2, '''''COMT''''':  24,3 ng/ul<br>
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C2, '''Ech''':  16,9 ng/ul<br>
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C1, '''''fcs''''':  16,6 ng/ul<br>
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C2, '''''ech''''':  16,9 ng/ul<br>
A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again.
A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again.
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B1 (RBS) had failed because it was too short, however ligating B1 and B2 (ComT) was no longer needed since the part with RBS was available in the iGEM 2009 kit, {{kulpart|BBa_I742109}}.
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B1 (RBS) had failed because it was too short, however ligating B1 and B2 (COMT) was no longer needed since the part with RBS was available in the iGEM 2009 kit {{kulpart|BBa_I742109}}.
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A1 and A2 were redigested:<br>
A1 and A2 were redigested:<br>
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A1: '''Sam8''', EcoRI en SpeI<br>  
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A1: '''''sam8''''', EcoRI en SpeI<br>  
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A2: '''Sam5''', EcoRI en XbaI<br>
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A2: '''''sam5''''', EcoRI en XbaI<br>
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followed by gel electrophorese
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followed by gel electrophoresis

Latest revision as of 09:51, 14 September 2009

Today the purification of the gel electrophoresis was done on the cells from yesterday. Nanodrop concentrations:
A1, sam5: 11,6 ng/ul
A2, sam8: 5,5 ng/ul
B2, COMT: 24,3 ng/ul
C1, fcs: 16,6 ng/ul
C2, ech: 16,9 ng/ul

A1 and A2 showed a very low concentration and the 260/280 value was quite high so both were electrophoresed again. B1 (RBS) had failed because it was too short, however ligating B1 and B2 (COMT) was no longer needed since the part with RBS was available in the iGEM 2009 kit .

A1 and A2 were redigested:
A1: sam8, EcoRI en SpeI
A2: sam5, EcoRI en XbaI
followed by gel electrophoresis

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